New Brunswick BioFlo/CelliGen 115 brochure

Extra versatileNew Brunswick’s BioFlo ® /CelliGen ® 115 Autoclavable Fermentor andBioreactor handles a wide variety of cells to meet your every need

2Easy to use and versatile too!New Brunswick’s BioFlo ® /CelliGen ® 115New Brunswick Scientific’s BioFlo ® /CelliGen ® 115 is anexceptionally capable benchtop fermentor/bioreactor.Offered in 1.3 to 14 liter capacities, this system has beendesigned to provide the versatility to grow a wide varietyof cells, making it ideally suited for biotechnology andpharmaceuticals, biofuels, R&D, testing labs, academicinstitutes, and much more!> > All 115 systems are pre-programmed with bothfermentation and cell culture operating modes for totalflexibility. Switching between modes automatically adjustsgas flow and speed ranges> > Grow virtually any cell type: aerobic or anaerobic;microbes, yeast, insect, plant and mammalian cells> > The compact control station includes everything neededfor total process control: color touchscreen interface, threebuilt-in pumps, gas flow controllers for up to four gasses(air/O2/N2/CO2), foam/level sensors, pH/DO controllersand more> > Offered with water-jacketed or heat-blanketed vessels infour sizes: 1.3, 3, 7.5 and 14 Liters, total volume> > Pre-packaged fermentation and cell culturekits make ordering easy, or select from a widerange of options to meet your exact needs> > Single-use adapter kit available

4Easy to get going and start growingThe compact New Brunswick BioFlo ® /CelliGen ® 115 sets up in minutes.Adding extra vessels or a second/third utility station is plug-and-playsimple. No configuration needed.Control/Utility StationTouchscreen InterfaceWater-Jacketed VesselHeat-Blanketed VesselCompact design. CabinetStandard on controlShown with base heater,Shown with vessel stand,footprint measuresstations. Not providedexhaust gas condenserexhaust gas condenserjust 39.6 x 40.6 cmon utility stationsand magnetic drive motorand direct drive motor(15.6 x 16 in) W x DEasily AccessedConnectionsFor probes, motor,heater and spargeGas ControlUp to four Rotametersor a Thermal Mass FlowController. (TwoRotameters shown)SamplingAssemblyQuick ConnectsFor Water In/Out allowutilities to be attachedin secondsPeristaltic PumpsOn-OffOptional AdditionalThree fixed-speed (12 rpm)Easily accessed fromBottle Holderpumps can be linked directlythe right sideSaves bench spaceto acid, base, foam, level

5A wide range of options and accessories to meet yourspecific requirements4321Four impeller options provide flexibility to grow a widevariety of cell lines:1. Rushton impeller for standard fermentation applications2. Low-shear pitched blade3. Marine blade impellers for gentle mixing of shearsensitivecell lines (i.e. insect, plant and animal cultures)4. Spin filter — a cell-retention device used with amarine blade impeller — for perfusion processes usinganchorage-dependent or suspension culturesExceptional flexibiltyNumerous threaded ports provide a high degree of flexibilityfor positioning probes, sampling tube and exhaust gascondenser to suit your processPort SizeTotalVessel Size 6mm 12mm 19mm Ports1.3 L 1 9 0 103.0 L 6 7 0 137.5 L 7 8 1 1614.0 L 7 8 1 16A wide range of accessories allows easy customization

6Intelligent controlsOperation made simple> > Cell culture mode automatically mixes two, three or fourgasses for optimized cell growth. Two-gas mixing optionin fermentation mode enables mixing air and oxygen forhigh cell yields. Easily switch between modes throughtouchscreen controls.> > Highly customizable gas flow options allow you to designa system specific to your needs. Choose one, two, three orfour manual Rotameters of various flow rates. Or select adigital TMFC.> > Adjustable PID values for pH and DO are automaticallydefined by vessel size or can be fine tuned for the ultimate incontrol flexibility> > Free firmware updates are easily accessed from ourwebsite. No service technician or downtime> > Compatible with New Brunswick BioCommand ® softwarefor advanced control strategies and data loggingGauge screen: Change gas control modes and mix, customizedecimal displays, set deadbands or change PI settings all from loopgauge screens.Dissolved Oxygen (DO) Cascade: automates maintaining DOsetpoint through single- or multi-step cascade control.pH/DO Calibration screen: pH and DO probes are easily calibratedby selecting the probe and entering the zero and span.Pump screen: Control, calibrate and assign all three pumps fromone screen.

