Muna Naash, Ph.D. - OU Medicine

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Muna Naash, Ph.D. - OU Medicine

CURRICULUM VITAE

Muna I. Naash

Professor of Cell Biology and Neuroscience

Director of the Cell Biology Graduate Program

I. Personal

Address University of Oklahoma Health Sciences Center

Department of Cell Biology, BMSB 781

940 Stanton L. Young Blvd.

Oklahoma City, OK 73104

Citizenship U.S.A

Phone: (405) 271-2388

Fax: (405) 271-3548

E-mail: muna-naash@ouhsc.edu

Web Site: http://w3.ouhsc.edu/cell_biology/Naash_M.asp

II. EDUCATION

1990-1992 Postdoctoral Fellow, Department of Ophthalmology, Cullen Eye Institute, Baylor College

of Medicine, Houston, TX., Supervisor Dr. Wolfgang Baehr

1990 Ph.D. Biochemistry, Department of Biochemistry, Baylor College of Medicine, Houston

TX., Supervisor Dr. Robert E. Anderson

1982-1983 Pre-doctoral Fellow, Department of Biochemistry, Baylor College of Medicine, Houston

TX.

III. SCIENTIFIC EMPLOYMENT/ ACADEMIC APPOINTMENTS

2006-present Professor, Department of Cell Biology and Oklahoma Center for Neuroscience,

University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK.

2004-present Director of Cell Biology Graduate Program, Department of Cell Biology, OUHSC, OK.

2004-present Member, OU Cancer Center, OUHSC, Oklahoma City, OK.

2000-present Associate Professor, Department of Cell Biology and Oklahoma Center for

Neuroscience, OUHSC, Oklahoma City, OK.

1998–2000 Associate Professor, Department of Ophthalmology and Visual Science, and

Department of Molecular Genetics, University of Illinois at Chicago (UIC), Chicago, IL.

1993-1998 Assistant Professor, Department of Molecular Genetics, UIC, Chicago, IL.

1992-1998 Assistant Professor, Department of Ophthalmology and Visual Science, UIC, Chicago,

IL.

1990-1992 Postdoctoral Fellow, Cullen Eye Institute, Baylor College of Medicine (BCM), Houston,

TX.

1986-1990 Predoctoral Fellow, Department of Biochemistry, BCM, Houston, TX.

1983-1986 Research Associate, Department of Ophthalmology, BCM, Houston, TX.

1982-1983 Predoctoral Fellow, Department of Biochemistry, BCM, Houston, TX.

IV. RESEARCH AND SCHOLARSHIP

Research Grants and Contracts

CURRENT

Title: Compacted DNA Nanoparticles for Ocular Therapy

Agency: NIH/National Eye Institute R01 (EY018656) PI: Naash

Duration: 2008-2012

Title: Gene Therapy for Ocular Diseases Using Nanotechnology PI: Naash

Agency: Oklahoma Center for the Advancement of Science and Technology (OCAST)

Duration: 2007-2010

Title: Delivery and targeting of ocular therapeutics using nanotechnology

Agency: Southwest Center of the Foundation Fighting Blindness (SW-FFB) PI: Naash

CURRICULUM VITA FOR MUNA I. NAASH, PHD 1


Duration: 2007-2012

Title: Mechanism of photoreceptor cell degeneration in animal model of human retinal

diseases

Agency: NIH/National Eye Institute R01 (EY010609) PI: Naash

Duration: 2005-2009

Title: Evaluation the efficacy of Acucela drug on ABCR null mice.

Agency: Acucela Inc. PI: Naash

Duration: 2006-2008

Title: Sustained, Non-viral Ocular Therapy Using Nanoparticles

Agency: NIH/National Eye Institute R03 (EY016201) PI: Naash

Duration: 2005-2008

Title: Stem cell therapy in animal models of retinal degeneration PI: Naash

Agency: Johnson & Johnson Pharmaceutical

Duration: 2005-2008

Title: Oklahoma IDeA Network of Biomedical Research Excellence PI: Waxman

Agency: NIH-INBRE, P20 RR016478

Duration: 2004-2009

Role: Mentor

Title: Core Grant for Vision Research PI: Anderson

Agency: NIH/NEI - P30 EY12190-09

Duration: 2004-2009

Role: Member

PAST/COMPLETED

Oklahoma Center for the Advancement of Science and Technology (OCAST), PI: Naash (Gene Therapy

for Ocular Diseases Using Nanotechnology), 2004-2007.

Foundation Fighting Blindness, Inc., PI: Naash, (Delivery and targeting of ocular therapeutics using

nanotechnology), 2004-2007.

