Download - Facilities

Laboratory Safety 

Biology Department

Laboratory Safety 

Exposures in the Biology Laboratory: 

Dangerous Chemicals 

Biohazards / Sharps 

Electrical Hazards 

Radioactive Isotopes 

Fire 

Steam 

Animal Bites

Responsibilities of Personnel 

• Read the Biology Department Chemical 

Hygiene Plan 

• Know where the MSDS are located 

• Make sure your work practices do not 

present a danger to yourself or others 

• Complete initial training and participate in 

safety update and annual training 

• If you don’t know, ask

Signs 

Signs are posted for your information. 

Read them so you become aware of hazards in specific areas.

Space Hazard Sign 

Emergency Contact Information 

Chemical Hazard Rating 

Specific Hazards

Chemical Labels 

Chemicals that are NOT in their primary containers 

• Full chemical/reagent name 

• Abbreviation Sheet acceptable 

• Concentration 

• Percent, Molarity, Molality, 

Normality, 10X 

• Hazard class 

• Color coding acceptable 

• Target organ information 

• See MSDS posting acceptable

Material Safety Data Sheets 

Material Safety Data Sheets 

(MSDS) are required for all 

chemicals used or stored. 

MSDS for all chemicals within 

Winston Hall are located in 

Room 208 

The MSDS must be accessible. 

MSDS can not be kept in a 

locked room with limited access.

Material Safety Data Sheet - MSDS 

Responsibilities of P.I. or Faculty 

• Inform user of the chemical application, health hazards and physical properties prior to using a chemical 

• Provide MSDS to the user 

• Provide proper PPE and train users on proper use and function of PPE 

• Ensure only approved chemicals are used in the laboratory 

Responsibilities of Students working in the Lab 

• Read and understand the MSDS for chemicals used in the lab 

• Understand chemical application, health hazards and physical properties prior to 

using a chemical 

• Use required PPE 

• Do not use unauthorized chemicals within the laboratory


• Item I: Chemical Identity - The chemical identity as listed on the label, the material's chemical and 

common names, and a list of all hazardous ingredients. 

• Item II: Physical Data - Physical and chemical characteristics, such as vapor pressure, flash 

point, density, boiling point, etc. 

• Item III: Physical Hazards - Fire and explosion data. Reactivity data. These usually appear as two 

separate sections on the actual MSDS. 

• Item IV: Health Hazards - Signs and symptoms of exposure, and any medical conditions generally 

recognized as being aggravated by exposure to the material. 

• Item V: Primary Route(s) of Entry - The route(s) by which the material could most likely enter the 

body. 

• Item VI: Exposure Limits - Legal exposure limits (OSHA and other recommended limits). This 

frequently includes toxicity information.


• Item VII: Whether the Material is Carcinogenic - States whether the material has been found to be 

carcinogenic. 

• Item VIII: Precautions for Safe Handling and Use - Any precautions for safe handling and use, 

such as appropriate hygienic practices, protective measures required during handling of 

contaminated equipment, and procedures for clean-up of spills and leaks. 

• Item IX: Control Measures - Any control measures, such as engineering controls, work practices, 

or personal protective equipment. 

• Item X: Emergency and First-Aid Measures - Self explanatory. 

• Item XI: Revision Data - Date of preparation of the MSDS, and the date of the last change to it. 

• Item XII: Manufacturer Contact Information - Name, address, and telephone number of the party 

responsible for the MSDS, who can provide additional information about the material if 

necessary.

Engineering Controls 

Engineering controls are used to remove a hazard or 

place a barrier between lab personnel and the hazard. 

Emergency Showers / Eyewash Stations 

Fume Hoods 

Biosafety Cabinets 

Autoclaves 

Fire Extinguishers 

Exit / Egress


Safety shower and Eye wash must be 

accessible and unobstructed

Fume Hoods 

Fume hoods are a necessary 

element of any research laboratory.

Fume Hoods 

Face Velocity 90fpm < face velocity < 120fpm = passing 

Face Velocity 50fpm < face velocity < 79fpm or 

121fpm < face velocity < 150fpm 

Call Customer Service x4255 for repair. 

Face velocity < 50fpm or face velocity >150fpm 

DO NOT USE. Call Customer Service x4255 for urgent repair.

Fume Hoods 

Visually inspect fume hood 

Look for damage or noticeable alterations (damaged or missing sash, airfoil, etc.). 

