UltraVIEW VoX 3D Live Cell Imaging System

S P E C I F I C A T I O N S
UltraVIEW VoX 3D Live
Cell Imaging System
Cellular Imaging and Analysis
The UltraVIEW ® VoX is a fully integrated confocal system designed for 3D live cell imaging. Allowing cellular interactions
to be observed and measured in great detail, 3D analysis provides greater context to cellular changes and more accurate
results. Based on the Yokogawa ® CSU-X1 spinning disk confocal scanner, synchronized by PerkinElmer ® patented
ProSync ® technology and powered by our award winning Volocity ® software, this system provides exceptional speed,
quality and performance for high speed fluorescence imaging of living cells. Designed to meet the continually developing
needs of research, the UltraVIEW VoX offers scientists a wide range of system configurations and upgrade options, for
many different applications. The system combines the latest imaging hardware, with application focused software and
state of the art computing technologies, to give a complete solution from acquisition to analysis.
All systems provide the following options:
• A solid-state modular laser engine with the choice of up to six laser lines
• A selection of CCD and EMCCD cameras optimized for speed, sensitivity and resolution
• Support for upright and inverted microscopes from all major manufacturers
• Support for an extensive choice of microscope hardware accessories for 3D, multi-positional and multi-channel
imaging
• The option of Macintosh ® or Windows ® computing platforms to suit your preference, with a range of computer
models including 64-bit systems to meet your performance needs
• The latest release of Volocity Acquisition, Volocity Visualization and Volocity Quantitation software for an integrated
acquisition to analysis solution
• Software supplied with an Imaging License Server application to allow analysis software to be shared across a
network for added productivity
• Support for a wide range of advanced imaging techniques and analysis applications including FRAP, Ratio,
Restoration, TIRF and FRET
All of these options along with the significant benefits of easy-to-use high-performance 3D imaging, make the
UltraVIEW VoX the confocal instrument of choice for either a single laboratory or a multi-functional centralized facility.
PerkinElmer offers a complete system solution for your cellular imaging application that includes an expert imaging
specialist team, dedicated technical support desk and global service organization.

SPECIFICATIONS
UltraVIEW VoX System
Confocal Scanning Unit
Model
Type
Scanning speed
Synchronization
Laser coupling
Excitation range
Emission range
Camera mounts
Camera switching
Yokogawa ® CSU-X1
Microlens-enhanced Nipkow disk
Standard: 5000 rpm
High (optional): 10000 rpm
From ProSync 2 controller
Direct injection
400-650 nm
420-750 nm
Single or dual (optional)
Automated (optional)
System Synchronization
Source
ProSync 2 synchronization controller
Inputs USB 2.0
External laser safety interlock
Laser interlock key-switch
Outputs
6-channel synchronized laser control (channel and power)
Camera synchronization control
CSU synchronization control
Analog piezo Z axis control
3-channel electro-mechanical shutter control
3-port optical switch control
PhotoKinesis ® Unit control
Laser blanking
Active blanking to minimize photo-damage and photo-bleaching
Laser Excitation
Laser combiner Modular Laser System 2.0
Laser lines
Select up to 6 lines from a choice of 7 including:
Solid state 405 nm (50 mW)
Solid state 440 nm (40 mW)
Solid state 488 nm (50 mW)
Solid state 515 nm (25 mW)
Solid state 561 nm or 594 nm (50 mW)
Solid state 640 nm (50 mW)
(Powers stated are raw laser power)
Laser control
Full control over laser switching and power levels
from Volocity via ProSync 2 controller
Blanking and channel crosstalk

