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BDâ„¢ P100 Blood Collection Kit

BDâ„¢ P100 Blood Collection Kit

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BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong><br />

Setting the Standard in Plasma Proteomics<br />

Explore


Minimize Preanalytical Variability<br />

with BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong><br />

Human plasma contains a wide variety of proteins<br />

that hold potential as biomarkers for proteomics-based<br />

diagnostic and prognostic assays. * These proteins are<br />

subject to proteolytic degradation and modification<br />

during and after blood collection. The use of protease<br />

inhibitors – mixed immediately with blood – minimizes<br />

preanalytical variability associated with plasma sample<br />

preparation and handling.<br />

BD <strong>P100</strong> v1.1 <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong> enables greater<br />

recovery and preservation of plasma<br />

proteins by immediately mixing with<br />

proprietary novel on-board<br />

stabilizers, specifically formulated<br />

for human plasma.<br />

Preserve Valuable Plasma Proteins<br />

† Patented<br />

* BD <strong>P100</strong> is For Research Use Only – Not For Use in Diagnostics Procedures<br />

Highlights:<br />

• On-board stabilizers provide point-of-collection<br />

protection of plasma proteins<br />

• Enhances long-term preservation by stabilizing<br />

analytes during blood collection<br />

• Innovative mechanical separator † minimizes<br />

cellular contamination<br />

• Eliminates pre-mixing and transfer tubes<br />

• Sterile and ready to use<br />

BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> Tube Specifications:<br />

Total <strong>Blood</strong> Draw Volume: ~8.5 mL<br />

Plasma Yield: ~3.5 mL<br />

Anticoagulant (Spray Dried): K 2 EDTA<br />

Additive (Lyophilized): Proprietary Protein Stabilizers<br />

Storage Prior to Use: 2°C to 8°C


BD <strong>P100</strong> v1.1 Immediately Stabilizes Plasma Proteins<br />

BD <strong>P100</strong> v1.1 versus EDTA Plasma<br />

“Time 0” Gel Plot Summary of Eight Subjects (m/z 1000-2400)<br />

BD <strong>P100</strong> v1.1 versus EDTA Plasma<br />

“Time 0” Zoom Plot (m/z 1700-2050)<br />

Figure 1. Preservation of plasma proteins with BD <strong>P100</strong><br />

v1.1 <strong>Blood</strong> <strong>Collection</strong> <strong>Kit</strong>. <strong>Blood</strong> was drawn directly from<br />

eight subjects into <strong>P100</strong> and EDTA evacuated tubes. Plasma<br />

was processed within 10 minutes of blood collection by<br />

centrifugation at 2,500 x g for 15 minutes at room temperature.<br />

After blood collection, plasma was transferred into microtubes<br />

and prepared for mass spectrometry analysis or stored at –80ºC<br />

for future analysis. Peptides were isolated from the plasma<br />

samples and analyzed by MALDI-TOF MS. The mass spectra<br />

were collected in the mass range from 800 to 3200 m/z. Areas<br />

within the red rectangles demonstrate enhanced peptide<br />

stabilization in the <strong>P100</strong> samples versus EDTA plasma samples.<br />

A greater degree of proteolytic degradation is also more<br />

apparent in the EDTA plasma samples, even though samples<br />

were processed immediately upon blood collection (“Time 0”),<br />

as shown in the yellow rectangle areas.<br />

Figure 2. Enhanced stability of peptides observed in<br />

plasma samples collected with BD <strong>P100</strong> v1.1 <strong>Blood</strong><br />

<strong>Collection</strong> <strong>Kit</strong>. Regions with red arrows on this<br />

MALDI-TOF MS zoom plot (m/z 1700-2050) of two<br />

subjects demonstrates the ability of <strong>P100</strong> v1.1 to<br />

stabilize plasma peptides more effectively in the<br />

presence of protease inhibitors. These peaks are absent<br />

in the same regions on the EDTA zoom plots for both<br />

subjects. The immediate mixing of blood with the onboard<br />

protease inhibitors in BD <strong>P100</strong> tubes enhances<br />

the recovery of plasma proteins for proteomics studies.


Quantity Description<br />

12<br />

12<br />

12<br />

12<br />

<strong>Kit</strong> Contents<br />

BD <strong>P100</strong> <strong>Blood</strong> <strong>Collection</strong> Tubes<br />

Six (6) tubes/foil pouch, Two (2) foil pouches/kit<br />

BD Vacutainer ® One Use Tube Holders<br />

Single Use Only, Translucent<br />

BD Vacutainer ® Safety-Lok <strong>Blood</strong> <strong>Collection</strong> Set<br />

21 G Butterfly Needle, 12” (304.8 mm) Tubing<br />

Sterile, Use Once and Discard<br />

BD Alcohol Swab: Isopropyl Alcohol - 70%<br />

(Reference #366455 Only)<br />

1 Instructional Product Insert<br />

1 Research Use Only Certification Card<br />

North America<br />

#366455<br />

Reference Numbers<br />

Outside North America<br />

#366456


Ordering and Technical Information *<br />

Region Ordering Technical<br />

US<br />

888.237.2762 (phone orders)<br />

800.847.2220 (fax orders)<br />

800.631.0174<br />

(8:30 am – 5 pm) EST<br />

Canada 800.268.5430 ext. 5567 800.268.5430 ext. 5567<br />

All other<br />

areas<br />

Please visit www.bd.com<br />

to locate your local regional office<br />

To obtain more information on BD <strong>P100</strong> and other<br />

proteomics products, please visit our Web site<br />

www.bd.com/proteomics<br />

* BD <strong>P100</strong> is sold directly by BD Diagnostics – Preanalytical Systems<br />

Not available through distributors.


BD, BD Logo, and all other trademarks are property of Becton, Dickinson and Company. © 2007 BD<br />

03/07 VS7530-1<br />

1 Becton Drive<br />

Franklin Lakes, NJ 07417<br />

www.bd.com

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