Plasmid

lifes.nchu.edu.tw

Plasmid

2011/12/2

Identifying bacteria that have taken up

genetically altered plasmids (1)

11

Sites of cleavage

Fragment

1

Fragment

2

Fragment

3

Fragment

4

1 Cut with a restriction

enzyme

1

DNA from plant

cells is cut into

multiple

fragments with a

restriction

enzyme. (Only a

small part of one

chromosome is

shown.)

Fig. 15-5, p. 298

Identifying bacteria that have taken up

genetically altered plasmids (2)

12

Fragment

1

Fragment

2

Fragment

3

Fragment

4

Produce

recombinant

DNA

Gene for

resistance

to antibiotic

2 2

2 2 2 Recombinant

plasmids are formed

by cutting plasmids

with the same

restriction enzyme,

mixing the plasmids

with the segments of

plant DNA, and

treating with ligase.

Fig. 15-5, p. 298

6

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