Dimensions
MLS2.0 supplied in up to 3 stackable cases
Control box; Lasers500 nm
498 mm x 567 mm x 104 mm (w, d, h)
Dichroic Filters
Dichroic 1
Dichroic 2
Dichroic 3
Dichroic switching
Compatible with 405 / 488 / 568 / 640 nm laser lines
Transmission in pass band >80%
Reflectance in emission band >90%
Dye compatibility includes DAPI, GFP2, GFP, mRFP, Cy5
Compatible with 405 / 440 / 514 / 640 nm laser lines
Transmission in pass band >80%
Reflectance in emission band >90%
Dye compatibility includes CFP, YFP, Cy5
Compatible with 405 - 440 / 515 / 561 nm laser lines
Transmission in pass band >80%
Reflectance in emission band >90%
Dye compatibility includes DAPI, CFP/YFP, mRFP
Automated, controlled from Volocity
Emission Filters
Emission filter position 1
Emission filter position 2
Emission filter position 3
Emission filter position 4
Emission filter position 5
Emission filter position 6
Emission filter position 7
Emission filter position 8
Empty position for high speed fast sequential
multicolor imaging
Green (single band emission discrimination)
525 nm center, 50 nm half-power bandwidth
Compatible with 488 nm laser line
Red (dual band emission discrimination)
445 nm center, 60 nm half-power bandwidth
615 nm center, 70 nm half-power bandwidth
Compatible with 405/561 nm laser lines
Cyan/Far red (dual band emission discrimination)
485 nm center, 60 nm half-power bandwidth
705 nm center, 90 nm half-power bandwidth
Compatible with 440/640 nm laser lines
Yellow (single band emission discrimination)
587 nm center, 125 nm half-power bandwidth
Compatible with 515 nm laser line
DIC analyzer
446 nm centre, 33 nm half-power bandwidth
524 nm centre, 42 nm half-power bandwidth
600 nm centre, 36 nm half-power bandwidth
677 nm centre, 28 nm half-power bandwidth
Compatible with 405 / 488 / 561 / 640 nm laser lines
Cyan/Yellow (triple band fast sequential)
477 nm center, 45 nm half-power bandwidth
575 nm center, 100 nm half-power bandwidth
705 nm center, 90 nm half-power bandwidth
Compatible with 405 / 440 / 515 / 640 nm laser lines
3

SPECIFICATIONS
Emission filter position 9
Emission filter switching
Emission filter
switching speed
Cyan / Yellow / Red (triple band fast sequential)
480 nm centre, 49 nm half-power bandwidth
536 nm centre, 24 nm half-power bandwidth
685 nm centre, 229 nm half-power bandwidth
Compatible with 405 / 440 / 515 / 561 nm laser lines
Automated, controlled from Volocity
162 ms between sequential positions fully loaded
55 ms between sequential positions unloaded
Emission filters supplied with 594 nm laser available on request
Camera Options
Hamamatsu ® ORCA-R2 Camera head Hermetic vacuum-sealed head
Dual cooling
Imaging device
Effective pixels
Cell size
Dual scan mode
Pixel clock
Readout noise
(r.m.s typical)
Full well capacity
(typical)
Dynamic range (typical)
Cooling temperature
Dark current
Exposure time
Air or water cooling
ER-150 progressive scan interline CCD
1344 (H) x 1024 (V)
6.45 µm (H) x 6.45 µm (V)
Normal scan / fast scan
14.00 MHz (normal scan)
28.00 MHz (fast scan)
6 electrons (normal scan)
10 electrons (fast scan)
18 000 electrons (HDR mode off)
36 000 electrons (HDR mode on)
3000:1 (at normal scan, 1 x 1 binning)
-35 ºC (air cooled)
-40 ºC (water cooled at 20 ºC)
0.0005 electrons/pixel/s (at -40 ºC)
1 ms to 6 s
Binning 2 x 2, 4 x 4, 8 x 8
Sub-array readout
Dual light mode
Analog gain
Analog offset
Yes
Low light / high light
Yes (10x maximum)
Yes
Interface IEEE1394b-2002 (FireWire 800)
Frame rate
QE
A/D converter
16.2 fps (maximum at 1 x 1 binning)
>15 fps (1 x 1 binning, fully synchronized)
>70% (peak)
12 bits
4

Hamamatsu ® C9100-50 Camera head Hermetic vacuum-sealed head
Proprietary to PerkinElmer
Cooling
Forced air
Imaging device
Effective pixels
Cell size
Pixel clock
Readout noise
(r.m.s typical)
Full well capacity (typical)
Electron multiplier gain max.
Cooling temperature
Dark current
Exposure time
Frame transfer EMCCD
1000 (H) x 1000 (V)
8 µm (H) x 8 µm (V)
35.00 MHz
10 electrons (at minimum EM gain)
25 fps (1 x 1 binning, fully synchronized)
>60% (peak)
Hamamatsu ® C9100-13 Camera head Hermetic vacuum-sealed head
Dual cooling
Imaging device
Effective pixels
Cell size
Pixel clock
Readout noise (r.m.s typical)
Full well capacity (typical)
Electron multiplier gain max. 1200x
Cooling temperature
Dark current
Exposure time
Air or water cooling
Back-thinned frame transfer EMCCD
512 (H) x 512 (V)
16 µm (H) x 16 µm (V)
Up to 11 MHz (EMCCD)
Up to 2.75 MHz (normal CCD)
25 electrons (at minimum EM gain,
11 MHz EMCCD)