Raise and lower sash to verify correct function 

Make sure it’s not stuck or off track 

Work Clean 

Remove excess clutter and equipment. If possible, equipment should be elevated 1 inch above 

the working surface to allow adequate airflow. 

Close the sash 

This reduces electricity consumption and reduces potential for fumes in the laboratory.


Fume hoods: 

• Contain a minimal amount of material 

• Airflow slots in the back must be clear 

• Flow reading should be minimum of 90 fpm


Blocking airflow slots, being used as a storage device, etc.. 

bad

Biosafety 

Read the 

Wake Forest University Biohazard Waste Management Plan 

prior to working with biohazardous material. 

The Plan is available on the Biology Department website. 

The Plan provides information on the following: 

General Work Practices 

Blood and Bodily Fluids 

Sharps 

On-Site Biohazard Waste Treatment 

Biohazard Waste Packaging and Storage 

Emergency Actions

Blood Borne Pathogens (BBP’s) 

• Blood Borne Pathogen (BBP) is a virus or disease that enters into the 

bloodstream, such as Human Immunodeficiency Virus (HIV) or 

Hepatitis B and C (Hep B, Hep C). 

• BBPs are transmitted by contact with body fluids: Blood, Vomit, Urine 

& Feces and through Needle Stick Injuries. 

• If you come into contact with body fluids, you must report the incident 

of exposure to your supervisor and the EHS Office @ 7189 

immediately.

Blood Borne Pathogens Exposure Control Plan 

• Hepatitis B Vaccine is offered by WFU to those who have 

the potential to become infected with a BBP. You can 

accept or deny the vaccination. 

• If you have the potential for an exposure while conducting 

your work, you must be enrolled in the Exposure Control 

Plan and trained accordingly. 

• Have your P.I. contact the EHS Office to enroll you in the 

program.

Biohazard Exposures 

The following exposures must be reported immediately to the P.I. or Lab 

manager, Christie Otten and the Environmental, Health and Safety Office. 

Needle stick 

Human Blood / Bodily Fluid 

Infectious substances 

Animal bite

Sharps 

• Great care should be used 

when using sharps to minimize 

the chance of accidental skin 

puncture. 

• DO NOT bend, recap, or 

remove from syringe the 

contaminated needle or other 

contaminated sharps. 

• Immediately after use, place 

contaminated sharps in the 

appropriate Sharps container.

Biological Safety Cabinets 

Purpose: 

Protection of lab personnel from potentially 

infectious/harmful biological materials. Also, 

protection of biological reagents from non-sterile 

environment. 

Classification Biosafety Level Application 

Class I 1 low risk biological agents 

Class II 1,2,3 low to moderate risk biological agents

Bio Safety Level 1 (BSL-1) 

• Appropriate for work with Risk Group 1 (RG-1) 

microorganisms (E. coli K-12, Bacillus subtilis) 

• RG-1 agents not associated with disease in healthy adult 

humans 

• Minimal potential hazard to lab personnel or the 

environment 

• Default BSL for chemical-only labs with no biologicals or 

toxins

Bio Safety Level 2 (BSL-2) 

• Appropriate for work with Risk Group 2 (RG-2) pathogens 

(Salmonella sp.,Listeria sp.,influenza) and potentially 

infectious blood, tissues, and body fluids 

• RG-2 agents associated with human disease of varying 

severity (rarely serious); vaccine or therapy often 

available; avoid creating aerosols 

For a complete listing of Risk Group organisms, see the following: 

http://oba.od.nih.gov/oba/rac/guidelines_02/APPENDIX_B.htm

Laminar Flow Hood 

Laminar Flow 

• Personnel Protection (NO!) 

– out-ward air flow (75 FPM) 

• Environmental Protection(NO!) 

– 100% exhaust to room 

• Product Protection (YES!)

Bio-Safety Cabinet 

BSC: Class I 

• Personnel Protection(Yes!) 

– in-ward air flow (75 FPM) 

• Environmental Protection(Yes!) 

– HEPA filter and/or activated 

charcoal filter 

– 100% exhaust to room 

• Product Protection(NO!) 

BSC: Class II A 

• Personnel Protection(Yes!) 

―in-ward air flow 

• Environmental Protection(Yes!) 

―HEPA filter and/or activated 

charcoal filter 

• Product Protection(Yes!) 

―Down-ward flow of HEPAfiltered 

air

BSC: Class II A

Bio Safety Cabinets 

Always be sure your Bio Safety 

Cabinet has been certified before 

you use it! 

Certification is required on an annual basis. 

