Trisha Towandatrisha_towanda@yahoo.comEducationThe Evergreen State College, Olympia, WAMaster of Environmental Studies program, 2006-presentBachelor of Science, Graduation in 2005, Focus: Marine ScienceFront Range Community College, Fort Collins, COFirst College Semester, 2001Relevant Work ExperienceResearch TechnicianWashington State Department of Natural Resources2006-January, 2008Recorded and analyzed video recordings of underwater transects of Zostera marina in nearshoreenvironments to quantify total eelgrass habitat in Puget Sound. Participated in weeklong researchcruises in Puget Sound. Supervisor: Dr. Jeff GaeckleResearch AssistantNSF grant OCE-9986680 (Ecophysiology of gelatinous zooplankton in hypoxic estuaries) at TheEvergreen State College2002-2005Provided general laboratory support for the project by maintaining stocks of filtered seawater,chemicals, equipment and instruments. Responsible for constructing and maintaining planktonkreisels.Reared Artemia and maintained gelatinous zooplankton. Presented scientific talks and posters atnational and international marine science conferences. Supervisor: Dr. Erik V. Thuesen.Key Projects:• Primary author in an investigation of symbiotic relationships between Phacellophora camtschatica,Cancer gracilis and Hyperia medusarum. Included field and behavioral observations, Nomarski,SEM and dissection photomicrography, stable isotope comparisons, measurement of oxygenconsumption rates and enzyme analyses.• Contributing author in an investigation into the effects of hypoxia on the physiology and behavior ofAurelia labiata. Monitored salinity, temperature and oxygen saturation with a YSI meter atsampling stations in southern Puget Sound. Collected specimens for experimentation. Conductedlaboratory experiments on the swimming patterns of A. labiata in various oxygen concentrations.Teaching AssistantThe Evergreen State College2003-2005, 2007Prepared laboratory experiments for undergraduate biology programs. Assisted students withindividual projects. Instructed and certified students in the use of instruments and microscopes.Collected and maintained organisms in aquaria. Supported faculty in the organization andimplementation of field trips (3-5 days) including tide-pooling, snorkeling and overnight backcountrystays. Assisted with laboratory final exam set-up and administration. Supervisor: Dr. Erik V. ThuesenTaxonomistPacific Shellfish Institute2004-2007Identified interstitial crustaceans from sediment samples for research projects funded by theWashington State Department of Ecology, investigating community effects of pesticides to controlghost shrimp. Developed a photomicrograph key to the most commonly encountered species in thestudy. Organized data for analysis. Supervisor: Dr. Steven Booth.
Trisha Towanda 2Research AssistantOceanographic cruise for The Census of Marine LifeSeptember, 2005, RV Point SurSecured instruments to prevent damage and facilitate use while at sea. Assisted in thedeployment and retrieval of the Tucker trawl plankton net. Sorted specimens to taxonomic group.Prepared formalin solutions for the preservation of voucher specimens. Counted and weighed voucherspecimens after the cruise. Organized data for analysis.Supervisor: Dr. Erik V. Thuesen.Scientific ProficiencyEquipment:YSI O/T/S meterOsmometerFiltration systemsOxygen sensing optodeVan Dorn BottlesPlankton netsOtter trawlSecchi DiskDichotomous keysLight microscopes: Nomarski universal,phase contrast, dissectionScanning electron microscopeSyncroscopy Auto-Montage systemPressurized gassesSpectrophotometer and Ocean Optics softwareDiode array with DOS softwareSpectroscopic instruments (FT-NMR, GC-MS)pH meterSDS PAGE equipmentAseptic tools and techniquesCulturing tools and techniquesSmall boat operationRecreational SCUBABasic lab equipment: balances, centrifuge,autoclave, etc.Procedures: As a student and teaching assistant, I prepared labs that demonstrated concepts andtechniques including the following:Stratification demonstration and experimentationMicroscopy and slide preparation labsChlorophyll extraction and quantification with spectrophotometric analysisEnzyme activity analysesMeasurement of metabolic respiration of algal-invertebrate symbiontsOsmoregulationParasitologyInvertebrate and vertebrate dissectionMicrobial isolation and cultureBiodiversity studiesDehydration and SEM preparationSeed germination in agar slants for inoculation with nitrogen-fixing bacteriaI also prepared field-based labs that required small boat deployment and operation, wilderness trippreparation, snorkeling, and the assemblage and maintenance of equipment and instruments forbiodiversity, taxonomy, and water quality sampling in the field.Software: Comfortable with PC and Mac platforms. Proficient with Adobe Photoshop and Illustrator;Microsoft Word, Excel, and PowerPoint; KaleidaGraph; EndNote and ProCite; Latitude Lotusapplications.PublicationsThuesen, E.V., L.D. Rutherford, P.L. Brommer, K. Garrison, M.A. Gutowska & T. Towanda, 2005.Intragel oxygen promotes hypoxia tolerance of scyphomedusae. Journal of Experimental Biology,208:2475-2482.
Trisha Towanda 3Towanda, T. & E.V. Thuesen, 2006. Ectosymbiotic behavior of Cancer gracilis and its trophicrelationships with its host Phacellophora camtschatica and the parasitoid Hyperia medusarum. MarineEcology Progress Series, 315:221-236.Towanda, T & Falxa, Greg. In prep. Emergence and foraging patterns of Myotis spp. bats in thesouthern Puget Sound region.PresentationsTowanda, T, M.T. Biondi & E.V. Thuesen (2002). Symbiotic associations of Cancer gracilis(Brachyura) and Hyperia medusarum (Amphipoda) with Phacellophora camtschatica (Scyphozoa) inthe Puget Sound estuary. Western Society of Naturalists Annual Meeting, Monterey, California,November 2002. Poster.Towanda, T. & E.V. Thuesen (2003) The symbiotic association of the graceful crab (Cancer gracilis)and the fried egg jellyfish (Phacellophora camtschatica) in Puget Sound. Western Society ofNaturalists Annual Meeting, Long Beach, California, November 2003. Oral presentation.Gutowska, M.A., T. Towanda & Erik V. Thuesen (2004). Physiological and behavioral responses ofthe jellyfish Aurelia labiata to hypoxia stress. American Association for the Advancement of ScienceAnnual Meeting, Seattle, Washington. February 2004. Poster.Towanda, T. & E.V. Thuesen (2004) The symbiotic association of the graceful crab (Cancer gracilis)and the fried egg jellyfish (Phacellophora camtschatica) in Puget Sound. American Society ofLimnology and Oceanography Annual Meeting, Honolulu, Hawaii, February 2004. Oral presentation.Towanda, T. & Falxa, Greg (2007) Emergence and foraging patterns of Myotis spp. bats in thesouthern Puget Sound region. The Society of Northwestern Vertebrate Biology Joint Annual Meetingin conjunction with Northwest Scientific Association and Northwest Lichenologists. Victoria, BritishColumbia, February 2007. PosterHonors & AwardsEvergreen State College Undergraduate RepresentativeWashington State Legislature Research DaysMarch 2005Contributor to the Puget Sound Partnership Science Working GroupOctober 2006Evergreen State College Studebaker FellowshipFebruary 2007Evergreen State College Foundation GrantFebruary 2008