Modular microfluidics platform for POC system prototyping ... - CiPoC
Modular microfluidics platform for POC system prototyping ... - CiPoC
Modular microfluidics platform for POC system prototyping ... - CiPoC
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<strong>Modular</strong> <strong>microfluidics</strong> <strong>plat<strong>for</strong>m</strong> <strong>for</strong> <strong>POC</strong><br />
<strong>system</strong> <strong>prototyping</strong> and cell culturing<br />
Martin Dufva<br />
<br />
DTU Nanotech<br />
Technical University of Denmark<br />
martin.dufva@nanotech.dtu.dk
Do you have this in your lab<br />
3 DTU Nanotek, Danmarks Tekniske Universitet
Inspirations <strong>for</strong> <strong>plat<strong>for</strong>m</strong> design<br />
Standardized interface<br />
<strong>Modular</strong><br />
Highly integrated<br />
4 DTU Nanotek, Danmarks Tekniske Universitet
PoC <strong>prototyping</strong><br />
Materials<br />
Ability to use materials similar to those in final PoC device<br />
<br />
Per<strong>for</strong>mance<br />
Ability to drive 1 (simulating final PoC device) or up to 32 parallel assays<br />
(used in assay development)<br />
<br />
Usability<br />
Systems should be compatible with natural workflows<br />
<br />
<br />
Efficacy<br />
Minimize <strong>system</strong>/application specific development<br />
<br />
<br />
<br />
5 DTU Nanotek, Danmarks Tekniske Universitet
Connect hard chips to microfluidic ribbons<br />
Si/polymer hybrids Silicon Polymer<br />
6 DTU Nanotek, Danmarks Tekniske Universitet
Micropump per<strong>for</strong>mance<br />
Channel-to-channel (% CV): 6%<br />
Durability: >33 ml per channel<br />
Two-directional<br />
A few nL-80 microL/Min<br />
Pulsating flow<br />
No practical consequence<br />
<strong>for</strong> mixing and liquid<br />
switching operations on<br />
chip as compared with<br />
syringe pumps.<br />
1<br />
SKAFTE-PEDERSEN, P.*, SABOURIN, D.*, ET AL., MULTI-CHANNEL PERISTALTIC PUMP FOR MICROFLUIDIC<br />
APPLICATIONS FEATURING MONOLITHIC PDMS INLAY, LAB CHIP, VOL. 9, 2009.<br />
7 DTU Nanotek, Danmarks Tekniske Universitet
Valve<br />
8 DTU Nanotek, Danmarks Tekniske Universitet
Valve in action
Reagent vials integration<br />
10 DTU Nanotek, Danmarks Tekniske Universitet
Compatible with work flow and equipment<br />
11 DTU Nanotek, Danmarks Tekniske Universitet
Measurements of dopamine release in living<br />
neurones using a highly integrated <strong>system</strong><br />
400<br />
350<br />
300<br />
)<br />
A250<br />
(p<br />
t 200<br />
r<br />
e<br />
n<br />
150<br />
u<br />
C100<br />
50<br />
0<br />
-50<br />
-100<br />
PC12 cells<br />
Clean electrode<br />
0 20 40 60<br />
Time (sec)<br />
Electrochemical sensing integration:<br />
Prof Jenny Emneus, DTU Nanotech<br />
12 DTU Nanotek, Danmarks Tekniske Universitet
Microarray based genotyping<br />
13 DTU Nanotek, Danmarks Tekniske Universitet
Automated in situ hybridization<br />
Controller<br />
HistoFlex<br />
Tubing<br />
Connector<br />
Motor<br />
Merging<br />
Chip<br />
Valve<br />
Micro<br />
pump<br />
Waste<br />
Vial Rack<br />
Motor<br />
10 cm<br />
Together with Kim Holmstrøm, Bioneer A/S<br />
14 DTU Nanotek, Danmarks Tekniske Universitet<br />
7-6-2011
Online monitoring of pesticides<br />
Measure pesticides using an immunoassay with electrochemical detection<br />
Small enough to be put into a well (<strong>for</strong> onsite simulation)<br />
Responsible: Associate Professor Mogens Havsteen DTU Nanotech<br />
15 DTU Nanotek, Danmarks Tekniske Universitet
System stress test (cell culturing)<br />
MainSTREAM 1.0<br />
Chip and Syringe pump<br />
Setup<br />
Time<br />
Sites<br />
(No.)<br />
Culture<br />
Days<br />
Failures<br />
(%)<br />
Bubbles<br />
(%)<br />
Parts<br />
(No.)<br />
(h)<br />
16 DTU Nanotek, Danmarks Tekniske Universitet<br />
7-6-2011
MainSTREAM develoment<br />
MainSTREAM 1.0<br />
8-channel pumps
Acknowledgements<br />
MainSTREAM core components<br />
and <strong>system</strong>s design<br />
