Mycoplasma pneumoniae Chlamydophila ... - Diagenode Diagnostics

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Mycoplasma pneumoniae Chlamydophila ... - Diagenode Diagnostics

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DIAGENODE MYCOPLASMA PNEUMONIAE / CHLAMYDOPHILA PNEUMONIAE REAL-TIME PCR USER MANUAL

• Virus control extraction must be manipulated using a safety cabinet for a manual extraction

procedure or in a specific area for automated extraction procedures; as suggested by the

manufacturer.

Contamination

• Perform nucleic acid release, isolation and real time amplification in separate laboratory

areas.

• Keep all tubes and vials closed when not in use.

• The PCR laboratory (safety cabinet, DNA/RNA extraction platform, bench coat, etc.) must

be cleaned after each PCR experiment with appropriate solutions.

• The contaminated kits must be thrown in a specific waste bin for biological substances.

• The kit should not be used by somebody displaying symptoms of the disease being detected

by this kit.

• The positive and negative PCR control must be performed with each reaction of PCR.

• Gloves must be worn.

• Aerosol resistant tips must be used for all PCR mixtures.

• Tubes must be harvested before opening.

• Do not pipette any of the materials by mouth. Do not smoke, eat or drink in areas which

specimens or kit reagents are handled.

• The experiment should not be carried out during pregnancy due to risks to the newborn

baby.

CAUTION

Storage of the kit at room temperature can lead to the degradation of the primers / probes /

positive control . This will lead to a decreased sensitivity and we would strongly advise a new kit .

Material Required, Not Provided

• Pipettes

• Sterile pipette tips with filters

• Powder-free gloves

• Vortex mixer

• Desktop centrifuge

• RNase/Dnase-free microcentrifuge tubes (1,5 and/or 2 ml)

• RNase/DNase free water

• DNA isolation kit (see point II. Sample Extraction/DNA Isolation)

• Consumables and accessories (reaction tubes, microcentrifuge, reaction plates, adhesive

seal) for an ABI / Roche / Biorad / Qiagen / Stratagene or Cepheid system

• Master mix (see point III. PCR Reaction Set-up)

• Real time PCR instrument: ABI / Roche / Biorad / Qiagen / Stratagene or Cepheid.

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