FCS - Fluorescence Correlation Spectroscopy - FMP

Other common methods to use fluorescence fluctuations to probe molecular
interactions include Photon Counting Histograms (PCH).
Possible applications
The measurement of translational diffusion is probably the simplest measurement
which can be performed with FCS. The most frequent application of FCS consists in
assaying molecular interactions (binding/debinding). The method is best suited to
study the binding of a small compound (e.g. a ligand) to a bigger molecular object
(e.g. the ligand’s receptor). By the use of cross-correlation protein-protein interactions
(e.g. receptor dimerization) can be examined.
When an appropriate model is known, FCS can be applied to obtain quantitative
information such as:
• diffusion coefficients,
• hydrodynamic radii,
• average concentrations,
• kinetic chemical reaction rates,
• singlet-triplet dynamics.
Examples of applications in our group
Schmid, A. et al., Traffic 10 2-15 (2009)
Schmidt V. et al., EMBO J. 31 187-200 (2011)
Ridelis I. et al., FEBS Lett. 586 784-791 (2012)
Teichmann A. et al., J Biol Chem. 287 27265-27274 (2012)