SPECIFICATIONS
Hamamatsu ® C9100-13 Sub-array readout Yes
continued Interface Camera Link
A/D converter
Frame rate
Real time image processing
Ultra low light detection
QE
16 bits
31.9 fps (maximum at 1 x 1 binning)
>25 fps (1 x 1 binning, fully synchronized)
Background subtraction, shading correction
Photon imaging mode
>90% (peak)
Hamamatsu ® C9100-14 Camera head Hermetic vacuum-sealed head
Dual cooling
Imaging device
Effective pixels
Cell size
Pixel clock
Readout noise (r.m.s typical)
Full well capacity (typical)
Electron multiplier gain max.
Cooling temperature
Dark current
Exposure time
Air or water cooling
Back-thinned frame transfer EMCCD
1024 (H) x 1024 (V)
13 µm (H) x 13 µm (V)
Up to 11 MHz (EMCCD)
Up to 2.75 MHz (normal CCD)
10 electrons (at minimum EM gain,
11 MHz EMCCD)
8.5 fps (1 x 1 binning, fully synchronized)
Background subtraction, shading correction
Photon imaging mode
>90% (peak)
Note: The UltraVIEW VoX can can support single or multiple camera configurations including simultaneous imaging.
Computer Workstation Options
Windows ® PC (32-bit, 4 GB)
Lenovo ® S20 workstation
32-bit Windows ® XP Professional
Intel ® Core2 Quad W3520 – 2.66GHz x 4
4 GB RAM
1 TB disk storage (RAID 0)
ATI Radeon HD 5870 graphics for high-performance 3D visualization
6

Windows ® PC (64-bit, 4 GB)
Windows ® PC (64-bit, 8 GB)
Windows ® PC (64-bit, 16 GB)
Apple ® Mac ® Pro
Display options
Lenovo ® S20 workstation
64-bit Windows ® XP x64 Professional
Intel ® Core2 Quad W3520 – 2.66GHz x 4
4 GB RAM
1 TB disk storage (RAID 0)
ATI Radeon HD 5870 graphics for high-performance 3D visualization
Lenovo ® S20 workstation
64-bit Windows ® XP x64 Professional
Intel ® Core2 Quad W3520 – 2.66GHz x 4
8 GB RAM
1 TB disk storage (RAID 0)
ATI Radeon HD 5870 graphics for high-performance 3D visualization
Lenovo ® D20 workstation
64-bit Windows ® XP x64 Professional
Intel ® Xeon X5550 – 2.66GHz x 8
16 GB RAM
1 TB disk storage (RAID 0)
ATI Radeon HD 5870 graphics for high-performance 3D visualization
Apple ® Mac ® Pro workstation
Mac ® OS X
Intel ® Xeon Quad-Core “Nehalem” – 2.8 GHz x 4
6 GB RAM
2 TB disk storage (RAID 0)
ATI Radeon HD 5770 graphics for high-performance 3D visualization
24” or 30” PC HD flat panel LCD display
23” or 30” Apple ® cinema HD flat panel display
Note: We reserve the right to change the computer specifications without notice as part of our program of continuous instrument improvement.
Software
Volocity
Multi-platform 3D cellular imaging and analysis software. Compatible with
Windows ® XP, Windows ® XP x64, Windows Vista ® , Windows Vista ® x64
and Mac ® OS X. Award-winning user interface. Multi-processor aware for
high performance on multi-core hardware.
Import formats include:
• Apple ® PICT • OME TIFF
• BioRad ® PIC • Openlab LIFF
• DeltaVision ® DV • Openlab Raw
• GE IN Cell • PerkinElmer AIC
• ICS/IDS
• PerkinElmer TIFF
• JPEG
• TIFF
• Leica ® LEI • TIFF with PerkinElmer extensions
• Leica ® LIF • Till Vision
• MetaMorph ® STK • Windows ® BMP
• Olympus ® OIF • Zeiss ® LSM
• Olympus ® TIFF
Export formats include:
• JPEG
• Openlab Raw
• OME TIFF • QuickTime ® movie
• TIFF
• QuickTime ® VR
• ICS/IDS
• AVI movie (Windows ® only)
• Openlab LIFF • WMV movie (Windows ® only)
7

