Analysis of Cr (VI) in Trivalent Chromate Coating Film with UH5300

Analysis of Cr (VI) in Trivalent Chromate Coating Film with UH5300
UH5300
INTRODUCTION
The standard installation of UH5300 spectrophotometer includes an automatic 6-cell turret. When analyzing many
samples, the increased work efficiency can be expected by using the “intelligent Start” function. UH5300
spectrophotometer also focuses on the absorbance differences between cells. All cells are measured for the auto-zero
values, which are recorded as the correction values. Therefore, the instrument is capable of handling the analysis where
a slight absorbance difference is being measured. This time, Cr(VI) in water samples was analyzed by using this function.
As a result of the analysis, a calibration curve with a high correlation coefficient (R 2 = 0.9999) was obtained.
Analyte
Analysis method
METHOD
: Cr(VI)
: Diphenylcarbazide absorptiometry
(Simple analysis by using a water quality test kit
of Kyoritsu Chemical Check Lab., Corp.)
Reagent : Water quality test kit No.31 Cr 6+ ,
Kyoritsu Chemical Check Lab., Corp.
Range of quantitative analysis : 0.02 - 1.0 mg/L
Refer to JIS K0102 65.2 for the official method
Instrument
: UH5300
ANALYTICAL CONDITIONS
Slit
: 1 nm
Sample
Stir
PREPARATION
25 mL
Add reaction reagent R-1 (*1)
Let stand for 5 min
Solution for measurement
Temperature: Room temperature
*1 Kyoritsu Chemical Check Lab., Corp.
Reagent Set for Water Analyzer No.31 Cr 6+
Scan speed
: 400 nm/min
Measurement wavelength
: 542 nm
** Contact Kyoritsu Chemical Check Lab.
Corp. for the information including the
effects of the interfering substances
and the details of the reagents.
High throughput measurement by “intelligent Start” function
Open the sample
chamber
Set the sample
Set the cell in the designated
position.
6 cells can be used.
Close the sample
chamber
When the lid of the sample
chamber is closed, the sample
measurement will start
automatically.
Completion of
Start of Measurement
measurement
• When the sample cell is set and the sample chamber is closed, the measurement will start
automatically. (Patent pending: Patent application No. 2012-181302)
• The starting operation after switching the sample cells is omitted.
KEY WORDS
Electronics/Semiconductor Related, Other Electronics/Semiconductor Related,
Environmental Chemistry, Environment, Cr(VI), Absorption Spectrum,
Calibration Curve, Coloring Reagent, Hexavalent Chromium, UV, UH5300,
U-5100, U-2900,U-2910
Spectrophotometer
(UV)
Sheet No.
UV120005-01
Hitachi High-Technologies Corporation

Analysis of Cr(VI) in Trivalent Chromate Coating Film with UH5300
UH5300
0.8
0.8
0.6
542 nm
1 mg/L
0.6
0.5 mg/L
Abs
0.4
Abs
0.4
0.2 mg/L
0.1 mg/L
0.2
0.2
0.02 mg/L
0.0
0.0 0.2 0.4 0.6 0.8 1.0
Concentration (mg/L)
Calibration Curve of Cr (VI)
R 2 : 0.9999
0.0
400 500 600 700
Wavelength (nm)
Absorption Spectrum of Cr (VI)
Blank
Addition Recovery Test for Extract
Extract Extract + 0.5 mg/L Recovery rate
0.16 ± 0.002 0.69 ± 0.003 105.6 ± 0.6 %
KEY WORDS
Spectrophotometer
Electronics/Semiconductor Related, Other Electronics/Semiconductor Related,
(UV)
Environmental Chemistry, Environment, Cr(VI), Absorption Spectrum,
Calibration Curve, Coloring Reagent, Hexavalent Chromium, UV, UH5300, Sheet No. UV120005-02
U-5100, U-2900, U-2910
Hitachi High-Technologies Corporation