Certification date must be indicated on the cabinet.

Autoclaves 

• Chemical indicator strips indicate that 

item has been exposed to sterilizing 

conditions, not that it is sterile 

• Commonly use steam heated to 121 °C 

for 15 minutes or 134 °C for 3 minutes. 

• Steam WILL cause burns. Use caution 

when opening. 

• Wear autoclave gloves to protect your 

hands from steam and heat.

Autoclaves 

Prior to using the autoclave in Winston Hall, 

make sure you have been trained on its use 

by your P.I. 

The autoclave is temperamental. If it is not 

treated correctly it will set off the fire alarm in 

the building.


Electrical panel must be accessible and doors must open fully (90 o ) 

Machine is blocking 

the Electrical Panel 

(Also, a desk has been 

placed beneath the 

Emergency Shower.)

Electrical Safety 

If a breaker trips within a laboratory, please call 

Customer Service at X4255 to have it re-set. 

Do not try to re-set breakers. There is the potential for 

an arc flash (electrical explosion) whenever a 

breaker is re-set. 

Second and third degree burns 

from MINOR arc-flash incident.


Gel Electrophoresis 

It is commonly believed that there is little hazard in electrophoresis 

apparatus use except when operating at high voltages required for 

procedures such as DNA sequencing. 

However, even agarose gel electrophoresis 

operating at 100 volts can cause a lethal shock at 

a current of 25 milliamps.


How to Protect Yourself while Performing 

Gel Electrophoresis 

• Turn the power off before connecting the electrical leads. 

• Connect one lead at a time using one hand only. 

• Insure that your hands are dry while connecting the leads. 

• Keep the apparatus away from sinks or other water sources. 

• Turn off power before opening lid or reaching inside chamber. 

• Don't override safety devices. 

• Don't run electrophoresis equipment unattended. 

• NEVER, EVER, NEVER HOT LOAD.


Fire Doors must remain closed or be on automatic closures. 

You know it’s a Fire Door if it’s 

spring loaded (self-closing). 

Do not prop open Fire Doors. 

That’s a violation of the Fire 

Code. 

That would be bad.

Fire Extinguisher 

Know where the nearest fire extinguisher 

is located before you start working. 

BE AWARE: 

– General purpose for ordinary 

combustibles, flammable liquids and electrical fires. 

– for use on fires in rooms with 

sensitive electronic equipment (i.e. Electron 

Microscopy, Confocal).

Fire Extinguisher 

1. Pull the Pin 

(Metal or Plastic Ring at Top of Fire Extinguisher) 

2. Aim at the Base of the Fire 

(Stand 8-10 feet from fire) 

3. Squeeze Handle and 4. Sweep Back 

and Forth

Egress / Aisle Space 

Exit Door obstructed 

Less than 44 inch egress 

Combustibles in egress (couch)

Compressed Gas 

Gas Cylinders: 

• Properly stored and secured 

• Do not store incompatibles together 

(i.e. Hydrogen and Oxygen) 

Gas Cylinders MUST be 

chained at all times and capped 

when not in use. 

Unchained 

cylinders 

Unchained, uncapped, 

Hydrogen cylinder


Chemical storage shelves must 

have adequate seismic protection 

Vacuum system flasks must be 

labeled and protected (taped, 

plastic, parafilm, mesh, etc.)

PPE 

Personal Protective 

Equipment (PPE) 

It’s worn to protect 

YOU.

Personal Protective Equipment 


• Identify hazards that may require protection, both chemical and physical 

• Complete an accurate description of the tasks requiring PPE and review with student 

• Provide proper PPE and train users on proper use and function of PPE 

Responsibilities of student 

• Understand the hazards that may require protection, both chemical and physical 

• Complete training with P.I. or faculty member prior to working in the Lab 

• Know the correct PPE to use for the situation 

• Use the proper PPE at all times

Choosing the Proper PPE 

Here is what the P.I. or Faculty member needs to review about PPE with the 

student prior to working in the lab: 

Nature of potential contact 

Splash potential 

Occasional or continuous immersion 

Spray / mist / vapors / dust 

Contact location 

What part of the body will be affected 

Eyes, face, nose, mouth, body, feet 

Contact time 

Length of time will influence selection of PPE

Choosing the Proper PPE 

Here’s more stuff the P.I. or Faculty member needs to review about PPE with 

the student prior to working in the lab: 

