Product Catalog - ALA Scientific Instruments

Product Catalog 

2012/2013 

Furthering Life Science through Innovative Instrumentation

ALA Scientific Instruments, Inc. 

ALA Scientific Instruments, Inc. began as Adams and List Associates Ltd. in 1986. It was founded by 

Jonathan J. Adams, a holocaust survivor, and Jurgen List, a German businessman and president of List 

Medical and List Electronic GmbH. In the beginning, Adams and List Associates’ primary purpose was to 

import and market the products of List Medical and List Electronic in the U.S. In 1988, Alan Kriegstein 

joined Adams and List as the General Manager and a year later he obtained the title of President. In 

1990, Andrew Pomerantz joined the company as Vice President. Kriegstein and Pomerantz quickly realized 

that for the company to grow and prosper it must develop its own product line. ALA’s first product 

was a system to internally perfuse patch pipettes. This was quickly followed by the DAD-12 drug application 

device and the TLCMI temperature control system. 

The company moved to Westbury, NY in June of 1991 and a small manufacturing facility was set up. Additional 

products from other manufacturers were added to the product line. In 1995, Kriegstein and 

Pomerantz took full control of the company and changed the name to ALA Scientific Instruments, Inc. 

By 2009, ALA Scientific outgrew its Westbury, NY facility and moved to a new 10,000 sq. ft building in 

Farmingdale, NY where the company is presently located. ALA designs and manufactures many systems 

and devices that are used in thousands of labs all over the world. In addition to ALA’s own products, the 

company represents several world renowned manufacturers featuring some of the finest and most sophisticated 

electronic instruments used for electrophysiology and biophysics. 

ALA Scientific’s goal is to help further scientific research with innovative instrumentation. 

Mailing Address 

60 Marine Street 

Farmingdale, NY 11735 

Phone 

631-393-6401 

Fax 

637-393-6407 

Email 

Sales@alascience.com 

Website 

www.alascience.com

Index 

Solution Delivery...................................................................... 3 

Solution Delivery Accessories.................................................9 

Perfusion Accessories.............................................................11 

Perfusion Tools........................................................................12 

Imaging and Recording Chambers....................................... 13 

Temperature Control............................................................... 14 

Fluid Control........................................................................... 17 

Specialized Electrophysiology.............................................. 18 

Pipette and Electrode Holders.............................................. 20 

Pipette Processing and Mounting Systems........................ 21 

MEA Accessories....................................................................22 

Imaging................................................................................... 23 

Rig Essentials.........................................................................24 

1

Solution Delivery 

VC 3 Systems: 4 and 8 Channel Valve Controlled Perfusion Systems 

ALA Scientific’s 4 and 8 channel perfusion systems fulfill the basic requirements of 

the student lab but can also be optimized to fit the most demanding applications of patch 

clamp recording and/or imaging. All VC 3 systems provide electronic control of solution 

exchange which improves reproducibility over manual methods. These systems are designed 

to minimize electronic noise so sensitive electrophysiological recordings can be 

performed. 

Key features of the VC 3 4 and 8 channel systems include: 

•Choice of low-maintenance pinch valves or fast-response solenoid valves 

•Customizable reservoir sizes and materials (see page 10 for options) 

•Bath or focal perfusion applicability 

•DAQ control via analog or TTL input 

•Valve control through DAQ, VC 3 software, or controller 

•Spill sensor with automatic shutoff to protect microscope optics 

•MilliManifold and MicroManifold® outlet options for low volume and rapid applications (see page 11) 

•Valve open and close timestamps via analog or digital output 

VC 3 systems have the option of integrating either pinch or Lee solenoid valves. Three-way pinch valves have response times 

of 15-20 ms and are easy to clean because solution runs through the valve in removable tubing. Lee solenoid valves have 

response times of 1-2 ms and are made for high speed applications. (images on page 9) 

VC 3 systems can drive fluid by using gravity or air pressure. 

Gravity driven systems are ideal for use with larger diameter tubing and can be used to 

perfuse bath preparations to maintain viability and establish experimental conditions. 

Gravity systems include Minimanifold, valve manifold with Lee solenoid or pinch 

valves, 60 ml reservoirs, VC 3 controller, power supply, luer valves, magnetic stand, spill 

sensor, manual flow control valve (FCV-1), DB9-BNC cable, USB cable, and software. 

Pressure driven systems can perfuse solution through 

tubing with very small ID’s, therefore, are ideal for use 

in focal applications where only a small amount of solution 

is needed around the preparation. This focal application 

is especially useful for researchers looking 

for dose response curves. Pressurized systems include 

valve manifold with Lee solenoid or pinch 

valves, 5ml pressurized reservoirs, VC 3 controller, 

power supply, magnetic stand, spill sensor, DB9-BNC 

cable, USB cable, and software. Quartz MicroManifold® 

(QMM) perfusion outlet and compression fittings 

(CF), used to minimize solutions to one outlet, as well 

as PR-10 Pressure Regulator, are sold separately. 

2 

Complete 4-channel gravity driven system 

Complete 4-channel pressure driven 

system with optional compression fittings 

and QMM


Unlike other competitive perfusion systems, all VC 3 systems include 

Windows compatible command software which allows users to set protocols 

for opening and closing of specific channels for preset amounts of 

time. Protocols can be initiated by starting within software or by external 

trigger from data acquisition systems. Individual valves of the VC 3 systems 

can also be controlled via analog or TTL input. 

Valve Controller 

Specifications 

Dimensions/Weight 

Fuse 

Computer Control Input 

Computer Output Monitoring 

Manual Control 

Valve Manifolds 

Pinch Valves 

Pinch Valve Response Time 

Pinch Valve ID Tubing 

Lee Solenoid Valves 

Lee Solenoid Valve Response Time 

8”/20.32cm x 6”/15.24cm x 2.5”/6.35cm; 1.5lbs/0.68kg 

15V DC/ 3.15 A 

Analog input 0.5V/valve; TTL high 1bit/valve; USB 2.0 command 

Analog output 0.5V/valve; Sync TTL high; TTL high 1 bit/valve 

Membrane switch 1/valve; momentary on/off or toggle on/off 

12V DC/0.25A each - 3-way normally open/normally closed pinch valve 

15-20 ms 

0.040”/1mm 

12V DC/0.08A each - Lee OEM Inert Solenoid Valve 

1-2 ms 

Gravity flow rate specifications assuming 60ml 

reservoirs are full and FCV-1 valve is not 

attached 

Height 

(cm) 

w/ Millimanifold 

(ml/min) 

90 11 37 

80 10 31 

70 9 29 

60 8 25 

50 7 22 

w/ Minimanifold 

(ml/min) 

Sample pressurized flow rate: 

1ml in 9 minutes 

at 10 PSI/ 70kPa 

through QMM with 100µm ID tubes 

VC3-4PG 

VC3-4SG 

VC3-8PG 

VC3-8SG 

VC3-4PP 

VC3-4SP 

VC3-8PP 

VC3-8SP 

Ordering Information 

4 pinch valves, 4 x 60ml reservoirs, VC 3 controller, power supply, 4 luer valves, magnetic stand, reservoir bracket, 

spill sensor, MMF-4, FCV-1, DB9-BNC4 cable, and command software 

4 Lee solenoid valves, 4 x 60ml reservoirs, VC 3 controller, power supply, 4 luer valves, magnetic stand, reservoir 

bracket, spill sensor, MMF-4, FCV-1, DB9-BNC4 cable, and command software 

8 pinch valves, 8 x 60ml reservoirs, VC 3 controller, power supply, 8 luer valves, magnetic stand, reservoir bracket, 

spill sensor, MMF-8, FCV-1, DB9-BNC8 cable, and command software 


bracket, spill sensor, MMF-8, FCV-1, DB9-BNC8 cable, and command software 

4 pinch valves, 4 x 5ml reservoirs, VC 3 controller, power supply, 4 luer valves, magnetic stand, reservoir bracket, spill 

sensor, DB9-BNC4 cable, and command software 


bracket, spill sensor, DB9-BNC4 cable, and command software 

8 pinch valves, 8 x 5ml reservoirs, VC 3 controller, power supply, 8 luer valves, magnetic stand, reservoir bracket, spill 

sensor, DB9-BNC4 cable, and command software 


bracket, spill sensor, DB9-BNC4 cable, and command software 

See page 9 -10 for additional accessories and parts for VC 3 systems. 

3


Octaflow II Multi-function Multi-valve Perfusion System 

ALA Scientific’s Octaflow II is our 

most advanced drug application device 

for electrophysiology and imaging 

research. This fast perfusion 

system is designed to meet the 

needs of basic science and drug discovery 

researchers. Software control 

of all perfusion parameters makes 

the OctaFlow II TM superior to all others. 

