Try High Affinity Tag protein Antibodies

WB IP ICCFCMAnti DYKDDDDKMonoclonal AntibodyClone: 2H8unocytochemistry Flow cytometorymWestern blottingImmunoprecipitation ImTryHigh AffinityTag proteinAntibodiesControl IgGDYKDDDDK mAb (2H8)MockFLAGhPGRhPGR-FLAGhPGR-Met-FLAGIP; Ab [μg/ml]M2 M5 2H8 M2 M5 2H8 M2 M5 2H8 M2 M5 2H80.1Sigma (M2)Sigma (M5)0.5IB; M511st Ab; 0.1 μg/mlICC in CHO cellby the DYKDDDDK(2H8) addition to N-terminus of PGRIP in CHO cell lysateby the DYKDDDDK(2H8) addition to N-terminus of PGRCount2H8 M2 M510 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4Log fluorescent intensityMockmBLT11 μg/ml FLAG mAbFLAG(2H8, M2 or M5)FLAG-mBLT11 μg/ml mlgG-ALEXA FLUOR ® 488CHO cellMouse BLT1ALEXA FLUOR ® is a registeredtrademark of Life Technologies.Relative intensityRelative intensity(FLAG-hS1P1 / Mock)(FLAG-hBLT1 / Mock)CHO-FLAG-hBLT12015107-fold500.001 0.01 0.1 1 101st Ab [μg / ml]CHO-FLAG-hS1P140302030-fold1000.001 0.01 0.1 1 101st Ab [μg / ml]Relative intensity(FLAG-mBLT1 / Mock)Relative intensity(FLAG-hCysLT1 / Mock)3T3-FLAG-mBLT16040203-fold00.001 0.01 0.1 1 101st Ab [μg / ml]CHO-FLAG-hCysLT130201025-fold00.001 0.01 0.1 1 101st Ab [μg / ml]Relativeintensity2H8M51st Ab [μg / ml](n=3, Mean + - S. D.)FCM in CHO cell (GPCR (mouse BLT1) stable expression)with DYKDDDDK(2H8) antibody ( 1 μg/ml : 2H8, M2, M5 ) andanti-Alexa488-mouse IgGFCM in CHO Cells (GPCR (mouse BLT1) stable expression)with DYKDDDDK(2H8) antibody( 0.3 μg/ml , 0.14 μg/ml, 0.04 μg/ml, 0.03 μg/ml: 2H8, M5 )and anti-Alexa488-mouse IgGProduct Name Catalog No. Host Clone Isotype SizeAnti DYKDDDDK Antibody KAL-KO602-S Mouse 2H8 IgG2a 5 μgKAL-KO602-M Mouse 2H8 IgG2a 200 μgKAL-KO602-L Mouse 2H8 IgG2a 1 mgBAM-60-031-EX Rabbit 100 μL

WBIPICCFCMunocytochemistry Flow cytometorymWestern blottingImmunoprecipitation ImHigh Affinity Tag protein AntibodiesAnti GFP polyclonal antibody (Rabbit)Cat# FRL-GFP-RB-AF2020-50-EX,FRL-GFP-RB-AF2020-20-EXAnti GFP polyclonal antibody (Goat)Cat# FRL-GFP-GO-AF1480-50-EXFRL-GFP-GO-AF1480-20-EXAnti GFP polyclonal antibody (Rabbit)Cat# BAM-60-011-EXSingle immunofluorescence for GFP in the somatosensory cortexour GFP antibodyother GFP antibody0.1 μg/ml 1.0 μg/ml 0.1 μg/ml 1.0 μg/mlOur goat anti-GFP (1 μg/ml)rabbit anti-GFP (1 μg/ml)from other companyGFP - +17580GAD-GFPlow gain58463025Wild-GFPlhigh gainWB : anti-GFPpolyclonal Ab(1 : 2,000)Anti GFP monoclonal antibody(Clone : 1A5)Cat# BAM-60-001-EXImmunofluorescent staing of the COS1cells with antibody 1A5Anti GST polyclonal antibody (Rabbit)Cat# BAM-60-021-EXIPinputGFP-INbox - + - + +175805846pre-immuneanti-GFPpolyclonal AdAnti Myc tag polyclonal antibody(Rabbit)Cat# BAM-60-041-EXIPinputmyc-NPM1 - + - + +175805846pre-immuneanti-mycpolyclonal Ad30253025GFP-expressed in COS1 cellsWB: anti-GFP monoclonal Ab- : Lysate of 293T cells transfected with an empty vector+ : Lysate of 293T cells transfected with the plasmidcarrying the GST-tagged INbox geneWB: anti-myc M9.5 monoclonal Ab- : Lysate of 293T cells transfected with an empty vector+ : Lysate of 293T cells transfected with the plasmid carryingthe Myc-tagged NPM1 geneProduct NameCatalog No. HostCloneIsotypeSizeAnti GFP(green fluorescent protein) AntibodyFRL-GFP-RB-AF2020-50-EX Rabbit 50 μgFRL-GFP-RB-AF2020-20-EX Rabbit 20 μgFRL-GFP-GO-AF1480-50-EX Goat 50 μgFRL-GFP-GO-AF1480-20-EX Goat 20 μgBAM-60-011-EX Rabbit 100 μLBAM-60-001-EX Rat 1A5 IgG1 100 μgAnti GST (Glutathione S transferase) Polyclonal antibodyBAM-60-021-EX Rabbit 100 μLAnti Myc tag Polyclonal Antibody BAM-60-041-EX Rabbit 100 μLFor research use only, Not for diagnostic use.www.cosmobio.com10067