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Bacterial Abundance

Bacterial Abundance

Dilution Plates 1 ml 1

Dilution Plates 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 9 ml 10 0 10 -1 10 -2 10 -3 10 -4 10 -5 10 -6 Statistically relevant colony density: 30 - 300 Technique largely used for isolation or water testing, such as coliform test.

Dilution Plate Calculations N: Number of colonies on plate V S : Volume pipetted onto Petri plate. D: Dilution factor for test tube plated out. ρ: Concentration of cells in original sample (cells ml -1 ) ρ = N V S 1 D Example: N: 33 V S : 100 µl D: 10 -4 33 1 ρ = 0.110 6 −1 = 3.3× 10 cells ml -4

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