SPECIFICATIONS
Volocity Acquisition
Volocity Visualization
Volocity Quantitation
Imaging License Server
Volocity Restoration (optional)
Parallel processing and video streaming architecture
Acquisition direct to disk (not limited by RAM)
Extensive range of supported PerkinElmer and third-party
hardware including microscopes, stages, filter wheels, Z drives
Single or multiple channel experiments
2D or 3D image stacks
Single or multiple timepoint experiments (fixed or live cell timelapse)
XY multi-point acquisition (with a supported XY stage)
Allows data review during acquisition
Fully integrated with UltraVIEW VoX and ProSync 2
synchronization controller
Interactive rendering of 3D and 4D multi-channel datasets
Range of renders including high resolution, intensity, surface and
brightfield rendering
High quality ray tracer rendering for publication images
Independent control of channel appearance
Playback in 3D over time
Bookmarks to store and replicate viewing state
Interactive movie creation interface
Snapshot and QuickTime ® VR creation from 3D images
Stack re-slicing
Detect objects using a range of criteria such as intensity and size
Time-resolved 2D and 3D morphological and intensity measurements
Automatic 3D object tracking
Colocalization analysis of 2D and 3D datasets
Calculation of Pearson's correlation coefficient and Manders' coefficients
Manual or automatic thresholding (using the method of Costes et al.)
Identification of regions of colocalization and exclusion in 2D or 3D
with PDM channels
Analysis of full images or regions/objects of interest
Offline 3-image 2D and 3D FRET and ratio image analysis
Batch processing
Data analysis including summaries, normalization and trend analysis
Chart generation in 2D and 3D
Storage and recall of measurement and analysis routines
Export of data to Microsoft ® Excel ® and other software packages
Share Volocity licenses across multiple computers
Add additional software licenses as required
License per concurrent user rather than by machine
Maximize the return on software investment
Group and user-level security
Integrates with Windows ® Active Directory ®
Report on usage by user and by group
Reserve licenses for individual users or groups
Supports Mac ® OS X and Windows ® clients
Restore and improve confocality for both widefield and confocal images
Iterative maximum-entropy deconvolution algorithm
Fast 3D deconvolution algorithm
Real-time (video-rate) fast 3D deconvolution algorithm
Point-spread function generator for widefield, LSM, 2-photon
and spinning disk imaging systems
Point-spread measurement from acquired images
8

Volocity FRAP (optional)
Volocity Ratio Acquisition
(optional)
Imaging Computing Server
(optional)
Fully automated FRAP acquisition and analysis
(with a supported bleaching device)
Support for 3D bleaching and recovery
Fully integrated with other acquisition techniques, no need to quit
and restart
Supports multiple bleach-recovery cycles
Supports acquisition of additional non-recovery channels
Single exponential, double exponential and Soumpasis curve fitting models
Automatic calculation of T1/2, mobile fraction, immobile fraction
and diffusion coefficient
Automatic generation of recovery chart with raw data and fitted curve
Included free of charge with the PhotoKinesis Unit
Fully automated ratiometric acquisition and analysis
2D or 3D ratiometric acquisition
Live simultaneous visualization of A, B, Ratio and IMD channels
Live plotting of raw, ratiometric and IMD intensities
Online background correction and thresholding
Automatic online measurement and charting of regions of interest
High-performance cluster computing solution
Expand the cluster at any time
Compatible with Volocity restoration, ray tracing and quantitation tools
Note: Volocity software is regularly updated and the version supplied with a system order will be the current release version on the date of
dispatch. The software is cross-platform with licensing controlled using a hardware key. All systems are provided with a twelve-month software
maintenance agreement that entitles the user to free downloadable software upgrades during this period. Free upgrades apply only to the
products included with the purchase. For system upgrades, all software expiry dates must be aligned on each hardware key.
PhotoKinesis Accessory (optional)
General
PhotoKinesis Targeted Illumination Device
Scan mechanism
Dual-galvo
Scan spot size
3 sizes, software-selectable
Scan modes
Spot or arbitrary regions of interest
Calibration
Automatic
Imaging path
Straight, avoiding mirror loss and distortions
Imaging to bleaching switch time

SPECIFICATIONS
Z Positioning (optional)
XY stage piezo (100 µm)
XY stage piezo (200 µm)
XY stage piezo (250 µm)
XY stage piezo (500 µm)
Objective collar piezo
Microscope focus
XY Positioning (optional)
Automated XY stage
(inverted microscopes)
Automated XY stage
(upright microscopes)
Stage-top piezo Z
100 µm travel
1 nm repeatability
10 ms move time (typical)
Compatible with 26 x 76 mm slides and 35 mm Petri dishes
Stage-top piezo Z
200 µm travel
1 nm repeatability
10 ms move time (typical)
Compatible with 26 x 76 mm slides and 35 mm Petri dishes
Stage-top piezo Z
250 µm travel
2.5 nm repeatability
20 ms move time (typical)
Compatible with 26 x 76 mm slides, up to 70 mm Petri dishes
and 85 x 128 mm MTP
Stage-top piezo Z
500 µm travel
5 nm repeatability
20 ms move time (typical)
Compatible with 26 x 76 mm slides, up to 70 mm Petri dishes
and 85 x 128 mm MTP
Objective-mounted piezo Z
100 µm travel
30 nm repeatability (typical)
Volocity can control most automated microscope focus drives
Performance will depend on microscope model
114 x 75 mm travel

