PDF catalog - Teknolab AS

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SUPPORTED LIQUID EXTRACTION Figure 1. Comparison of analyte recovery by ISOLUTE SLE+ and LLE Transferable Methods The water immiscible extraction solvents used in LLE are the same for ISOLUTE SLE+. Sample pre-treatment conditions are also the same, meaning existing LLE methods are easily transferable to ISOLUTE SLE+, reducing method development. SLE in 96-well Plate Format The ISOLUTE SLE+ plate consists of 96 extraction wells each containing a modified form of diatomaceous earth. When the aqueous biological fluid sample is applied, it spreads over the surface of the support, and is absorbed. The analytes remain on the surface of the support forming the interface for the extraction; equivalent to the phase interface in LLE. When the water immiscible extraction solvent is applied, analytes are efficiently desorbed, and the solvent is collected. This process is shown schematically in figure 2. Figure 2. Schematic of Supported Liquid Extraction Process 91
SUPPORTED LIQUID EXTRACTION The 96 well-plate format is optimized for the simultaneous extraction of samples (up to 200 µL of plasma or urine per well), using a generic methodology* for neutral, acidic or basic compounds. The plate is processed using a vacuum manifold (VacMaster-96) or automated liquid handling system with vacuum capability. Sample is applied to the plate under vacuum. The aqueous portion is absorbed (unlike SPE, sample does NOT flow through the plate). A water immiscible solvent is then applied to the plate, desorbing the analytes of interest, which are collected in a collection plate. Extracts can then be evaporated and reconstituted in a solvent suitable for analysis (generally by LC-MS). Two ISOLUTE SLE+ plates are available. ISOLUTE SLE+ 200 allows a total aqueous sample load of up to 200µL; ISOLUTE SLE+ 400 allows aqueous sample load of 200 – 400 µL. * A generic procedure for supported liquid extraction of biological fluids using the ISOLUTE SLE+ plate is included with every plate. Ordering Information Description Quantity Part number ISOLUTE SLE+ 200 Supported Liquid Extraction Plate 1 820-0200-P01 ISOLUTE SLE+ 400 Supported Liquid Extraction Plate 1 820-0400-P01 See page 115 and 155 for more details Support Documents ISOLUTE SLE+ User Guide see www.biotage.com 92
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Analytical Sample Preparation CATAL
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CONTENTS CUSTOMER SUPPORT ... 6 PRO
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Customer Support 1-Point Support, T
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CONTACT 1-POINT SUPPORT Contact Bio
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PRODUCT QUALITY Biotage is committe
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PRODUCT SELECTION GUIDE To help you
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PRODUCT SELECTION GUIDE Select a SP
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O SAMPLE PREP FORMAT OPTIONS 96-wel
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O SAMPLE PREP FORMAT OPTIONS True-t
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EVOLUTE Sample Preparation Products
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EVOLUTE ADVANCED POLYMERIC SORBENTS
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NON-POLAR SORBENTS Isolate analytes
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NON-POLAR SORBENTS ISOLUTE non-pola
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ISOLUTE ENV+ HYDROXYLATED POLYSTYRE
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ISOLUTE C18 OCTADECYL (NON-ENDCAPPE
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ISOLUTE MFC18 OCTADECYL (NON-ENDCAP
Page 41 and 42: ISOLUTE C8(EC) OCTYL (ENDCAPPED) Ap
Page 43 and 44: ISOLUTE C4 BUTYL (NON-ENDCAPPED) Ap
Page 45 and 46: ISOLUTE C2(EC) ETHYL (ENDCAPPED) Ap
Page 47 and 48: ISOLUTE PH PHENYL (NON-ENDCAPPED) A
Page 49 and 50: ISOLUTE CN CYANOPROPYL (NON-ENDCAPP
Page 51 and 52: MIXED-MODE SORBENTS Isolate acidic
Page 53 and 54: MIXED-MODE SORBENTS Mixed-mode Sorb
Page 55 and 56: ISOLUTE HCX OCTYL AND SULFONIC ACID
Page 57 and 58: ISOLUTE HCX-5 BUTYL AND SULFONIC AC
Page 59 and 60: ISOLUTE HAX OCTYL AND QUATERNARY AM
Page 61 and 62: EXCH ION EXCHANGE SORBENTS Isolate
Page 63 and 64: EXCH ION EXCHANGE SORBENTS ISOLUTE
Page 65 and 66: ISOLUTE NH2 AMINOPROPYL (NON-ENDCAP
Page 67 and 68: ISOLUTE SAX QUATERNARY AMINE (NON-E
Page 69 and 70: ISOLUTE CBA PROPYLCARBOXYLIC ACID (
Page 71 and 72: ISOLUTE SCX-2 PROPYLSULFONIC ACID (
Page 73 and 74: SO POLAR SORBENTS Isolate analytes
Page 75 and 76: SO POLAR SORBENTS ISOLUTE FL ISOLUT
Page 77 and 78: ISOLUTE SI SILICA Applications Si O
Page 79 and 80: ISOLUTE FL FLORISIL ® PR GRADE Flo
Page 82 and 83: ISOLUTE Bulk SPE Sorbents
Page 84: ISOLUTE BULK SORBENTS ISOLUTE HM-N,
Page 87 and 88: P ISOLUTE PPT+ ISOLUTE PPT+ Protein
Page 90 and 91: Supported Liquid Extraction High Pu
Page 94: SUPPORTED LIQUID EXTRACTION ISOLUTE
Page 97 and 98: ACCESSORIES FOR SPE AND FILTRATION
Page 104 and 105: Sample Processing Products
Page 106 and 107: VACMASTER -10 AND -20 SAMPLE PROCES
Page 108 and 109: VACMASTER-96 SAMPLE PROCESSING MANI
Page 110 and 111: SAMPLE PROCESSING ACCESSORIES VacMa
Page 112: DRY SPE DRY 96 AND 96 DUAL SAMPLE C
Page 115 and 116: BIOANALYTICAL PRODUCTS FOR BIOANALY
Page 122 and 123: Products for Forensic and Clinical
Page 124 and 125: FORENSIC AND CLINICAL PRODUCTS FOR
Page 126: FORENSIC AND CLINICAL PRODUCTS FOR
Page 129 and 130: ENVIRONMENTAL PRODUCTS FOR ENVIRONM
Page 136 and 137: Products for Agrochemical and Food
Page 138 and 139: AGROCHEMICAL AND FOOD ISOLUTE Produ
Page 140: AGROCHEMICAL AND FOOD ISOLUTE Sodiu
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TO QUICKSTART GUIDE TO SPE Introduc
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TO QUICKSTART GUIDE TO SPE Selectiv
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TO QUICKSTART GUIDE TO SPE 1 2/3 4
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TO QUICKSTART GUIDE TO SPE Step 6:
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TO QUICKSTART GUIDE TO SPE Table 4
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TO QUICKSTART GUIDE TO SPE Appendix
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SOLUTE QUICKSTART GUIDE TO SPE 6. A
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Custom Manufacturing Service
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M CUSTOM MANUFACTURING SERVICE High
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Analyte Matrix Application Note Col
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I Ion-exchange sorbents see individ
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Part Number Index Part Number Page
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Part Number Page 902-0013-H 54, 123
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PDF catalog - Teknolab AS

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