Octaflow II systems allow for 

choice of 8, 16, 24, or 32 reservoirs 

which can be configured in different 

modes depending on experiment. 

Constant perfusion can be achieved 

through the combination of compound 

application and wash solution 

for minimization of solution convergence 

in manifold. Periodic application of compounds can be achieved through specific focal application. U-tube and J-tube configurations 

are possible. 

Like the VC 3 systems, the Octaflow II systems can use either pinch or Lee solenoid valves.Three-way pinch valves have response 

times of 15-20 ms and are easy to clean as solution runs through the valve in removable tubing. Lee solenoid valves have 

response times of 1-2 ms and are made for high speed applications (images on page 9). The Octaflow II TM can have up to four 8- 

channel valve manifolds. Perfusion output minimization of the Octaflow II TM can be done in a variety of configurations depending 

on experimental setup. Perfusion manifolds include additional flush valve ports for optimal preparation viability and establishment 

of experimental conditions. 

Key features of the Octaflow II include: 

•Choice of low-maintenance pinch valves or fast-response solenoid valves 

•Valve-control of solution flow instead of motorized manipulation of barreled pipettes or flowing bath plates,minimizing 

sample loss and bath cross-contamination following solution exchange 

•Keyboard solution selection, for exchange “on the fly” 

•Trigger in/out for synchronization with data acquisition systems 

•Preprogrammed solution delivery through software 

•Rapid flush mode for easy cleaning 

•Programmable pressurization of fluids for optimal solution flow 

•Programmable analog outputs for valve identification, step number, or pressure information to data acquisition 


OctaFlowXXY/Z: 

XX = # of valves: specify 8, 16, 24, or 32 valves; 

Y = type of valve: order S for custom Lee solenoid valve or P for 3-way pinch valve; 

Z = # of QMM MicroManifolds® included - Order 1, 2, 3 or 4 where: 

1 = QMM type is same as the number of valves ordered - 1 QMM included with 1 flush valve 

2 = QMM-16 is included as base and 1 x QMM-8 for 24 valves or 1 x QMM-16 for 32 valves (each with additional flush valve port) 

3 = QMM-8 is included as base and 1 x QMM-8’s for 24 valves or 1x QMM-8 and 1 x QMM-16 for 32 valves (each with additional 

flush valve port) 

4 = 4 x QMM-8’s (each with additional flush valve port) 

4 

Examples of ordering code: 

OctaFlow32P/4: 32 channel pinch valve system with 4x QMM-8 and 4 flush valve ports 

OctaFlow24S/2: 24 channel solenoid valve system with 1x QMM-16, 1 x QMM-8 (each QMM with additional flush valve port)


The Octaflow II is a fully 

software-controlled system. 

The software provides five 

areas of functionality detailed 

below. 

1) Valve and reservoir configurations 

can be saved for 

future experiments. Customizations 

include unique 

pressures and labels for individual 

reservoirs as well 

as unique voltages for individual 

valves. 

2) Perfusion sequence 

setup includes parameters 

such as valve open times, 

duration, flush time, delay 

time, and output trigger in a 

flexible, easy-to-use spreadsheet 

format. 

3) Program run control allows for initiation and termination of protocols while monitoring progress and configuration 

settings. 

4) Manual control can be achieved through individual activation. Hot keys are integrated for on-the-fly control without 

protocol setup. Manual sequences can be saved for use as future macros. 

5) Additional tools, available for greater scope of performance, initiate automated cleaning sequences, select global 

software settings, and create log files. 


Max. Number of Perfusion Valves 32 

Max. Number of Valves per Bank 

8 valves/bank, up to 4 banks 

Max. Current Output per Valve 

100mA (Lee Solenoid), 350 mA (Pinch) 

Max. Voltage per Valve 

18 Volts 

Max. On-time per Valve 

no limit 

Min. On-time per Valve 

2ms @12V; 1ms @ 18V 

Max. Input Pressure 

3970mmHg - other units selectable 

Max. Pressure Applied 

~800mmHg 

Max. Number of Sequence Steps 300 

Max. Number of Sequence Event with Looping 300 20 

Number of Step Functions in Repeat Mode 

∞ 

Max. Suction Developed via Internal Venturi Pump 199 mmHg nominal 

Typical Speed of Pressure Rise 

520 mmHg/sec 

Software Compatibility Windows XP/2000/ME/7 (32- and 64-bit); USB 2.0 

Power Compatibility 120/220V 

OctaFlow Interface 

19” x 3.5” x 10” rack mountable, 10lbs/4.5kgs 

Programmable Voltage Range 

1-18 Volts/Valve 

Sample Flow Rate 

1ml in 9 min. @ 520 mmHg with standard QMM 

Standard QMM 

8 tubes @ 100µm ID, 9th tube @ 200µm ID, 100µm ID tip 

5


µFlow Micro-volume Perfusion System 

ALA Scientific’s µFlow addresses the growing need to 

preserve precious compounds during electrophysiology 

and imaging experiments. ALA has accomplished this 

through adaptation of our unique Micromanifold® technology 

for the perfusion of very low solution volumes to 

cells and tissues. 

Key features of the µFlow-4/8 include: 

•Extremely low dead volume from reservoir to tip (


ALA Scientific’s 2PK+ Patch Pipette Perfusion Kit is the original 

kit for perfusing pipettes internally during patch clamp recording. An indispensable 

tool for pharmacological and second-messenger studies of ion 

channel function, the 2PK+ is a proven system with a track record of publications 

in peer-reviewed literature. The principle behind the 2PK+ allows the 

2-port pipette holder to balance between pressure and suction so that small 

amounts of solution can be perfused through quartz capillary tubing to the tip 

of a glass pipette while suction is applied to the back. Forces are balanced to 

minimize turbulence and to maintain the established gigaseal. 

Key features of the 2PK+ include: 

•Pressure and vacuum controller to balance pressure on patch while 

driving fluid flow 

•Quartz capillary tube that provides optimal noise reduction and 

durability for proximal positioning to pipette tip 

An optional rotating carousel, MRC-6, for multiple easy solution exchanges is also available. Up 

to six sample reservoirs can be held on the MRC-6 which can easily be exchanged by rotating the 

bottom of the carousel. Each reservoir includes a Pt wire to minimize junction potentials. 

ALA Scientific’s MS-Stage and Chamber (see page 12) and Märzhäuser Wetzlar’s MM-33 shown above. 

MRC-6 


Dimensions 8” x 7.5” x 3.5” 

Wt:2 lbs 6 oz. 

Power (regulated) 9V DC / 200mA 

Input Pressure 15psi/1bar min. 

50psi/3.45bar max. 

Output Range +/- 40 mmHg 

Accuracy 

+/- 0.2 mmHg 


2PK PLUS Pipette perfusion kit with pressure/vaccum controller, perfusion 

pipette holder, and tubing/accessories kit (specify amplifier) 

2PK 30GA 30 Gauge replacement needles - package of 100 

2PK RES Set of 3 extra 0.125ml reservoirs 

2PK +PC Perfusion pressure cell (includes 3 reservoirs) 

2PK +T Butane torch 

2PK P/V 2PK+ Pressure/Vacuum Generator with output tubing 

MRC-6 Multi-Reservoir Carousel (includes 3 reservoirs) 

QT-100 Quartz Glass - 20 pieces 100µm ID x 4” (other ID’s available) 

PPH-2P-AXU Straight Axon Universal Perfusion Holder w/ Ag wire, 2 ports 

PPH-2P-BNC Straight Perfusion Holder w/ Ag wire, 2 ports - BNC type 

PPH-OTHERS Other/Custom Pipette Holders Available 

ALA Scientific’s Simple Perfusion Kits (SPK) are low-cost 4 and 8 channel perfusion 

kits. Typical applications include intra- and extracellular recording, patch clamping, 

and imaging applications. The SPK is especially useful for all types of bath perfusion applications 

and for student labs where gravity driven perfusion is ideal. 

Key features of the SPK include: 

•Ease of assembly 

•Manual Flow Control pinch valve which allows user to equilize flow output from any 

reservoir to the desired flow rate 

•Replaceable parts 

See page 9-10 for additional accessories and replacement parts 


SPK-4 4- channel kit includes magnetic stand, reservoir bracket, 4 x drip chambers, tubing, 

MMF-4, FCV-1, 4 x 3-way luer valves, and additional acccesories 

SPK-8 8- channel kit includes magnetic stand, reservoir bracket, 8 x drip chambers, tubing, 

MMF-8, FCV-1, 8 x 3-way luer valves, and additional acccesories 

SPK-DRIP Replacement drip chambers - pack of 10 

FCV-1 

7


ALA Scientific’s High Speed Solution Exchange System 

(HSSE-2/3) is an easy to use valve-based solution exchange device that 

exchanges solutions in microseconds. This is comparable to expensive piezoelectric 

devices, but at a much lower cost. 

