RNA FOLDING DURING TRANSCRIPTION

faculty.biol.ttu.edu

RNA FOLDING DURING TRANSCRIPTION

These two examples suggest that there are similarities

between in vivo and in vitro folding.

However, there are studies that show that RNA folding

can be different in vitro and in vivo, , as exemplified by

the human telomerase RNA. The formation of the

phylogenetically conserved pseudoknot in the 5′ 5 part of

this RNA is not observed in vivo.

Furthermore, exon mutants of the Tetrahymena pre-

rRNA show a 100-fold decrease in self-splicing

splicing in vitro,

but were fully rescued in vivo when the pre-rRNA

rRNA was

expressed in Escherichia coli, , suggesting that RNA

folding is facilitated in vivo

More magazines by this user
Similar magazines