GeneJET™ Plasmid Miniprep Kit

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GeneJET™ Plasmid Miniprep Kit

High Quality of Plasmid DNA

The GeneJET Plasmid Miniprep Kit is designed for fast and cost-effective isolation of high quality plasmid DNA from

recombinant E.coli cultures. Up to 20µg of high copy plasmid DNA can be recovered from 1 to 5 ml of culture. Neither

phenol/chloroform extraction nor alcohol precipitation is required. The kit utilizes a silica-based membrane in the form of

a convenient spin column. The purified plasmid DNA is ready for immediate use in all molecular biology procedures.

Features

• Efficient – yields up to 20µg of high quality plasmid DNA

• Fast – procedure takes only 14 minutes

• Easy-to-use – simple procedure, color-coded bottles

• Convenient – GeneJET Spin Columns are capped and assembled with collection tubes

GeneJET Spin Columns are capped

and assembled with collection tubes

Applications

Fast and easy isolation of high quality plasmid DNA suitable

for all conventional molecular biology procedures, including:

• FastDigest DNA cleavage

• Conventional restriction digestion

• PCR, fast PCR

• Automated fluorescent sequencing

• Conventional radioactive sequencing

• Transformation

• In vitro transcription (see www.fermentas.com for recommendations)

Kit Components

• GeneJET Spin Columns assembled with

Collection Tubes

• Resuspension Solution

• Lysis Solution

• Neutralization Solution

• Wash Solution (concentrated)

• RNase A Solution

• Elution Buffer

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