Key features of the HSSE-2/3 include: 

•Optional micro front end for use in tight microscope 

spaces (shown below) 

•Highly reproducible perfusion time 

•20-80% rise-times of less than 100µs with open pipettes 

•Easy integration with most data acquisition and experimental 

control systems 

•ALA’s widely used VC 3 controller 

The HSSE-2/3 provides the precise, high-speed solution exchange necessary 

for mechanistic studies of ligand-gated ion channels without the cost associated 

with piezo-based systems. 

Either of three tubes can supply solution to the preparation as seen in the diagrams 

above. Solution A or Solution B can flow either to the preparation or to waste. A single 

valve operates in two positions allowing A to flow to the preparation and B to 

waste or vice versa. An additional valve controls the flow of the waste solution 

which flows to the preparation when neither solutions A or B are flowing to the 

preparation. Solutions A and B can originate from any 4 of 8 total reservoirs. 

Standard Front-end 

Optional Micro Front-end 

ALA HSSE-2/3 

ALA HSSE-UFE 


High Speed Solution Exchange System 

Optional micro-front end for HSSE 

8 

Valve Controller Dimension 

Power 

Valve Control 

Manual Control 

Pinch Valves 

Front-End Tubing 

4-Way Pinch Valve Tubing 

Reservoirs 


8”/20.32 cm x 6”/25.24cm x 2.5”/6.35cm; 1.5lbs/0.68kg 

15VDC/3.15A 

Analog input 0.5V/valve;TTL high 1bit/valve; USB 2.0 command 

Membrane switch 1/valve; mom on/off or toggle on/off 

3 x 12VDC/0.25A ea.- 3-Way Pinch Valve 

1 x 12VDC/0.25A ea.- 4-Way Pinch Valve 

Teflon, Standard: ID=300µm, OD=760µm; Micro: ID=100µm OD=230µm 

3/32 OD x 1/32 ID 

8 x 60ml syringe - Luer lock type

Solution Delivery Accessories 

ALA Scientific’s Pre-heater/ Debubbler 

can be used in two different 

ways when added to any ALA Valve 

Manifold (VM). It can function as a 

pre-heater for gravity-driven systems. 

It can also be used for de-bubbling 

solutions in focal perfusion systems 

where bubbles cause recording difficulties. 


VM-4GHEAT/DEGAS For 4-channel gravity valve manifolds 

VM-4PHEAT/DEGAS For 4-channel pressure valve manifolds 

VM-8GHEAT/DEGAS For 8-channel gravity valve manifolds 

VM-8PHEAT/DEGAS For 8-channel pressure valve manifolds 

Heater Resistance 

Power Requirements 

Temperature Range 

Temperature Accuracy 


10Ω 

24V/5A 

21 o -40 o C in ~15min 

+/-1 o C 

ALA Scientific’s 4 or 8 Channel 

Bubbler System infuses perfusion 

solutions with CO2/O2 mixtures to 

regulate carbonated buffers and ensure 

proper oxygen content. ALA 

makes it easy to infuse critical gases 

with bubbler manifolds that drop into 

perfusion reservoirs with rod mounted 

slide brackets. Each bubbler comes 

with high quality air stones, 2-way luer 

valves, tubing, and a slide bracket. By 

using the oxygen-safe PR-10 regulator system in conjunction, 

you are assured the right pressure every time. 


BUBBLER-4 4-channel kit includes 4 airstones, bracket for VMstand, 

4 x 2-way luer valves, and air manifold 

BUBBLER-8 8-channel kit includes 8 airstones, bracket for VMstand, 

8 x 2-way luer valves, and air manifold 

ALA Scientific’s PR-10 Pressure 

Regulator is an oxygensafe 

dual regulator system that 

completely removes pressure 

fluctuations at the output. It is 

used to control pressure for precise, 

fast, focal drug delivery or 

for use in controlling air pressure for the bubbler system. It 

controls output pressures from 0-10 psi. Bleed and pressure 

modes are used to completely de-pressurize the pressure 

reservoirs to prevent in-gassing when not in use. The PR-10 

includes a dual scale pressure guage and luer fittings for the 

inlet and outlets. It can be placed right next to the microscope 

or anywhere else for easy reach, thus allowing ergonomic 

integration. 

PR-10 


0-10 PSI pressure controller 

ALA Scientific’s 3-Way Pinch 

Valves have one normally 

open and one normally closed 

port which users can choose to 

use depending on their experimental 

conditions. Long-term 

perfusion experiments can utilize 

the normally open port while 

faster perfusion times can take advantage of the normally 

closed port. These easy-to-clean valves allow users to 

change solution tubing which is ideal when sticky ligandbased 

solutions are being used and daily cleaning is not sufficient. 

Pinch valves have response times of 15-20 ms. 

PV 

ALA Scientific’s Lee custommade 

Solenoid Valves are 

inert valves which can be incorporated 

into high-speed perfusion setups 

with response times of 1-2ms. 

Solution comes directly in contact 

with the internal valve which allows 

for faster perfusion times than 

pinch valves. 


L2V 2-way Lee Solenoid Valve (used in VC3 and Octaflow II 

systems) 

L3V 3-way Lee Solenoid Valve (used in Octaflow II systems - 

flush valve) 

L3VB 3-way Lee Solenoid Valve with base and LED 

ALA Scientific’s Valve Manifolds 

(VM) are available in a variety 

of configurations which can 

be added or swapped within existing 

set-ups of the VC3 or 

Octaflow II* systems. Both pressurized 

and gravity driven valve 

manifolds with either pinch of Lee solenoid valves are available. 

All manifolds include DB-9 cable, magnetic stand with 

rods, and necessary tubing. Additionally, gravity-driven valve 

manifolds include a reservoir bracket and 60ml reservoirs, 

while pressure driven valve manifolds include 5ml pressure 

reservoirs and the air manifold. 

VM-4PG 

VM-4PP 

VM-4SG 

VM-4SP 

VM-8PG 

VM-8PP 

VM-8SG 

VM-8SP 


3-way normally open, normally closed pinch valve 


all include magnetic stand, reservoir bracket, and tubing 

4-chan. pinch valve gravity manifold w/ 60 ml reservoirs 








* only pressurized manifolds compatible with Octaflow II 

9

Solution Delivery Accessories 

Small Parts and Accessories Replacements 

VC3-4C (4-channel) or VC3-8C (8-channel) 

VC3- PS 

VC3-4TUBE (4-channel) or VC3-8TUBE (8-channel) 

4- or 8- channel valve controller including power supply and DB9 cable 

VC 3 controller power supply 

4- or 8-channel tubing kit - PVC/silicone tubing w/ connectors (no MMF) 

VC34AIRMAN (4-channel) or VC38AIRMAN (8-channel) 4- or 8-channel pressurized air manifold tubing set 

VC3-BREAK 

FCV-1 

VM-STAND 

Breakout board to VC 3 controller for up to eight 12V valves 

Flow control manual adjusted pinch valve 

Magnetic base stand for valve manifolds - 2 rods and magnetic base 

SPILSNSR Replacement spill sensor with connector for use with VC 3 

DB9F-BNC4 (4-channel) or DB9f-BNC8 (8-channel) TTL trigger to 4 or 8 BNC cable for use with VC 3 

DB9-CABLE 

SFK-11 

3WLV 

Compression Fittings 

Tubing 

VC3 controller to valve manifold replacement cable 

Miscellaneous tubing and luer fittings kit 

3-way luer valve (all female ports) 

CF-1 

Compression fitting for PE-10/FEP-1 tubing to 1/16” ID tubing 

CF-2 

Compression fitting for PE-20/FEP-2 tubing to 1/16” ID tubing 

CF-3 

Luer lock compression fitting for PE-50/60 

CF-4 


CF-5 


CF-6 

Luer lock compression fitting for PE-10 

TUBING-1 

1/8” ID x 1/4” OD PVC tubing - 10’ (for PR and CPM pressure input) 

TUBING-2 

1/16” ID x 1/8” OD PVC tubing - 10’ (for PR, CPM pressure output, MMF) 

TUBING-3 

VC 3 silicone pinch valve tubing - 2’ (gravity systems) 

TUBING-4 

VC 3 silicone pinch valve tubing - 2’ (pressurized systems) 

TUBING-5 Replacement silicone tubing for HSPC headstage - 10’ 

TUBING-6 

HPT-2A tubing - 10 x 12” pieces 

TUBING-7 

PVC tubing - 4mm OD x 2mm ID x 1mm wall - 3m (PDES output) 

TUBING-8 

PVC tubing - 6mm OD x 4mm ID x 1mm wall - 3m (PDES input) 

