Mapping, Plasmid, and Primer Design So, you have a DNA ...

bip.weizmann.ac.il

Mapping, Plasmid, and Primer Design So, you have a DNA ...

MAP• Displays the sequence and itscomplementary strand• Creates a restriction map of yoursequence• Can display a translation of thesequence in all frames, three forwardframes, the frame of your choice, orORFs (open reading frames)Restriction mapping:MAPall enzymes, type * or press returnno enzymes, press spacea specific enzyme, type in the enzymename, using the character i instead ofthe roman numeralexample: for HindIII type hindiiiMAPProgram options on the command linecan be used to limit the number of enzymes:-maxcuts=2-mincuts=2-oncFor example, I want any enzyme that cuts mysequence once or twice, so I type:%map -maxcuts=2 or%map sample.seq -maxcuts=2MAPProgram options on the command linecan be used to limit the number of enzymes:-maxcuts - allows me to choose the maximumnumber of cut sites-mincuts - allows me to choose the minimumnumber of cut sites-onc - gives me all the enzymes that cutare single cutters.

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