Hazards of skin contact 

Read the MSDS 

Limitations of the PPE 

When will it not work. What is breakthrough time 

What could go wrong 

What do I do if my glove rips 

What to do if PPE fails 

Should I panic 

When to dispose of or decontaminate PPE 

How long can I safely wear nitrile gloves before they become ineffective

Types of PPE 

Hand Protection (gloves) 

Eye Protection (safety glasses, goggles) 

Body Protection (lab coat) 

Foot Protection (closed-toed shoes)

Hand Protection 

COMMON GLOVE TYPES: 

• Latex 

• Nitrile 

• Butyl, PVA, Vinyl 

• Autoclave 

• Cryogenic 

• Silver shield

When using Gloves 

• Choose proper gloves 

• Wash hands regularly 

• Inspect gloves before and during use 

• All gloves are porous -CHANGE REGULARLY 

• Do not re-use disposable gloves 

DO NOT WEAR GLOVES 

OUTSIDE THE LAB

Eye Protection 

• Safety glasses 

• Goggles 

• Face shield

Body Protection 

• Laboratory coat (typical lab) 

• Aprons (grossing station, milling)

Foot Protection 

Foot Protection 

Closed-Toed Shoes

YOU ARE NOT ALLOWED TO WEAR 

THESE IN THE LAB – EVER. 

OPEN TOE / OPEN TOP 

SHOES PROHIBITED IN THE 

LABORATORY

Unapproved Shoes 

I love them!!! 

But not a good 

choice for work 

in a laboratory!

Hazardous Material Storage 

The following are chemicals that must be properly stored: 

• Flammables 

– 4 liters or less allowed on benchtops in lab. 

– >4 to

Flammable Storage 

Flash Point - the lowest temperature at which a liquid will give off sufficient 

vapor to ignite on contact with an ignition source. 

You may store common alcohols in the refrigerator. 

These include: 

Chemical 

Flash Point 

Ethanol 55.4 °F 

Methanol 54 °F 

Isopropanol 51.8 °F


The following are chemicals that must be properly stored: 

Acids 

Bases 

Oxidizers 

Toxics


Common Incompatible Materials 

Acids and Bases 

Flammable and Oxidizers 

Flammables and Poisons 

Water Reactives with anything else

Chemical Incompatibility Matrix 

Acids, 

Inorganic 

Acids, 

Oxidizing 

Acids, 

Organic 

Alkalis 

(Bases) 

Oxidizers 

Poisons, 

inorganic 

Poisons, 

organic 

Water 

reactives 

Organic 

solvents 

Acids, 

Inorganic 

Acids, 

Oxidizing 

Acids, 

Organic 

Alkalis 

(Bases) 

X X X X X X 

X X X X X X 

X X X X X X X 

X X X X X X 

Oxidizers X X X X 

Poisons, 

inorganic 

Poisons, 

organic 

Water 

reactives 

Organic 

solvents 

X X X X X X 

X X X X X X 

X X X X X X 

X X X X X 

X = incompatible


The following are chemicals that must be properly stored: (Cont.) 

• All controlled substances 

Example: Testosterone, Sulfonmethane, N-Benzylpiperazine 

See http://www.deadiversion.usdoj.gov/schedules/orangebook/c_cs_alpha.pdf 

• Select Agent Toxins 

Example: Bacillus anthracis, Botulinum neurotoxins, Ricin 

See http://www.selectagents.gov/Select%20Agents%20and%20Toxins%20List.html 

• Potentially unstable materials 

Example: Ethyl ether, Tetrahydrofuran, Picric acid


CONTROLLED SUBSTANCES 

Example: Testosterone, Sulfonmethane, N-Benzylpiperazine 

See http://www.deadiversion.usdoj.gov/schedules/orangebook/c_cs_alpha.pdf 


• Ensure accurate log is kept of Controlled Substances under P.I. license 

• Ensure a secure, locked location under the control of the P.I. is available for Controlled Substances 

• Provide proper training to students on proper use and storage of Controlled Substances 

Responsibilities of student 

• Complete training with P.I. prior to working with Controlled Substances 

• Know the hazards associated with the Controlled Substance 

• Report any mishandling or misuse of Controlled Substance to P.I. or Department 

Chair.