Secondary Dichroic Filters for Simultaneous Imaging (optional)
Secondary dichroic ( 484 nm )
Secondary dichroic ( 509 nm )
Secondary dichroic ( 580 nm )
Secondary dichroic ( 662 nm )
484 nm edge filter wavelengths < 484 nm to horizontal camera
wavelengths > 484 nm to vertical camera
Compatible with 405 nm laser line and ≥ 488 nm
509 nm edge filter wavelengths < 509 nm to horizontal camera
wavelengths > 509 nm to vertical camera
Compatible with 440 / 515 CFP/YFP dyes
580 nm edge filter wavelengths < 580 nm to vertical camera
compaitble with 405 or 440 or 488 nm lines imaged with
561 or 594 or 640 nm lines
662 nm edge filter wavelengths < 662 nm to horizontal camera
wavelengths > 662 nm to vertical camera
Compatible with 640 nm laser line ≤ 594 nm
Note: Simultaneous imaging configurations require the CSU - X1 - A3 confocal scanning unit and two identical cameras. Second camera kits include
camera, C - mount, filterwheel , filters and CSU - X light path controller.
Environmental Control (optional)
Stage-top incubation Temperature control from ambient to 50 ºC
Rapid temperature stabilization
Excellent long-term stability
Includes both stage and objective heater
Premix gas control (optional)
Requires automated XY stage
Full incubation
Fully encloses microscope stage and objectives
Temperature control from ambient +10 ºC (32 ºC typical) to 42 ºC
Stability +/- 0.2 ºC over 24 hours
CO 2
enrichment (optional)
Anti-vibration Table (optional)
Large isolation
Passive 750 mm x 1200 mm ScienceDesk
workstation
Custom 750 mm x 1200 mm x 60 mm breadboard
Adjustable wrist rest
Single shelf
Overhead shelf (750 mm)
Schrader valve air connection
Recommended for inverted microscope systems
Medium isolation Passive 750 mm x 900 mm ScienceDesk
workstation
Custom 750 mm x 900 mm x 60 mm breadboard
Adjustable wrist rest
Single shelf
CPU/movable shelf
Schrader valve air connection
Recommended for upright microscope systems
11

SPECIFICATIONS
Microscope Compatibility
Leica ®
Nikon ®
Olympus ®
Zeiss ®
Upright microscope
support
DMI series (inverted)
DMIR series (inverted)
DM5000/5500/6000B (upright)
TE200/300/2000 (inverted)
TiE (inverted)
Eclipse 80i/90i (upright)
IX70/80/81 (inverted)
BX51/61 (upright)
AxioVert 200M (inverted)
AxioObserver (inverted)
AxioImager A1 (upright)
Supporting stand for using an UltraVIEW VoX on an upright microscope
Allows fine XYZ positioning of the UltraVIEW VoX onto the microscope camera
port for exact alignment
Prevents damage to microscopes from heavy accessories
Tilt action for easy servicing
Note: For laser safety reasons, the UltraVIEW VoX must only be installed on a microscope that has a 100:0% switching slider between the camera
Environmental / Electrical
Supply voltage
100-240 V AC (50-60 Hz)
Operating temperature 15-40 ºC ambient
15-30 ºC with air-cooled C9100-13 or C9100-14 camera
Humidity
30-70% (non-condensing)
As part of our continuous improvement program we reserve the right to alter the specifications listed in this document.
LASER RADIATION
AVOID EXPOSURE TO BEAM
CLASS 3B LASER PRODUCT
MAXIMUM OUTPUT: 100 mW
PULSE DURATION: CONTINUOUS WAVE
WAVELENGTH RANGE: 405 – 660 nm
THIS LASER PRODUCT CONFORMS TO IEC 60825-1,
21 CFR 1040.10 & 1040.11
For further information on our technical specifications, applications and support services please
visit www.cellularimaging.com
PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2009-2010, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.
008842A_02 Sep. 2010