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IVIS |Kinetic

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From upper left to<br />

lower right: PC-3M-luc2<br />

Bioware Ultra metastasizes<br />

throughout the mouse. Real<br />

time images where taken<br />

during simultaneous luciferin<br />

I.V. injection. Images were<br />

taken every 57ms at EM<br />

Gain 100. The last image<br />

was taken in conventional<br />

imaging mode without EM<br />

Gain and 1s exposure time.<br />

Real-time XF680 distribution<br />

dynamics with dye alone<br />

and XF-680 conjugated<br />

to herceptin. Distribution<br />

kinetic curves of both XF680<br />

and XF680-herceptin.<br />

<strong>IVIS</strong> | Kinetic<br />

Real-Time Fluorescent and<br />

Bioluminescent Fast Imaging<br />

The <strong>IVIS</strong> Kinetic from Caliper Life Sciences provides a real time, fast<br />

im aging system enabling acquisition of biologically relevant events<br />

within milliseconds. The <strong>IVIS</strong> Kinetic can perform both quantitative<br />

luminescence and fluorescence as a standard high signal to noise<br />

imager and as a high speed imager. The system includes a highly<br />

sensitive EMCCD camera for signal enhancement and the ability to<br />

reduce exposure times enabling fast kinetics. From the leading optical<br />

imaging platform for in vivo analysis, <strong>IVIS</strong> systems are supported by<br />

a range of practical accessories developed through experience in<br />

research laboratories worldwide. The <strong>IVIS</strong> Kinetic offers a light-tight<br />

injection port which supporting a syringe injector system enabling<br />

real time compound and/or substrate administration. A removable<br />

pull-out tray is included to facilitate pre-imaging animal procedures.


<strong>IVIS</strong> KINETIC<br />

Imaging Results - Real-time Fast Imaging<br />

The <strong>IVIS</strong> Kinetic is capable of imaging both fluorescence and bioluminescence for fast kinetic applications such as<br />

millisecond calcium transients, compound and/or substrate distribution or perfusion, immune responses, pharmcokinetics<br />

in anesthetized or conscious animal models. The <strong>IVIS</strong> Kinetic allows you to overlay real time events in both<br />

photo graphic and bioluminescence or Fluorescence images simultaneously with a single camera.<br />

Figure 1. Real time luciferin distribution<br />

through P3CM-luc tumor (PC-3M-luc2<br />

Bioware Ultra). Images were taken every<br />

57ms at Bin 2/EM Gain 100 for 3 minutes.<br />

Pre-Injection 1.6s 1m 8.2s 2m 36.9s<br />

Imaging Results - Monitoring Conscious Animals is Simple<br />

Following conscious animals is simple. Evaluate single or multiple regions of interests (ROI) through time. By using<br />

an <strong>IVIS</strong> Isolation Chamber, your subjects can freely move around and perform tasks while monitoring changes in<br />

bioluminscence or fluorescence.<br />

Figure 2. Real-time images were<br />

taken of a mouse walking around<br />

an isolation chamber within the <strong>IVIS</strong><br />

Kinetic with PC-3M-luc2 Bioware<br />

Ultra Cells implanted on the thigh.<br />

Tracking ROIs define the illuminated<br />

tumor automatically throughout the<br />

image sequence.<br />

Imaging Results - EMCCD Camera Technology<br />

Take advantage of 2 instruments in one. The <strong>IVIS</strong> Kinetic has all the features you would expect from an <strong>IVIS</strong> Lumina<br />

and the EM Gain feature which significantly enhances your signal and reduce exposure times required to detect<br />

real-time luminescent or fluorescent signals. EMCCD cameras are the optimal choice in performing a wide range<br />

of fast, real time data acquisitions due its fast frame transfer chip design and overall longterm camera stability in<br />

comparison to other fast imaging technologies. EMCCD’s allows the user to perform long exposure (no EM gain)<br />

imaging procedures and fast kinetic apploications with the same camera while achieving higher quantum effeciences<br />

and low detector noise.<br />

EMoff EM50 EM100 EM250<br />

Figure 3. GAPDH-Luc Transgenic mouse 10 minutes after<br />

luciferin injection. Images are at identical settings with<br />

the exception of EM gain settings. EM gain is set to OFF<br />

(Standard Mode), 50x, 100x and 250x respectively.