Preparedness and Prevention 

• Glass chemical bottles stored properly and not on the floor 

• All flames must be attended (Bunsen burner) 

• General housekeeping is a requirement 

• All food and beverages must be kept in clean areas (not in the lab) 

• Refrigerators / freezers in Winston Hall designated for food must be 

marked “FOOD ONLY”

Preparedness and Prevention 

• Chemical Spills 

– Notify others, and evacuate and secure the area 

– Notify the P.I. or Faculty director of the laboratory 

– Call Environmental, Health and Safety Office at x3089 and Campus 

Police at x5911 

– Important Information to have: 

• Chemical name 

• Exact location 

• Amount spilled 

• Phone number 

• Know the location of the chemical spill kit and how 

to use it

Small Spill Clean Up 

Small chemical spills may be cleaned up by lab 

personnel. 

Certain protocols must be followed. 

DO NOT attempt to clean up spill if: 

•There is a chance of inhalation hazard from fumes, 

dust or aerosol 

•There is potential for fire 

•It is carcinogenic or highly toxic 

In these instances evacuate the area and call Christie 

Otten and Environmental, Health and Safety Office at 

x 3089.

Small Chemical Spill Clean Up 

Protocol for small chemical spill clean up in the laboratory 

• Evacuate area of spill and cordon off area to keep others out 

• Notify P.I. or Faculty director of laboratory and Christie Otten 

• Obtain spill response kit from the main stairwell 

• Obtain MSDS from Room 208 

• Don correct PPE, including gloves, lab coat, booties and eye 

protection as indicated on the MSDS 

• Pour absorbent over the spill to cover 

• Scoop absorbent into empty pail 

• Carefully scoop any broken glass into the pail 

• Close the pail and label contents 

• Remove PPE – Gloves last! 

• Call Environmental, Health and Safety Office to pick up pail.

Chemical Exposure 

• If someone in the lab has an exposure to the eyes or skin, direct 

them to the closest eyewash or safety shower and flush the area for 

15 minutes. 

• If an inhalation exposure occurs, remove the victim to fresh air and 

call x5911 for medical assistance. 

• Put on any necessary PPE to protect yourself before providing aid. 

• Unless absolutely necessary, do not leave the victim alone. Stay 

with them until medical help arrives. 

• Obtain a copy of the MSDS for the chemical and send with the victim 

when they are transported for medical evaluation or treatment. 

All chemical exposures must be reported immediately to the P.I. or Faculty 

director, Christie Otten and the Environmental, Health and Safety Office.

Hazardous Waste Collection 

Each laboratory has a designated area for the 

collection of hazardous waste. 

• Size of the container(s) may vary, but each Hazardous Waste container 

must have: 

– Label with the words “HAZARDOUS WASTE” 

– Contents of the container - write out the chemical name. 

– NO ABBREVIATIONS.

Hazardous Waste Label 

Hazardous Waste 

Alcohol Collection Container 

Materials that may go into this container include: 

Methanol Isopropanol Acetone 

Ethanol Propanol Butanol 

Isobutanol Hexanol Heptanol 

Octanol Nonanol Decanol 

Amyl Alcohol 

**** DO NOT MIX **** 

OXIDIZERS OR ACIDS IN THIS CONTAINER 

Hazardous Waste 

Alcohol Collection Container 

Materials that may go into this container include: 

Methanol Isopropanol Acetone 

Ethanol Propanol Butanol 

Isobutanol Hexanol Heptanol 

Octanol Nonanol Decanol 

Amyl Alcohol 

**** DO NOT MIX **** 

OXIDIZERS OR ACIDS IN THIS CONTAINER

Good Job! 

Marked 

HAZARDOUS WASTE 

Contents clearly marked

Bad Job. 

Nice try. 

Not marked as 

HAZARDOUS WASTE

Waste Collection 

When collecting waste keep in mind: 

Only compatible waste may go in the same container: 

DO NOT MIX 

Acids and Bases 

Flammables and Oxidizers 

Cyanides and Acids 

Water-reactive with anything

What not to do… 

• Open Container 

• No Label 

• Potential overflow to 

sewer (sink)

Waste Management 

• All waste and chemical containers must be kept securely 

capped when not in use 

• That means if you are not putting waste in the container at that 

exact moment, it must be closed. 

Leave about one inch of 

headspace at the top of the 

container to allow for 

expansion.

Pick-up Request 

When your container is ready to be removed, complete the Hazardous 

and EtBr Waste Pick-up Request Form and send to Christie Otten. 