FEP-1 Clear Teflon tubing - 230μm ID/ 600 μm OD - 4m (pressurized VC 3 ) 

FEP-2 Clear Teflon tubing - 380μm ID/ 760 μm OD - 4m (pressurized VC 3 ) 

PET-10 

Polyethylene tubing PE-10 - 4m (for 2pk+ or perfusion pipette holders) 

PET-20 

Polyethylene tubing PE-20 - 4m (for 2pk+ or perfusion pipette holders) 

10 

Reservoirs 

1MLRESCLP 

5MLRESCLP 

10MLRESCLP 

60MLRESCLP 

60MLSYRING 

5MLRESGLASS 

10MLRESGLASS 

60MLRESGLASS 

1MLRES 

5MLRES 

10MLRES 

60MLRES 

PPLG5ML 

PPLG10ML 

PPLG60ML 

1ml reservoir mounting clips - set of 4 with screws 




Box of 60ml syringes - 25 count 

5ml glass reservoir with 3-way luer valve and pressure top 



1ml pressurized reservoir assembly with 3-way luer valve 




5ml pressurized reservoir plug only - for HSW Norm-Ject luer lock tip 

10ml pressurized reservoir plug only - for HSW Norm-Ject luer lock tip 

60ml pressurized reservoir plug only - for HSW Norm-Ject luer lock tip

Perfusion Accessory 

ALA Scientific’s perfusion manifolds are used for solution exchange applications that range from slow whole bath solution exchange 

to focal application of compounds. They can be used with both gravity- or pressure-driven perfusion systems. 

ALA Scientific’s Quartz MicroManifold® (QMM) technology incorporates 

quartz micro-capillaries coated with polyimide to make them flexible and damage 

resistant. They are designed for use with pressure driven systems. Quartz 

capillaries are used to converge several perfusion inputs into a single output with a 

dead volume of ~ 90nl. Whether using to focally apply solution or puff droplets of solution, 

the QMM allows for reproducible applications while still being low-maintenance. 

QMM’s are the lowest dead volume perfusion manifolds available anywhere! 

QMM’s are available with FEP tubing attached to each inlet channel which, when 

used in conjunction with a compression fitting (see page 10), allows each channel to 

be attached to a specific valve on the VC 3 pressurized system, thus forming a complete 

system. QMM’s can also be incorporated into existing perfusion set-ups for solution 

minimization. QMM’s are quite versatile and are offered in both 100 or 200µm 

ID’s. Users also have the option of ordering QMM’s with an additional flush tube 

(200µm ID) for rapid “dead space” clearing. Replaceable tips also extend the useful 

life of the QMM. 

ALA Scientific’s MilliManifold (MLF) employs similar technology to 

the QMM and is ideal for focal applications when applied solution volume isn’t critical 

to the experiment. Larger diameter tubing (ID: 500 µm) allows for larger delivery 

volumes and lower probability of clogging. Despite an increase in diameter 

from the QMM, dead volumes can be maintained as low as ~5µl. 

ALA Scientific’s MiniManifold (MMF) provides the same quality product for bath 

solution applications with larger diameters (ID: 1.75mm) specifically used for gravity driven 

perfusion systems. 

Key features of ALA’s perfusion manifolds include: 

•Compatibility with VC 3 pressurized perfusion systems 

•Customizable number of inlet ports 

•Autoclavable materials which are inert, robust, and flexible 

•Easy inlet connections on MLF and MMF via 1/16” barbed fitting 

•Manipulator holders for easy mounting onto a manipulator 


QMM MLF MMF 

Material Polyimide coated Polyimide tubing Polyimide tubing 

quartz 

ID/ OD 

100μm/ 230μm or 500μm/900μm 1.75mm/1.85mm 

200μm/ 350μm 

Flush ID/OD 200μm/ 350μm n/a n/a 

Overall Length 3.25”/82.5mm 4”/10cm 4”/10cm 

Tip Length 5mm 0.59”/15mm 0.63”/16mm 

Dead Volume ~90nl MLF-4: ~5μl 

MLF-8: ~6μl 

MMF-4: ~310μl 

MMF-8: ~550μl 

Tubing 

Connections 

Silicone sleeves 1/16”/1.57mm 

barb fittings 

1/16”/1.57mm 

barb fittings 

Holder Ordering Information 

HOLDER-QMM QMM holder with 3-6mm diameter 

mounting bar 

HOLDER-MLF MLF holder with 3-6mm diameter 

mounting bar 

HOLDER-MMF MMF holder with 3-6mm diameter 

mounting bar 

MLF and MMF Ordering Information 

MLF-4 4-to-1 millimanifold 

QMM 

QMM Ordering Information 

_ 

ex.: QMM-8/1WT 

MLF-8 

MLF-16 

MMF-4 

MMF-8 

8-to-1 millimanifold 

16-to-1 millimanifold 

4-to-1 minimanifold 

8-to-1 minimanifold 

Number of Channels 

4, 8, 16, 24, 32 

Flush Valve 

/1= includes additional 

flush valve 

blank= does not include 

flush valve 

Tubing 

WT= with FEP tubing 

installed 

blank= FEP tubing 

not installed 

11

Perfusion Tools 

M version 

MM version 

ALA Scientific’s MS-Stage and Tool Holders are ideal for positioning electrodes 

and perfusion devices within the small confines of a microscope stage preparation. 

Tools are available in high- or low-profile versions with mounting cubes sizes of 1” and ½”, 

respectively, for accommodation to different setups. All tools have magnetic bases. 

MUTOOL/MMUTOOL-H: Universal tool holder accommodates any item that can be 

mounted on a 2.25 mm I.D. stainless steel rod. Shown with optional TS-1 thermistor (see 

page 16) 

MPIOH/MMPIOH-S: Perfusion port holder with luer lock-fitting steel cannula. 

MPSALT-H: Agar bridge holder securely positions agar bridge (2.0 mm O.D. glass tubing) 

to movable chamber containing 8mm Ag/AgCl pellet disc. Available on M-version base 

only. 

MCLIP/MMCLIP: Alligator clip with swivel and extension arm. 

TILTPORT: Tilt-a-Port magnetic perfusion with thumbscrew z-adjustable inlet/outlet port 

with grounding lug. Available on M-version base only. 

LLSHOLD: Magnetic holder for LeveLock sensor which enables control of fluid level 

and elimination of turbulence. Available on M-version base only. 

UPIOH-S: Ultra-low (¼”) perfusion port holder with luer lock-fitting steel cannula. 

MS-MAGHD: Hold down clamps for MS chambers 

MSS-EXT: Extension plate mounts onto MS-STAGE to hold long tools 

12 

MS-STAGE: Steel magnetic mounting plate offers large positioning surface and multiple 

tapped holes to securely anchor ALA’s tool holders or other laboratory made devices. 

Central opening for use with all ALA MS chambers or 35mm culture dishes. 

Easily mounts on standard upright or inverted microscopes stages including the 

ALAMSP (see page 21), Gibraltar, Narishige ITS, and Scientifica stages.

Imaging/Recording Chambers 

ALA Scientific’s MS Imaging and Recording Chambers secure 

tissue or cell preparations on coverslips. Dual compressible o-rings secure coverslips 

without adhesives. Compatible with MS-Stage, Heating and Cooling MicroIncubation 

System (HCMIS) (see page 14), and other 35mm specimen 

holders. 

Options include: 

•Coverglass sizes of 12, 18, and 25 mm 

•Stainless steel bottom for temperature control or Delrin bottom for 

imaging only purposes 

•Chamber walls or open sides 

•Perfusion ports 


MS-5 

Chamber style 

02 = chamber that uses 24/25mm round cover 

glass with a 0.75”/19mm round internal opening 

08 = chamber that uses 18mm round cover glass 

with a 0.5”/12.7mm round internal opening 

12 = chamber that uses 12mm round cover glass 

with a 0.325”/8.26mm round internal opening 

18 = chamber that uses 24/25mm round cover 

glass with a 0.75”/19mm x 0.375”/9.5mm oval internal 

opening 

Chamber bottom style 

S = stainless steel bottom, excellent when used for temperature control 

D = black Delrin bottom, excellent when used for imaging only 

Perfusion ports 

P = perfusion ports 180° apart with 1/16” barbed fittings 

X = not used, no perfusion ports 

Chamber walls 

W = remove chamber walls for excellent electrode access 

X = not used, chamber walls not removed 

Perfusion port walls 

W = remove perfusion ports base and fittings 

X = not used, perfusion ports with walls 

ALA Scientific’s Harp Slice Grids (HSG) are glass coated steel rings with polyimide 

coated silica fibers or nylon mesh available in a variety of configurations for suitability 

on most preparations. These durable and inert materials prevent drifting of submerged slices 

without impeding superfusate access. HSG are ideal for combining multielectrode array 

recording with conventional recording and stimulation methods. 