<strong>IVIS</strong> KINETIC<br />

Imaging Results - Living Image 3.1 Software- Quantitative Results<br />

Living Image 3.1 for kinetic acquisitions offers the flexibility to adjust picture and overlay scaling of luminescence<br />

and fluorescence settings during acquisition. Follow real-time data development directly in living image during<br />

data acquisition.<br />

Figure 4. Images left to right: Representation of Living Software 3.1 user interface for kinetic acquisitions. Post-acquisition, ROI’s are defined<br />

at metastatic points of PC-3M-luc2 Bioware Ultra. Data is calculated and exported to almost any available statistical or graphing software.<br />

Inside the <strong>IVIS</strong> Kinetic<br />

CCD Camera<br />

• The <strong>IVIS</strong> Kinetic EMCCD is 13.3 x 13.3 mm square, with<br />

1024 x 1024 pixels 13 micron in width, yielding high<br />

resolution and amplified sensitivity.<br />

• Back-thinned, back-illuminated grade 1 CCD provides<br />

high quantum efficiency over the entire visible to<br />

near-infrared spectrum<br />

• Frame transfer technology yields rapid image readout speeds<br />

• 14 and 16 bit digitizer delivers broad dynamic range<br />

• The CCD is thermoelectrically (Peltier) cooled to<br />

-80 ºC ensuring low dark current and low noise<br />

Exchangeable<br />

Imaging Chamber<br />

Filter Wheel<br />

• Light-tight imaging chamber<br />

• High light collection lens, f /0.95 – f/16<br />

• Field of View from 0.5-12.5 cm 2<br />

• 8 position emission filter wheels<br />

• 10 position excitation filter wheels<br />

Anesthesia<br />

• 4 filter wheel choices for a broad range of<br />

Manifold<br />

fluorescence applications<br />

• LED lamps for photographic images<br />

• Heated stage to maintain optimum body temperature<br />

• Motor controlled stage, filter wheel, lens position, and f-stop<br />

• Integrated pull-out stage<br />

Optional Accessories<br />

EMCCD Camera<br />

• Gas anesthesia ports and 3 or 5 position manifold within<br />

imaging chamber allow anesthesia to be maintained<br />

during imaging sessions<br />

• Syringe injection system allows the user to acquire real time<br />

functional responses to compounds, by defining amount, flow<br />

rate and start time of the pump through Living Image 3.1.<br />

• Optional integrated ECG monitoring system<br />

• Optional 24cm FOV lens attachment<br />

Electronics Tray<br />

• Optional chiller to achieve -90 ºC<br />

High Light<br />

Collection Lens<br />

ECG<br />

Port<br />

Injector<br />

Port<br />

Light-tight<br />

Enclosure<br />

Catheter<br />

Pull-out<br />

Stage


FLUOROPHORES<br />

GFP, YFP and PKH26<br />

Cy 5.5, DsRed, dTomato<br />

and XenoFluor 680<br />

Indocyanine Green and<br />

XenoFluor 750<br />

Multiple Fluorophores Spanning<br />

500-900 nm Broad Imaging Solution<br />

*20 nm bandpass emission filter<br />

**35 nm bandpass excitation filters<br />

STANDARD HIGH RESOLUTION<br />

EXCITATION FILTER SET (BUILT-IN)**<br />

430, 465, 500,<br />

535, 570, 605,<br />

640, 675, 710, 745<br />

Optional <strong>IVIS</strong> Kinetic Imaging System Accessories<br />

EMISSION FILTER OPTIONS<br />

*500 Series<br />

500, 520, 540, 560, 580, 600 and 620<br />

*600 Series<br />

580, 600, 620, 640, 660, 680 and 700<br />

*700 Series<br />

720, 740, 760, 780, 800, 820, and 840<br />

Standard Emission Filter Set<br />

515-575, 575-650, 695-770, 810-875<br />

Cat No. 124633<br />

<strong>IVIS</strong> Syringe<br />

Injection System<br />

<strong>IVIS</strong> Kinetic Part Number 124632<br />

Cat No. 123997 XIC-3<br />

Animal Isolation Chamber Kit<br />

Cat No. 124229<br />

ECG Monitoring<br />

System<br />

Cat No. 118918<br />

XGI-8 Anesthesia System<br />

IMAGING SYSTEM COMPONENTS<br />

SPECIFICATIONS<br />

Camera Sensor<br />

Back-thinned, back-illuminated, cooled Grade 1 CCD, frame transfer<br />

CCD Size<br />

13 x 13 μm<br />

Imaging Pixels 1024 x 1024<br />

Quantum Efficiency >85% at 500 – 700 nm, >30% at 400 – 900 nm<br />

Pixel Size<br />

13 microns<br />

Min. Detectable Radiance<br />

100 photons/s/sr/cm2<br />

Min. Field of View (FOV)<br />

5 x 5 cm<br />

Max. Field of View (FOV)<br />

12.5 x 12.5 cm (optional 24 x 24 cm)<br />

Lens f/.95 – f/16, 50 mm<br />

Min. Image Pixel Resolution<br />

50 microns<br />

Linear Electron Multiplier Gain 1, 50, 100, 250<br />

Frame rate<br />

9 fps at bin=1, 36 fps at bin =2, 144 fps at bin=4, 576fps at bin=8<br />

Pixel Read out Rate<br />

10,5,3,1 MHz<br />

Amplifiers<br />

EM and Conventional<br />

Effective Read Noise (e-)<br />

6-47 (Gain and Read-out Speed Dependant)<br />

Dark Current (Typical)<br />

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