Views
3 years ago

Kromasil CelluCoat

Kromasil CelluCoat

42 Scale up –

42 Scale up – With/without additive General facts p Frequently used in order to improve peak shape and hence increase the efficiency of the column p Analytical-prep differences in α p Complicates solvent recycling process p Stability problems, racemization, additives are accumulated during evaporation

43 Scale up – With/without additive mAU 400 MWD1 A, Sig=223,10 Ref=360,100 (BKH\060510000008.D) Analytical injections mAU 80 MWD1 D, Sig=223,10 Ref=360,100 (01BKH29\001-0401.D) O 300 N H O 60 OH 200 40 100 20 0 0 0 2 4 6 8 10 12 14 16 18 min 0 10 20 30 40 50 min Heptane/2-propanol 90/10 0.1% DEA Flow: 1mL/min, UV @ 223 nm Kromasil-10-CelluCoat 4.6x250 mm Heptane/2-propanol 90/10 Flow: 1 mL/min, UV @ 223 nm Kromasil-5-CelluCoat 4.6x250 mm Conclusion: Additive is needed for CelluCoat – a disadvantage in prep/semi prep

Kromasil Chiral – the technical evidence
Kromasil application guide
Kromasil 300 Å – for your protein separations - dichrom
Kromasil application guide - Winlab.com.au
ProntoSIL Product Catalog - MAC-MOD Analytical Inc.
Regis Chromatography Catalogue
HPLC and UHPLC Columns
Kromasil 300 Å – for your protein separations - VDS optilab
AmyCoat CelluCoat brochure - Winlab.com.au
ACE Excel brochure
Regis Chiral Stationary Phases
Kromasil_chiral_technical_evidence - Chromatographyshop
ACE C18-AR and C18-PFP - Hplc.eu
Chiral Technologies (Daicel) Catalogue 2004
ACE Excel Product Bulletin - MAC-MOD Analytical Inc.
HPLC Columns & Bulk Resins - BGB Analytik AG
Chiral technical evidence - Winlab.com.au
Gel Permeation Chromatography
Introduction Installation General Guidelines for All Jordi ... - Hplc.eu
Regis Technologies - Bujno Chemicals
Prep brochure - Winlab.com.au
Regis' Chiral Stationary Phases - Hplc.eu
Introducing Reproducible selectivity changes to HPLC.PPT - Hplc.eu
HALO RP-Amide columns
82 Reversed Phase - YMC Europe GmbH
Regis Chiral Stationary Phases
Exploiting Selectivity in HPLC and UHPLC With Rational Stationary ...