HSG-__ Silica fibers 1mm apart with ~50-65 μm 

thickness (* indicate exception) 

HSG-MEA-___ Tan nylon mesh with ~1mm2 spacing 

*HSG-5G White polyester mesh with ~800μm2 spacing 

*HSG-5H White nylon mesh with ~1mm2 spacing 

Options include: 

•Single or double rings for increased 

weight 

•Oval or round interior for different 

perfusion setups 

•Parallel or grid string pattern 

•Resistant silica coating 


Part Description Part Description 

HSG-5A 9.5mm ID x 12mm OD x 0.5mm thick; 0.2g - 5 pack HSG-MEA-5A 9.5mm ID x 12mm OD x 0.5mm thick; 0.2g - 5 pack 

HSG-5AD Same as above but 1.0mm thick and 0.4g - 5 pack HSG-MEA-5AD Same as above but 1.0mm thick and 0.4g - 5 pack 

HSG-5B 9.5mm ID x 12.75mm OD x 0.83mm thick; 0.4g - 5 pack HSG-MEA-5B 9.5mm ID x 12.75mm OD x 0.83mm thick; 0.4g - 5 pack 

HSG-5BD Same as above but 1.64mm thick and 0.8g - 5 pack HSG-MEA5-BD Same as above but 1.64mm thick and 0.8g - 5 pack 

HSG-5C 6.4mm ID x 8mm OD x 0.5mm thick; 0.05g - 5 pack HSG-MEA-5C 6.4mm ID x 8mm OD x 0.5mm thick; 0.05g - 5 pack 

HSG-5CD Same as above but 1.0mm thick and 0.1g - 5 pack HSG-MEA5-CD Same as above but 1.0mm thick and 0.1g - 5 pack 

HSG-5D 6.0x3.3mm ID x 12.7mm OD x 0.54mm thick; 0.18g - 5 pack HSG-MEA-5D 6.0x3.3mm ID x 12.7mm OD x 0.5mm thick; 0.17g - 5 pack 

HSG-5DD Same as above but 1.07mm thick and 0.35g - 5 pack HSG-MEA-5DD Same as above but 0.8mm thick and 0.35g - 5 pack 

HSG-5E 4.8x2.5mm ID x 9.5mm OD x 0.5mm thick; 0.1g - 5 pack HSG-MEA-5E 4.8x2.5mm ID x 9.5mm OD x 0.6mm thick; 0.4g - 5 pack 

HSG-5ED Same as above but .85mm thick and 0.2g - 5 pack HSG-MEA-5ED Same as above but 1.0mm thick and 0.8g - 5 pack 

HSG-5F 10.0mm ID x 13.8mm OD x 0.8mm thick; 0.18g - 5 pack HSG-MEA-5F 10.0mm ID x 13.8mm OD x 0.8mm thick; 0.14g - 5 pack 

HSG-5FD Same as above but 1.8mm thick and 0.33g - 5 pack HSG-MEA5-FD Same as above but 1.5mm thick and 0.31g - 5 pack 

HSG-5G 9.5mm ID x 12mm OD x 0.5mm thick; 0.2g - 5 pack HSG-5H 9.5mm ID x 12mm OD x 0.5mm thick; 0.2g - 5 pack 

13

Temperature Control 

ALA Scientific provides a broad range of temperature control devices and controllers for electrophysiological and imaging 

experiments done at non-ambient temperatures. 

ALA Scientific’s Bipolar Temperature Controller (HCT-1) is 

a single-channel heating and cooling temperature controller with an additional 

external feedback channel. When using the external sensor, the HCT-1 can 

detect if the sensor has been removed from the bath or if the bath has run 

dry, thus switching temperature feedback to the internal thermistor of the device 

being heated. 

Key features of the HCT-1 include: 

•Three speeds of operation for varying set-point reach times 

•Alarm mode for detection of missing sensor or dry bath 

Specification 

Dimensions 8.5” x 7.5” x 3.75” 

Weight 

3.2lbs 

Max. Voltage Output 12V 

Voltage Input 24V 

Max. Amp Output 2A 

Amp Input 

4A 

Sensor Type 2252 Thermistor 

Sensor A Connection 9 pin DIN connector 

Sensor B Connection 2mm standard monojack 

HCT-1 

TS-M 


Single-channel heating and cooling temperature controller 

Standard thermistor temperature probe with monojack connector 

ALA Scientific’s Heating and Cooling Microincubation 

Stage (HCMIS) is a microscope-mountable temperature control device 

for electrophysiology and imaging preparations. The HCMIS not only heats 

or cools the base of the chamber or petri dish, but can alter the CO 2 /O 2 mixture 

around preparations to control pH. 


HCMIS Heating/Cooling MicroIncubator stage 

HCMIS-MAGP Magnetic top plate for HCMIS 

Key features of the HCMIS include: 

•Easy accommodation of ALA’s MS chambers or a 35mm Petri dish 

•Base compatibility with most microscope stages and platforms - no 

adapter plates needed 

•In-line temperature control of perfusate 

•Minimal mechanical noise due to low profile and built-in water 

cooling ports 

•Temperature feedback from built-in thermistor 

•Gas ports for oxygenation and/or pH control of preparation 

•Compatibi with ALA’s HCT-1 and npi electronic’s PTC controllers 

14 


Dimension & Weight 

127x116x16 mm LWH - 375g 

Max Volts/Amps 

14.4V/3.7A 

Perfusion Tubing 

PTFE - 2.4mm ID x 3.8mm OD 

Min./Max. Temperature 0ºC to 55ºC 

Perfusion tubing recommended flow rates 

0 to 2ml/min 

Recommended heat sink water flow 

>300ml/min 

Typical temperature ramp speeds using npi peltier controller, 

block control 

25ºC to 13ºC - 2:30 min. / 25ºC to 37ºC - 1:45 min. gain/limiter = 75%, 

integral = 0% 

Typical temperature ramp speed w/ npi peltier controller, block 

control, MS502SW chamber, 1ml fluid, heat sink water flow, 

bath probe monitoring 

22ºC to 10ºC - 2:30 min. / 22ºC to 37ºC - 1:45 min. gain/limiter = 75%, 

integral = 0% 

Microscopes and platforms supported w/o adapter plates Nikon TESR stage, Olympus IX stages w/110mm opening, Zeiss frame K, 

Exfo Gibraltar, Narishige ITS, Scientifica - other stages available 

Cable information 

8 conductor; 2 for Peltiers, 2 for built-in thermistor, 4 unused, with DIN 

connector


ALA Scientific’s Coolit is a rapid low-noise heat sink for ALA’s heating and 

cooling devices. 

Key features of the Coolit include: 

•Manual pump speed adjustment 

•Variable temperature alarms 

•Automatic or manual radiator fan speed adjustment 

Material 


Alumnium Chassis & radiator, Acrylic, ABS 

COOLIT 


Liquid Cooling Circulator 

Dimensions 

Weight 

7.7” x 3.35” x 16.34” (19.5cm x 8.5cm x 

41.5cm) 

4.8lbs (2.2kg) 

ALA Scientific’s Heating and Cooling Perfusion Cube 

(HCPC) and Heated Perfusion Cube (HPC-2A) are compact 

and low-volume in-line temperature control devices made of ceramic coated 

aluminum. This allows direct contact with flowing solution for optimal 

control.The HCPC has the functionality to both heat and cool solutions, unlike 

the HPC-2A, which can only heat solutions. These devices are ideal for use 

on a microscope stage, with their optional magnetic stands (ALAMHOLD- 

HCPC or ALAMMT-HPC) which can easily be mounted onto ALA’s MS-Stage. 

HCPC with 

MHOLD-HCPC 

Key features of the HCPC and HPC-2A include: 

•Small footprint 

•Flow rates up to 5ml/min 

•Adjustable internal volume from 100-200µl 

•Built-in temperature sensor in fluid path 

•Temperature changes at 1 o C/sec 

•Compatiblity with ALA’s HCT-1 and npi electronic’s temperature controllers 

HPC-2A with 

MMT-HPC 


HCPC 

HPC-2A 

Weight 90g with cable 75g with cable 

Cable Length 1.2m 1.2m 

Connector 8 pin DIN 8 pin DIN 

Thermistor 2252Ω @ 25 o C 2252Ω @ 25 o C 

Max. Power 7V, 3Amps ; Peltier element 12V 

Power Element n/a 10Ω 

Max. Output 21 Watts 14 Watts 

Volume ~200μl, adjustable to 100μl ~200μl, adjustable to 100μl 

Temperature Min.: 0 o C Max.: 75 o C Max.: 75 o C 

Max. Flow Rate ~5ml/min @1m height gravity ~5ml/min @1m height gravity 

feed, adjustable down to 

0.5ml/min 

feed, adjustable down to 

0.5ml/min 

Dimensions 16 x 20 x 67mm 14 x 19 x 40mm 


HCPC Heat/Cool Perfusion Cube 

HPC-2A Heated Perfusion Cube 

MHOLD-HCPC HCPC magnetic stand holder 

MMT-HPC HPC magnetic stand holder 

15


ALA Scientific’s Heated Perfusion Tube (HPT-2A) provides the same 

function as the HPC-2 but for higher flow rates while still minimizing electrical noise. 

