Significance, Detection and Elimination of ... - Minerva Biolabs
Significance, Detection and Elimination of ... - Minerva Biolabs
Significance, Detection and Elimination of ... - Minerva Biolabs
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Importance <strong>of</strong> the Mycoplasma Tests<br />
• Cell cultures <strong>of</strong>fer ideal living conditions to parasitic mycoplasma:<br />
contamination is possible at any time<br />
• Despite titers from 10 7 to 10 8 mycoplasma/ml in cell cultures, no<br />
apparent projection referring to the contaminating mycoplasma<br />
species <strong>and</strong> the cell type<br />
• Microscopically i unrecognizable<br />
• St<strong>and</strong>ard antibiotics can allow contamination levels lower than<br />
detection levels, Pen/Strep does not provide protection from<br />
contamination<br />
!! only each 10th cell culture user regularly tests<br />
for mycoplasma contamination !!<br />
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