David Sabourin<br />
Peder Skafte-Pedersen<br />
Massimo Alberti<br />
Alvaro José Conde<br />
Maciek Skolimowski<br />
Stem cell biology<br />
Mette Hemmingsen<br />
Philippe Collas (Uni Oslo)<br />
Alberto Martínez-Serrano<br />
(Uni Madrid)<br />
DNA microarrays<br />
David Sabourin<br />
Sun Yi, Anders Wolff<br />
Jesper Pedersen (Uni Copenhagen)<br />
In situ hybridization<br />
Martin J Søe (Bioneer A/S)<br />
Kim Holmstrøm (Bioneer A/S)<br />
Electrochemistry on cells<br />
Arto Heiskanen, Vasile Coman<br />
Jenny Emneus, EU – FP7 consortia<br />
Tissue slices cultures<br />
Arto Heiskanen, Jenny Emneus<br />
Indumathi Vedarethinam<br />
Merab Kokaia (Lund University)<br />
Biocompatibility<br />
Joanna Lopacinska<br />
Theory and simulation<br />
Peder Skafte-Pedersen<br />
Søren Vedel, Henrik Bruus<br />
Circle to circle amplification<br />
Monica Brivio, Annika Alh<strong>for</strong>d<br />
Mats Nilsson<br />
Water quality control<br />
Mogens Havsteen Jacobsen<br />
Basil Uthuppu<br />
18 DTU Nanotek, Danmarks Tekniske Universitet<br />
Dufva LOECEC 2011
Literature<br />
David Sabourin, Peder Skafte-Pedersen, Martin Jensen Søe, Mette Hemmingsen, Massimo Alberti, Vasile Coman, Jesper<br />
Petersen, Jenny Emnéus, Jörg P. Kutter, Detlef Snakenborg, Flemming Jørgensen, Christian Clausen, Kim Holmstrøm,<br />
and Martin Dufva. The MainSTREAM Component Plat<strong>for</strong>m: A Holistic Approach to Microfluidic System Design. Journal of<br />
Laboratory 2012 as doi:10.1177/2211068212461445.<br />
Vergani, M, Carminati, M , Ferrari, G, Landini, E, Caviglia, C, Heiskanen, A, Zor, K, Sabourin, D, Dufva, M, Raiteri, R,<br />
Emnéus, J and Sampietro, M. Multichannel Bipotentiostat Integrated with a Microfluidic Plat<strong>for</strong>m <strong>for</strong> Electrochemical<br />
Real-Time Monitoring of Cell Cultures. 2012 accepted, IEEE Transactions on Biomedical Circuits and Systems journal.<br />
Skafte-Pedersen P, Hemmingsen M, Sabourin D, Blaga F, Bruus H, Dufva M. A self-contained, programmable<br />
microfluidic cell culture <strong>system</strong> with real-time microscopy access. Biomedical microdevices (2012), 14(2), 385-399.<br />
Søe M, Sabourin D, Alberti M, Holmstrøm K, Dufva M. HistoFlex – a microfluidic device providing uni<strong>for</strong>m flow<br />
conditions enabling highly sensitive, reproducible and quantitative in situ hybridizations. Lab on a chip journal 2011.<br />
Sabourin D, Petersen J, Dufva M. 2010. Microfluidic DNA microarrays in PMMA chips: streamlined fabrication via<br />
simultaneous DNA immobilisation and bonding activation by brief UV exposure. Biomed Microdevices. 2010 Aug;12(4):<br />
673-81.<br />
Sabourin D, Snakenborg, D and Dufva M. 2010, Interconnection blocks with minimal dead volumes permitting planar<br />
interconnection to thin microfluidic devices, Microfluidics and Nanofluidics. 9(1):87-93<br />
D Sabourin , M Dufva , T Jensen , J Kutter and D Snakenborg. et al. 2010, One-step fabrication of microfluidic chips<br />
with in-plane, adhesive-free interconnections. J. Micromech. Microeng. 20 (3): 037001.<br />
Sabourin D, Snakenborg D, Dufva M. Interconnection blocks: a method <strong>for</strong> providing reusable, rapid, multiple, aligned<br />
and planar microfluidic interconnections. Journal of micromechanics and microengineering. 2009;19:035021.<br />
Skafte-Pedersen, P., D. Sabourin, M. Dufva, and D. Snakenborg. 2009. Multi-channel peristaltic pump <strong>for</strong> microfluidic<br />
applications featuring monolithic PDMS inlay. Lab on a chip 9:3003-3006..<br />
<br />
19 DTU Nanotek, Danmarks Tekniske Universitet
PoC <strong>prototyping</strong><br />
Usability, interference<br />
Instrumentation<br />
development<br />
Input to hardware<br />
development<br />
Test bed<br />
Input to assay<br />
design<br />
Assay<br />
development<br />
<strong>POC</strong> device<br />
User experience<br />
Clinical tests<br />
2 DTU Nanotek, Danmarks Tekniske Universitet
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