HPT-2A 

MMT-HPT 

Key features of the HPT-2A include: 

•Easy to replace Teflon or stainless steel internally glass coated tubing for 

simple maintenance 

•Compatibility with ALA’s HCT-1 and npi electronic’s temperature controllers 


Weight 

170g with cable 

Cable length 1.2m 

Connector 

8 pin DIN 

Thermistor 

2252Ω @ 25oC 

Power 

~2Watts 

Power Element 4-5Ω 


Max. Output 

3V 

Heating perfusion tube with integrated thermistor, 

Volume 

~1ml 

flexible cable, and DIN connector Max. Temperature 

45oC 

HPT magnetic stand holder 

Max. Flow Rate 

~9ml/min @1m height gravity feed, adjustable 

down to 0.5ml/min 

For high flow rates 4-5 volts can be used, but system must be shut down before soluion stops flowing to 

avoid meltdown of heater core 9not covered under warranty) See manual for special precautions about 

use of HPT-2A in high-wattage, high-flow mode. 

ALA Scientific’s Objective Heater features a resistive heater and built-in 

temperature sensor in a Velcro strap for wrapping around any size objective. The 

objective heater is excellent for removing cold spots caused by oil or water immersion 

objectives when performing electrophysiology or imaging experiments under 

temperature control. Cable connection to temperature controller sold separately. 


Thermistor 

2252Ω @ 25 o C 

Max. Power 12V 

Power Element 22Ω 

Connector 

Straight 4-pin keyed 

Heater Dimensions 1” x 4” 


OBJ-HEATER Objective heater with velcro 

adjustment 

OBJ-CABLE cable connection to 8 pin 

DIN ports 


HEATINGPAD-1 Mouse Heating Pad 

OBJ-CABLE cable connection to 8 pin DIN ports 

ALA Scientific’s Heating 

Pad for mice actively maintains 

an animal’s body temperature at 

pre-set target value for in vivo 

research. This homeothermic 

blanket system includes a heating 

pad, and TS-1 thermistor 

probe. The probe is a feedback 

sensor which provides the temperature 

of animal body to the 

temperature controller. Cable 

connection to temperature controller 

sold separately. 


Thermistor 

2252Ω @ 25oC 

Max. Power 12V, 10Ω resistor 

Max. Output 12V; 14Watts 

Connector 

8 pin DIN 

Pad Dimensions 125 x 65 x 1mm 

Max. Temperature 75 o C 

Materials 

Silicone Rubber 

Weight 

75g with cable 

Cable Length 1.2m 

16 

TS-1 

TS-2 

THERDIN 

MSNPICABLE 

POW-BAN 

DIN/DINCABLE 

Temperature Control Accessories 

Standard thermistor temperature probe with 2-pin Molex connector 

Miniature thermistor temperatuer probe with 2-pin Molex connector 

Replacement thermistor with 2-pin Molex to 5-pin DIN ports 

Cable to connect thermistors and heater coil to 8 pin DIN ports 

Power cable with Molex connector and banana plugs 

DIN to DIN connector extension cable - 6’/ 2m

Fluid Control 

ALA Scientific’s LeveLock (LL-2) is the only commercial fluid level controller 

designed for the sensitive environment of the patch clamp rig. Maintaining constant 

fluid levels is key for the reduction of stray capacitance and noise as well as for 

consistent focus during imaging experiments. The optical fiber sensor transmits infrared 

light to sense changes in level while eliminating electrical noise. Aspiration 

occurs below fluid level to minimize turbulence which enables use of insulating films 

on bath surface. 

Key features of the LL-2 include: 

•Aspiration rates up to 8 ml/min 

•Fluid control within 100µm of desired level 

•Level sensor with small footprint to fit into small volume chambers 

•Optional magnetic holder (LL-SHOLD) for use on MS-Stage (see page 12) 


LL-2 Chamber fluid level controller 

LL-2FILTER 20μm waster filter replacement - set of 10 

LL-SHOLD Sensor holder with adjustment control 


Controller Size 

13.5 x 13.5 x 5.1cm 

Weight 

0.77lb/350g 

Power Requirement 15V, 1A 

Nominal length of fiber optic 5”/12.7cm 

Sensor cable 1.5m 

Flow rate 

0-8ml/min with Lee solenoid valve 

Vacuum requirement -2.5psi/-17.2kPa 

OD of fiber optic sensor 0.0195”/0.5mm 

IR frequency 

950nm (peak) 

Sensor material 

Lucite/Acrylic 

ALA Scientific’s Vacuum Waste Kit (VWK) serves as an ideal suction source 

for light-duty suction jobs involving small cell chambers. The VWK can be incorporated 

easily into any set-up and is frequently used with the LeveLock system. 

Key features of the VWK include: 

•Maximum vacuum of -2.5psi (-17.2kPa) 

•Quiet and compact aspiration pump 

•Storage capacity of up to 2L 

VWK 


Vacuum waste kit includes storage container and stand 


Dimensions 

30 x 25.4 x 23cm 

Power Requirement 110/220V AC, 3.5W 

Storage Container 

2L flask 

Weight 

3.2kg/7lb 

Tubing Diameter 

1/16” x 1/8”; 1.6mm x 3.2 Tygon 

Max. Vacuum 

-2.5psi/-17.2kPa 

17

Specialized Electrophysiology 

ALA Scientific’s High Speed Pressure Clamp (HSPC-1) 

is the only commercially available instrument that generates accurate pressure/vacuum 

waveforms for the study of mechanosensitive ion channels 

and inner ear hair cell stimulation. The HSPC-1 includes a small and lownoise 

headstage for easy integration into patch clamp set-ups. The headstage 

contains a piezoelectric valve that partitions pressure and vacuum 

from a source and contains a pressure sensor for feedback control and 

display of internal pressure or vacuum. 

Key features of the HSPC-1 include: 

•Small dual pressure/vacuum headstage for mounting near 

amplifier probe 

•Moisture sensor to prolong headstage life 

•Compatibility with all major patch clamp hardware/software 

•Improved consistency of establishing gigaseals in whole-cell 

configuration 

•Analog control from DAQ for generation of pressure/vacuum 

waveforms 

HSPC headstage attached 

to patch pipette holder 

ALA Scientific’s Pressure-Vacuum Pump (PV-PUMP) is an ideal pressure/vacuum 

source for the HSPC-1. Not only is balanced pressure and vacuum provided, 

but the supporting platform dampens pump oscillations to the headstage, thus 

reducing mechanical noise. Emergency release valves are incorporated and open when 

pressure or vacuum exceed factory limits. 


Max. Input Pressure/Vacuum 

+/-7psi; 362mmHg 

Standard Output Pressure/Vacuum Range 

+/-200mmHg 

Noise 

+/-10mV; +/-1mmHg 

Power 

110/220V AC; 0.5A Slow Blow 

Controller 

2.9lbs/1.32kg - 8.5”/21.6cm x 7.5”/19cm x 4”/10cm 

Headstage 

0.5lbs/0.23kg - 3.75”/9.5cm x 1.75”/4.4cm x 1.75”/4.4cm 

Typical Speed of Response 

0 to 100mmHg jump in 10ms: 0 to 100% settling time 

Command Input 

20mV/mmHg 

Monitor Output 

20mV/mmHg 

Set Point Control (Holding Pressure/Vacuum offset control) +/-200mmHg 

Water Alarm 

Optical liquid detection sensor to protect valve 

HSPC-1 

PV-Pump 


Complete pressure clamp system includes controller, piezo valve headstage and cable, 

misc. tubing, and fittings 

Pressure / vacuum pumps with output tubing 

18

Specialized Electrophysiology 

Optogenetics is becoming an increasingly important research method in the 

neuroscience community. ALA Scientific’s Light Stimulation Device 

(LSD-1), can be incorporated into existing electrophysiology setups 

where light sensitive ion channels are being studied. Optical stimulation over 

standard electrical stimulation techniques affords researchers the advantage 

of non-invasively stimulating targeted areas. 