7Turn-key solutionsSimplify ordering; choose one of our pre-configured Basic or Advanced Kits. Or, design afully configured system with our many options. Easily add a second or third independentlyoperatedvessel; select a Vessel Kit and add a Control or Utility Station. Ask your rep aboutmore options.AdvancedFermentation KitAdvancedCell Culture KitBasicFermentation KitFermentor/Bioreactor Kit ContentsVessel Kit – BasicVessel Kit – AdvancedMaster Control Station (Touch Screen)Temperature ControlAgitation ControlpH/DO ControlFoam/Level Control3 Fixed-Speed PumpsManual Gas MixAutomatic Gas Mix (via 4 Solenoids)Manual Gas Flow (via Rotameters)Automatic Gas Flow (0-20 SLPM TMFC); available in configuredsystems only)Add-A-Vessel Kit ContentsHeatBlanketWaterJacketHeatBlanketWaterJacketHeatBlanketWaterJacketDish-Bottom Vessel with stainless steel headplateVessel StandAgitation Motor, 50 - 1200 rpm (ferm. direct drive)Agitation Motor, 25 - 200 rpm (cell cult. magnetic drive)Agitation Motor, 25 - 400 rpm (cell cult. direct drive)Heater BlanketJacket Water HeaterImmersion Cooling CoilThermowell & RTD ProbepH/DO Probe KitFoam/Level ProbeBaffle AssemblyRushton Impellers (two blades)Pitched Blade Impeller (one blade)Ring SpargerMicrospargeExhaust CondenserSampling AssemblyTri-Port AdapterSeptum KitLiquid Addition Tube and Headplate AdapterTwo Addition Bottles and TubingStandardOptional

Vessel specifications *Total Volume 1.3 Liters 3.0 Liters 7.5 Liters 14.0 LitersWorking volume 0.4 - 1.0 Liters 0.8 - 2.2 Liters 2.0 - 5.6 Liters 4.0 - 10.5 LitersDesignHeat-blanketed and water-jacketed • All vessels are borosilicate glass, autoclavable, with dished-bottomWeight 6.8 kg (15.0 lb) 9.3 kg (20.5 lb) 18 kg (39.5 lb) 19.5 kg (43.0 lb)BioFlo ® /Celligen ® 115 System specifications *Control Station & Utility StationDesignCompact control station with advanced integratedcontroller is capable of supporting up to 2 additional(optional) independent utility stations and vesselsDisplay21.3 cm (8.4 in) industrial color touchscreen display isstandard on the control stationNot included on optional utility stationsFunction Fermentation and cell culture monitoring and controlTemperatureRange1.3 - 7.5 L: 70ºC maximum temperature14 L: 65ºC maximum temperatureControlPID for heating and coolingHeat-blanketed Vessels: External heating blanket andimmersed stainless steel cooling coilWater-jacketed Vessels: Water jacket heater andcirculation loopSensor Platinum RTD probe (Pt 100)AgitationDriveMagnetic Drive or Direct DriveRangeDirect Drive: Ferm 50 - 1200 rpm;Cell Cult 25 - 400 rpm • Mag Drive: 25 - 200 rpmControlPID control; manual, automatic, or cascade settingsImpellers Rushton-style standard with fermentation system.Pitched blade standard with cell culture. Optional:Marine blade and/or Spin filterBafflesRemovable 316L stainless steel; fermentation onlypHRange2 - 14 pHControlPID, link to pumps or gasses, adjustable deadbandSensorpH probeDORange 0 - 200%ControlPID, cascade to agitation, gasses, gas flow if equippedwith TMFCSensorPolarographic DO probeAerationGas FlowOptionsGas MixingSpargerInlet FilterN 2GasExhaustFilterCondenser3 PumpsControl0 - 4 Rotameters: 0 -150 mLpm 250 - 2500 mLpm 1 -5 Lpm • 1 - 20 Lpm (and more)1 Thermal Mass Flow Controller (TMFC):0.04 - 20 SLPMOptions: Automatic 4-gas mixing & manual gasmixing. Both via 4-gas manifoldStandard: Ring SpargerOptional: Microsparger0.2 µm Absolute filterFor calibration of DO probe0.2 µm Absolute filterStainless steel counterflow, water-cooled in headplate60 Hz / 14.4 rpm50 Hz / 12 rpmUtility RequirementsWater10 PSIG maximum, 50 µm filtrationGasses10 PSIG maximumElectric Requirement100 - 230 V 50/60 Hz • Single Phase100 - 120V: 10 Amps; 200 - 240V: 6 AmpsDimensions (Height x Width x Depth)Control/UtilityStationWarrantyRegulatoryComlianceHeight: 67.6 cm (26.6 in)Width: 39.6 cm (15.6 in)Depth: 40.6 cm (16.0 in)One-year, parts and labor covering the entire systemexcept glassware. Probes: one-year manufacturer’swarranty. Factory-trained service technicians arelocated worldwide.*Specifications subject to change without notice. Not all options are included with all kits. Refer to page 7 for details.Your local distributor: www.eppendorf.com/worldwideEppendorf AG · Hamburg · Germany · Tel: +49 40 538 01-0Application Support E-mail: support@eppendorf.comEppendorf North America, Inc. · USA · Tel: +1 516 334 7500Toll free phone: +1 800 645 3050 menu option 2E-mail: techserv@eppendorf.comwww.eppendorf.comeppendorf ® is a registered trademark of Eppendorf AG, Hamburg, Germany. New Brunswick is a trademark of Eppendorf AG, Hamburg, Germany. BioFlo ® , CelliGen ® andBioCommand ® are registered trademarks of New Brunswick Scientific. All rights reserved, including graphics and images. Copyright© 2012 by Eppendorf AG. Order no. B-4027