Request 

date 

Chemical Name or Item 

Hazardous and EtBr Waste Pick-up Request 

Location: 

PI and Rm # 

Solid 

or 

Liquid 

Type of 

Container Approx 

* . Amt Qty

Time Bomb 

What not to do…

Gross neglect / Improper Chemical Storage 

What not to do…

Biohazard Waste 

• Infectious (biological) waste must be properly managed 

• Needles, razor blades, and other sharps must be disposed of in 

an approved Sharps container 

Hi! My name is 

APPROVED 

SHARPS 

CONTAINER 

When container is near full, contact Shannon Mallison for a 

pick-up and replacement.

What not to do… 

Overfilled Sharps Container 

Serological pipettes in 

regular trash

Waste Management 

• Non-contaminated broken glass, pipettes, and 

pipette tips must be disposed of in a lined box

Do not overfill glass containers 

What not to do…

Radioactive 

Prior to working with radiological isotopes, users must 

read and understand the Radiation Safety Manual. 

The Manual must be kept in the laboratory where the 

isotopes are used at all times. 

• Spills of radioactive isotopes require special methods for clean up. 

• If a spill occurs, notify the P.I. or Faculty director and Christie Otten 

immediately. 

• If possible, do not leave the area until decontamination is 

completed. This will limit the chance of tracking radioactive material 

throughout the building.

Radioactive Waste Disposal 

Radioactive Waste disposal is handled through the WFUBMC 

Radiation Safety Office. 

For all questions regarding disposal of radioactive waste contact: 

David Howell – Radiation Safety Officer 

336-716-1202

Specialized Areas 

• Dark Room, Warm Room, Cold Room 

– Chemical Hazards, Oxygen Deficiency 

• Histology / Morphology 

– Blood 

• Machine Operations (centrifuges) 

– Mechanical Hazards, Electrical Hazards 

• Electron Microscopy Labs 

– Osmium tetroxide 

– Cyanide stains 

• Animal Holding Area 

– Bites 

– Viral, Fungal, Parasitic Infections

Emergency Procedures 

• Emergency telephone list by the phone 

• Lab personnel must know: 

– Where to evacuate in case of fire 

– How to use a fire extinguisher (PASS) 

– Proper procedures in case of injury, 

chemical spill, or biological spill 

– How to contact Campus Police and EHS 

Office

• 21CFR 1301.75 Registration of Manufacturers, Distributors, and Dispensers of 

• Controlled Substances 

• 21CFR 1301.75 Current Good Manufacturing Practice for Finished Pharmaceuticals 

• 29 CFR 1910.1450 Lab Standard 

• 29 CFR 1910.1200 Hazard Communication 

• 29 CFR 1910.1030 Bloodborne Pathogens 

• 29 CFR 1910.334 Electrical: Use of Equipment 

• 29 CFR 1910.151 Medical Services and First Aid 

• 29 CFR 1910.134 Respiratory Protection 

• 29 CFR 1910.133 Eye and Face Protection 

• 29 CFR 1910.157 Portable Fire Extinguishers 

• 29 CFR 1910.303 Electrical: General 

• 29 CFR 1910.36 Design and Construction Requirements for Exit Routes 

• 29 CFR 1910.253 Oxygen: Fuel For Welding 

• 29 CFR 1926.350 Gas: Cutting and Welding 

• 29 CFR 1910.57 Maintenance, Safeguards, and Operational Features for Exit Routes 

• 29 CFR 1910.138 Hand Protection 

• 29 CFR 1910.136 Occupational Foot Protection 

• 29 CFR 1910.106 Flammable and Combustible Liquids 

• 40 CFR 262.34 Accumulation Time 

• 40 CFR 262.11 Hazardous Waste Determination 

• 40 CFR 262.33 Subpart D: List of Hazardous Wastes 

• 40 CFR 262.177 Specific Requirements for Incompatible Waste 

• 40 CFR 262.173 Management of Containers 

• 40 CFR 262.171 Condition of Containers 

• 40 CFR 262.370 Community Right to Know: Inventory Reporting 

• 42 CFR 72 and 73 Possession, Use, and Transfer of Select Agents and Toxins 

• NFPA 1 Uniform Fire Code 

• NFPA 45 Fire Protection for Laboratories Using Chemicals 

• NFPA 55 Storage, Use, and Handling of Compressed Gases and Cryogenic Fluids 

• NFPA 30 Flammable and Combustible Liquids Code 

• NFPA 101 Life Safety Code 

• NFPA 10 Portable Fire Extinguishers 

• NFPA 25 Inspection, Testing, and Maintenance of Water-Based Fire Protection Systems 

• National Institutes of Health - Guidelines for Research Involving Recombinant DNA Molecules 

Regulatory Key

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