Key features of the LSD-1 include: 

• LED intensity control via manual knob or via analog signal for 

computer control 

• Easily changeable LED for different wavelength use 

• Low-noise electronics for integration into electrophysiology setups 

• Field illumination when light guide connected to scope optics 

• No warm-up period needed 

ALA LSD-1 and ALA LG shown above 

(sold separately) 

Wavelengths Color 

(nm) 

455 Deep Blue 

496 Verde Green 

532 True Green 

578 Amber 

590 Yellow 

612 Orange/Red 

632 Red 

ALA LSD-1XXX 

ALA LSDLG 

ALA LED-XXX 

Controller 

Power 

Light Guide 


One-channel LED light stimulating device (XXX-specify wavelengths) 

light guide 

interchangeable LED (XXX-specify wavelengths) 


4.6”/11.6cm x 3.6”/90.5cm x 2.25”/57.3cm; 0.8lbs/0.36kg 

5VDC/2A 

300-560nm, 0.12in Core, 1.6in bend radius, 39.4in length 

ALA Scientific’s Carbon Fiber Electrodes (CFE-1) are the ideal electrodes for voltammetric and amperometric 

studies. They are critical for identifying and determining the kinetics of released neurotransmitters. Electrodes are made 

through electrophoretic deposition of paint or parylene coating which creates the highly sensitive environment for the detection 

of these small signals of interest. Electrode holders for Axon amplifiers or with BNC connection available to ensure stable 

connection. 

Key features of CFE-1’s include: 

•5µm carbon fibers for recording from small cells 

•Multiple re-uses due to easy re-cutting of tip 

•Slim design for use with water-immersion objectives 


ALA CFE-1 Carbon Fiber Electrodes - pack of 10 - 5µm fibers 

ALA CFE-H-AXU CFE Electrode Holder for Axon Universal Amplifiers 

ALA CFE-H-BNC CFE Electrode Holder for EPC/BNC Amplifiers 


Size 

60mm x 2mm 

Diameter 5μm 

19

Pipette and Electrode Holders 

ALA Scientific’s line of pipette holders are stable and versatile for the full range of electrophysiology applications. All holders 

are made of low noise polycarbonate and come standard with a Ag wire and accomodate glass OD of 1.5mm. Ag/AgCl 

pellet instead of Ag wire, other glass sizes, as well as different configuration holders like right angle and perfusion ports are 

available. 

ALA Scientific’s BNC type holders are made for use with all HEKA amplifiers, most npi electronic 

amplifiers, Dagan amplifiers, and any other amplifier with a BNC type connector. 

ALA Scientific’s Axon/Molecular Devices type holders are made for use with 

all Axon amplifiers, including Axopatch 1 series, 200A, Axolamp-2A and all Axon amplifier with 

the universal connector input. 

ALA Scientific’s Injection/Ejection holders can be made for use with all amplifiers depending 

on input connector. These holders come with easy manipulator mounting bar and female luer fitting for 

pressure inputs. 


PH _ P _ _ _ _ 

P = Polycarbonate 

I = Injection* 

(only availble in Polycarbonate) 

Glass OD 

1.0mm, 1.2mm, 

1.5mm, 2.0mm 

*custom options available 

P = Change Ag wire 

to Ag/AgCl Pellet 

Side Port Options 

0 = No Side Port 

1 = Standard Patch Perfusion Port 

2 = Additional Side Perfusion Port 

TH = Threaded Side Port 

For Injection Only 

Angle of Headstage Connector 

Input Connector 

0°, 30°, 45°, 90° 

BNC = Heka, Dagan and most npi headstages 

AXU = All Axon universal headstages 

AXP = Axopatch 1 series & 200A patch clamp amplifiers 

AXC = Older Axoclamp amplifiers 

ALA Scientific’s KCL Bridge Electrode holders are ideal for stabilizing electrode potentials 

and improving noise performance. These holders eliminate the need for Cl- in recording 

pipette solutions due to an additional piece which holds a small amount of KCl solution, so a complete 

circuit is then formed between the AgCl pellet and the recording electrode via this bridge. 


PPH-KCL-AXU 

PPH-KCL-AXC 

PPH-KCL-AXP 

PPH-KCL-B 

KCl bridge pipette holder for Axon universal type headstages 

KCl bridge pipette holder for Axon Axoclamp 2A type headstage 

KCl bridge pipette holder for Axon 1D/200A type headstage 

KCl bridge pipette holder for BNC type headstages 

ALA Scientific carries a full line of Ag/AgCl reference pellets. A variety of sizes are available - all embedded 

on a 0.25 mm OD Ag wire. They are reusable, resurfaceable, never need chloriding and are esentially non-polarizable. 


P-BMP-0.8 0.8mm x 3mm Ag-Ag/Cl pellet with 70mm embedded Ag wire 

P-BMP-1 1mm x 2.5mm Ag-Ag/Cl pellet with 70mm embedded Ag wire 

P-BMP-2 2mm x 4mm Ag-Ag/Cl pellet with 10mm embedded Ag wire 

P-BMD-4 1mm x 4mm Ag-Ag/Cl disc pellet with 10mm embedded Ag wire 



20 

Pipette Holder Replacement Parts 

COM O’RINGS-S Replacement silicone O’rings for pipette seals - for all pipette holders - pack of 10 

COM O’RINGS-P Replacement silicone O’rings for pierced Ag wire seal - for all pipette holders with Ag wire - pack of 10 

ENDCAP 

Replacement end cap for pipette holders - specify diameter and holder type 

PPH-BNC-PIN Brass pin insert for BNC holders 

PPHBNCTOOL BNC holder wrench - polycarbon

Pipette Processing and Mounting Systems 

ALA Scientific’s Coating and Polishing Microforge (CPM-2) can 

both fire-polish and Sylgard ® coat glass pipettes. Coating is necessary in order to 

reduce noise from pipette capacitance and dielectric loss. The pressure polishing 

method is also available, as an option, to minimize series resistance with small-tip 

pipettes and to help facilitate whole-cell recording of small cells. The CPM-2 is available 

as a kit to mount onto an existing fixed stage inverted microscope, or available 

as a complete system with the KXD100 scope (see page 23). 

Key features of the CPM-2 include: 

•Pressure polishing option to make small tip low-resistance pipettes 

•Rotating electrode holder for even coating 

•Coating and polishing without electrode removal 

•Convenient control of heat timing with foot pedal switch. 

Options include ALA Scientific’s PR-60 which allows for easy regulation of house 

or tank pressure. The two stage process regulates input pressure so output pressures (up to 60 psi) 

does not fluctuate. Bleed and pressure modes are used to completely de-pressurize pipette when 

changing electrodes. The PR-60 includes a dual scale pressure guage and luer fittings for the inlet 

and outlets. This regulator is ideal when integrating the pressure polishing method. 


Power Output to filaments 15VDC/2.5A 

Controller Dimensions Weight 7.65x9.85x3.85” - 6lbs 0.2 oz (2.82 kg) 

Polishing Filament Ptlr (90%/10%), 0.25mm diameter, 0.4W 

Input Pressure (Min/Max) 12/30 psi (83/200kPa) 

CPM-2 Power Supply 

110/240 VAC to 15VDC/ 3.7A CE compliant 

Polishing Manipulator Travlel 70x40x3mm 

Output Pressure (Min,Max) 0/5 psi (0/34.6 kPa) 


CPM-2 

Coater/Polisher Microforge Kit - for use with inverted microscopes: incudes controller, coating & polishing 

filament, footswitch, and XYZ polishing manipulator 

CPM-2w/scope Coater/Polisher Microforge including KXD100 inverted microscope 

PR-60 

60 psi High Pressure Regulator for use in the Pressure Polishing Method 

CPM-HOTJET Replacement CPM-2 hot air jet filament assembly 

CPM-PTIR Replacement PtIR polishing filament - package of 3 

CPM-XY 

CPM-2 XY stage manipulator for pipette movement 

IPH-THP 

Pressure injection pipette holder for use in the Pressure Polishing Method 

CPM-PPHold Bracket to hold injection pipette holder for use in the Pressure Polishing Method 

ALA Scientific’s Microscope Stage Plate (ALAMSP) is the economic 

solution for creating a mountable space for an upright microscope. Additional 

side plates are available to extend working space, or for longer tools. 

Threaded legs allow for height adjustment to accomodate manipulators and fit 

around standard scopes. The center opening (110mm diameter) accomodates 

the HCMIS and MS-STAGE. 

Key features of the ALAMSP include: 

•Option of either magnetic holder feet or pedestals for mounting onto air table 

•Coated steel plate offers magnetic surface for tool placement around preparation 

•Easy- to-assemble and adaptable to all major microscope manufacturers 

MSP-MAG 

MSP-PED 

MSP-SIDE-MAG 

MSP-SIDE-PED 


Microscope stage platform includes 9” x 14” coated steel plate with opening, 4 magnetic bases, 4 x 10” SS threaded rods 

Microscope stage platform includes 9” x 14” coated steel plate with opening, 4 pedestal bases, 4 x 12” SS threaded rods 

8” x 10” side stage for use with MSP-MAG includes 2 magnetic bases and 2 x 10” SS threaded rods 

8” x 10” side stage for use with MSP-PED includes 2 pedestal bases and 2 x 12” SS threaded rods 

21

MEA Accessories 

ALA Scientific provides a variety of accessories that allow researchers to more easily integrate multi-eletrode 

recording systems into their established research methods and techniques. 

