Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs

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Sources of Contamination (continued) • The User • direct entry while handling, usually from the oral flora • droplet transfer • lacking disinfection • careless technical work From:Toni Lindl, Zell-und Gewebekultur
Importance of the Mycoplasma Tests • Cell cultures offer ideal living conditions to parasitic mycoplasma: contamination is possible at any time • Despite titers from 10 7 to 10 8 mycoplasma/ml in cell cultures, no apparent projection referring to the contaminating mycoplasma species and the cell type • Microscopically unrecognizable • Standard antibiotics can allow contamination levels lower than detection levels, Pen/Strep does not provide protection from contamination !! only each 10th cell culture user regularly tests for mycoplasma contamination !!
Page 1 and 2: Mycoplasma Contaminations in the Ce
Page 3 and 4: Mycoplasma • Found in1898 classif
Page 5 and 6: Mycoplasma (continued) • Parasite
Page 7 and 8: Mycoplasma in the Electron Microsco
Page 9 and 10: Effects on Cell Cultures (continued
Page 11: Sources of Contamination • Primar
Page 15 and 16: Fluorescence method • Simple, dir
Page 17 and 18: NAT method • Nucleic acid amplica
Page 19 and 20: Features of Venor ® GeM • Valida
Page 21 and 22: Frequency of Testing • New cell a
Page 23 and 24: Mycoplasma Elimination Methods •
Page 25 and 26: Effect of Mynox ® on Mycoplasma El

Mycoplasma Contaminations in the Cell Culture ... - Minerva Biolabs

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