Mapping, Plasmid, and Primer Design So, you have a DNA ...

bip.weizmann.ac.il

Mapping, Plasmid, and Primer Design So, you have a DNA ...

Primer Design• Always make sure that you are inframe!• Double check the orientation ofthe sequence before you submitit for synthesis!Primer DesignAlways sequence PCR products!!!!(preferably after subcloning, unlessyou are just checking for presenceof product)1 60233 GATAAGCTTG ATATCGAATT GCCA.GTTGA AGCCATCATT ACCATTCACA TCCCTCTTGT240 GATAAGCTTG ATATCGAATT GCCATGTTGA AGCCATCATT ACCATTCACA TCCCTCTTAT239 GATAAGCTTG ATATCGAATT GCCATGTTGA AGCCATCATT ACCATTCACA TCCCTCTTATgamma G AAGAGCAAGC GCCATGTTGA AGCCATCATT ACCATTCACA TCCCTCTTAT61 120233 TCCTGCAGCT GCCCCTGCTG GGAGTGGGGC TGAACACGAC AATTCTGACG CCCAATGGGA240 TCCTGCAGCT GCCCCTGCTG GGAGTGGGGC TGAACACGAC AATTCTGACG CCCAATGGGA239 TCCTGCAGCT GCCCCTGCTG GGAGTGGGGC TGAACACGAC AATTCTGACG CCCAATGGGAgamma TCCTGCAGCT GCCCCTGCTG GGAGTGGGGC TGAACACGAC AATTCTGACG CCCAATGGGA121 180233 ATGAAGACAC CACAGCTG.. .......... .......... ......ATTT CTTCCTGACC240 ATGAAGACAC CACAGCTGGT GGGAAATCTG GGACTGGAGG GGGCTGATTT CTTCCTGACC239 ATGAAGACAC CACAGCTG.. .......... .......... ......ATTT CTTCCTGACCgamma ATGAAGACAC CACAGCTG.. .......... .......... ......ATTT CTTCCTGACC181 210233 ACTATGCCCA CTGACTCCCT CAGTGTTTCC240 ACTATGCCCA CTGACTCCCT CAGTGTTTCC239 ACTATGCCCA CTGACTCCCT CAGTGTTTCCgamma ACTATGCCCA CTGACTCCCT CAGCGTTTCC

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