P-PORT3 

MEA-MEMMR 

MEA-MEM 

MEA-INSERT 


MEA-INSERT 

Perfusion insert for all MEA planar arrays - polycarbonate 

MEM-SHEET 3” x 3” mambrane sheet (pack of 10) 

MEA-MEM 

MEA-MEM5 

MEA-MEM-PL 

MEA membrane cover: includes one ring with pre-loaded MEM cover 

MEA-MEM covers (qty: 5), O’rings (qty: 5), and loading tool 

MEA-MEM with perfusion inlet/outlet ports and level lock ports 

MEA-MEM-PL5 MEA-MEM coverswith perfusion inlet/outlet ports and level lock ports (qty: 5), O’rings (qty: 5), 

and loading tool 

MEA-MEM-T Membrane loading tool 

MEA-PPORT2 MEA perfusion ports includes 1 x MPIOH-S, 1 x CHOLD, and MMP - for all MCS MEA 1060 

amplifiers 

MEA-PPORT3 MEA perfusion ports includes 1 x MPIOH-S, 1 x CHOLD, 1 x LL-SHOLD, and MMP - please 

specify amplifier 

MEA-MEMMR Membrane cover for 6-well arrays with triangular-shaped wells 

MEA-MEMMR-5 

MEA-MEMMR5-06 

MMP 

Membrane covers for 6-well arrays with triangular-shaped wells (5-pack) 

Membrane covers for 6-well arrays with circular-shaped openings: 3” x 3” membrane sheets 

(pack of 10), O’rings (qty: 5) and loading tool 

Coated steel mounting plate that fits all MCS MEA-1060 amplifiers 

22

Imaging 

ALA Scientific’s KXD-100 Inverted Microscope is an ergonomically designed 

microscope for bright field and phase contrast observation. Wide-field eyepieces (10x and 

16x) and long working distance objectives (10x, 25x, 40x, and phase contrast 25x) are included 

in this trinocular head microscope. 

Optical System 

Viewing Head 

Eyepieces 

LWD Objective 

Phase cotrast Objective 

Stage 

Filters 

Annular Spot 

Illumination 

Condenser 


Infinity Optical System F=180mm 

Siedentopf Trinocular Head 45° Inclined 

WF10X/20mm with reticle 

WF16X/13mm with reticle 

LWD Plan Achromatic Objectives 10X/0.25 WD=7.9mm 



LWD Plan Phase Contrast Positive Objectives25X/0.40 WD=5.0mm 

Mechanical Stage: 190×235mm, 

Moving Range: 81×87mm 

Blue and Green 

Phase Contrast Annular Spot 25X 

Halogen Lamp 6V 20W, Adjustable Brightness 

Condenser NA: 0.35 (WD45/102mm) 


KXD-100 Inverted Microscope 

ALA Scientific’s Zoom645 Stereoscope is an air-tight, anti-electrostatic microscope 

with .8x-5x zoom magnification. Inclination angles of 45 degrees allow user to comfortably 

zoom in on prep while still achieving optimal viewing. Track stand provides smooth anti-mold 

environment for clean preparations. Post and boom stands available 


Optical System 

Greenough stereoscopic 

Head Observation Trinocular 45°, inclined with C adapter 

Interpupillary Adjustment 52-76mm 

Diopter Adjustment ±5° 

Zoom ratio Zoom645 1:6.3 

Magnification Range 0.8X-5X 

Objective 

1X 

Working Distance 115mm 

Eyepiece 

WF10X/F.N.22 

Field of View 

27.5-4.4mm 

ZOOM-645T-TR 

ZOOM-645T-POST 

ZOOM-645T-BOOM 


Stereo Zoom microscope with track stand 

Stereo Zoom microscope with post stand 

Stereo Zoom microscope with boom stand 

and articulating arm 

ALA Scientific’s DCM310 C-type Microscope Camera is trinocular tube-mountable 

and is compatible with the KXD100 and ZOOM645. Camera frame rates are as fast as 11 

frames/second with a USB2.0 connection. Software included. 


Sensor size 1/2” 

Pixel Size 

3.2μm x 3.2μm 

Sensitivity (v/lux-sec @550nm) 1.5 

Max Resolution (Hardware) 2048 x 1536, 3M pixels 

Full Res. Frame Rate 

Rig Essentials 

ALA Scientific’s full line of accessories covers anything you need to finish setting up your lab. 

4POSTRACK 

BUDRACK 

DESKRACK-1 

4-Post open rack with vented stationary shelf and keyboard/mouse shelf 

Economizer rack with rear door, removable sides, pair of mounting rails, sliding shelf, 

stationary shelf, and wheels. 

24” high instrument rack 

1MMPIN 1mm gold pins - pack of 10 

2MMPIN 2mm gold pins - pack of 10 

BARBTOBARB 

CALIPER 

FLUKE115 

GROUNDKIT 

LUERTOBARB 

MF-LUERSET 

TOOLSET 

WELLER-WESS1 

ALLIG-LEAD 

BANANAPLUGS 

BAN-CABLE 

BNC-3 

BNC-6 

1/16” white nylon barb to barb fittings - 25 pieces 

Digital caliper - 6”/150mm total measurement travel 

Digital Multimeter 

Grounding plate with magnetic base, 10 inputs, 10 banana plugs, 10 alligator clips, 

100’ 18GA wire spool 

Polycarbonate male luer to 1/16” barb - 25 pieces 

Male/Female luer-barb set - 10 pieces 

105 piece tool set with case 

Soldering iron 120V AC only 

Alligator clip lead set - package of 10 assortedcolors 

Set of 5 banana plugs 

Banana to banana test leads - package of 10 assorted colors 

BNC to BNC 3’ RG174 50Ω cable 

BNC to BNC 6’ RG174 50Ω cable 

BNC-ALLIGA BNC to alligator clip test cable - 2’ 

BNCF-BAN 

BNCM-BAN 

BNC-T 

HS-ADAPTER-1 

HS-ADAPTER-2 

HS-ADAPTER-3 

BNC female to double banana plug adapter 

BNC male to double banana plug adapter 

BNC male to female/female T adapter 

SMB to BNC headstage adapter 

BNC to BNC headstage adapter 

TEC HVHS SMC to BNC headstage adapter 

24

Rig Essentials 

Save time and money by choosing from a selection of pre-assembled surgical kits useful in many procedures. These 

kits come complete with their own case. All instruments are made of high strength 410 Stainless Steel which provides 

good corrosion and wear resistance. 

MOUSEKIT RATKIT MOUSEVESSELKIT 

PHYSIOLOGYKIT1 

PHYSIOLOGYKIT2 

MOUSEKIT 

RATKIT 


7 piece mouse dissection kit includes: Dumont #5 forceps, Vannas scissor, Iris forceps, dissecting scissor (10cm straight), 

wire retractor, needle holder, blunt probe (1.0mm diameter), instrument case 

7 piece rat dissection kit includes: Dumont #5 forceps, Vannas scissor, Iris forceps (serrated), super cut Iris scissor (10cm 

straight), alm retractor, needle holder, blunt probe (1.0mm diameter), instrument case 

MOUSEVESSELKIT 7 piece vessel cannulation kit includes: vessel cannulation forceps, Vannas scissor, fine serrated forceps, Dumont #5 forceps, 

alm retractor, backfiller with replacement 200μm ID fibers, instrument case 

PHYSIOLOGYKIT1 7 piece basic physiology kit includes: supercut tenotomy scissors (curved), rongeur (3mm jaw), utility scissor, flat jaw 

tweezer, operating scissor (straight sharp/curved blunt), Dumont #5 tweezers, blunt probe (1mm diameter), instrument 

case 

PHYSIOLOGYKIT2 11 piece deluxe physiology kit includes: Vannas scissor, supercut Iris scissor (straight), rongeur (1.3mm jaw), utility scissor, 

operating scissor (straight sharp/blunt curved), Dumont #5 tweezers, blunt probe (1.0mm diameter), Stevenson retractor, 

Iris forceps (curved), Adson forceps (1 x 2 teeth), Olsen-Hegar needle holder, instrument case 

25

60 Marine Street 

Farmingdale, New York 11735 

(631) 393-6401 

sales@alascience.com 

www.alascience.com 

Local Distributor:

Product Catalog - ALA Scientific Instruments

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