Macular Society, PI: Naash, (Non-viral gene therapy to rescue a mouse model of macular dystrophy),

2005-2006.

NIH/NEI, R01 (EY10609), PI: Naash (supplement for transgenic mice rederivation), 2004-2006

INSPIRE Pharmaceuticals, Inc., PI: Naash, (Microarray studies to isolate modulating genes by the P2Y2

Receptor Agonists in Mouse Model of retinal detachment).

NIH/NEI (Mentoring Vision Research in Oklahoma), Mentor, 2002-2007.

NIH/NEI, R01 (EY10609), PI: Naash (Mechanisms of photoreceptor cell degeneration in animal models

for human retinal diseases),1999-2005, $1,830,440.

Oklahoma Center for the Advancement of Science and Technology (OCAST), PI: Naash (Ribozyme

Therapy for Stationary Night Blindness), 2001-2004.

INSPIRE Pharmaceuticals, Inc., PI: Naash, (Therapeutic Application of P2Y2 Receptor Agonists in

Transgenic Mouse Models of Retinal Degeneration).

The Foundation Fighting Blindness, PI: Naash, (Efficiency and specificity of ribozyme therapy), 2000-

2003.

Research to Prevent Blindness, PI: Naash, (Rescue of visual loss in animal models for retinal diseases),

2000.

The Foundation Fighting Blindness, PI: Naash, (Ribozyme therapy for rhodopsin mutations that cause

autosomal dominant retinitis pigmentosa) 1999-2002.

March of Dime, Co-PI: Naash, (Generation of transgenic mice expressing P23H mutation in the human

opsin gene), 1999-2000.

The Foundation Fighting Blindness, PI: Naash, (UIC Center Grant- Studies on the mechanism of

photoreceptor cell death), 1997-2000.

Macular Society, PI: Naash, (Animal model for juvenile macular degeneration due to a mutation in

peripherin/rds gene), 1998-1999.

The Foundation Fighting Blindness, PI: Naash, (Ribozyme therapy for autosomal dominant retinitis

pigmentosa), 1996-1999.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 2


NIH/NEI, R01 (EY10609), PI: Naash, (Photoreceptor cell degeneration in animal models of human

retinal diseases), 1995-1998.

NIH/NEI, Shannon Award for the R01 (EY10609), PI: Naash, (Photoreceptor cell degeneration in animal

models of human retinal diseases), 1994-1995.

Young Investigator Award from the Foundation Fighting Blindness, PI: Naash, (Mechanism of

photoreceptor cell degeneration in a transgenic mouse line that mimic ADRP), 1993-

1996.

Fight for Sight, PI: Naash, (Expression of P216L mutant peripherin/rds gene in transgenic mice as a

model for one form of human ADRP).

Midwest Eye Bank and Transplantation Center (Seven awards), PI: Naash, (to support our research on

the treatment of retinitis pigmentosa), 1993-2000..

Campus Research Board at University of Illinois at Chicago (Four awards), PI: Naash, (to support our

research on the biochemical role of peripherin/rds in the retina), 1992-2000.

Illinois Society for the Prevention of Blindness (Six awards), PI: Naash, (to support our research on the

generation of animal models for cone degeneration), 1993-1999.

Illinois Eye Fund at UIC Eye Center (Five awards), PI: Naash, (to support our ongoing research on the

treatment of retinitis pigmentosa models), 1993-1996.

Knights Templar Eye Foundation (Ten awards), PI: Naash, (to support our ongoing research on the

generation of animal models for cone dystrophy), 1992-2006, Two awards for Dr.

Naash and 8 for her postdoctoral fellows.

NRSA-NIH/NEI, PI: Naash, (Mouse models for retinal degenerations), 1990-1992, Postdoctoral

fellowship.

NIH/NEI, Co-PI: Naash, (Isolation and characterization of ubiquitin system from the rat retina), 1988-

1989.

V. PUBLICATIONS

Published in Refereed Journals

1. Conley S & Naash MI. (2008) Ocular gene therapy: assessment and future directions. Current Opinion in

Molecular Therapeutics. In Press

2. Chakraborty D, Ding X-Q, Fliesler SJ & Naash MI. (2008) Outer segment oligomerization of Rds: evidence

from mouse models and subcellular fractionation. Biochemistry. 47:1144-1156 (http://pubs.acs.org/cgibin/article.cgi/bichaw/2008/47/i04/pdf/bi701807c.pdf).

3. Elliott MH, Nash ZA, Takemori N, Fliesler SJ, McClellan ME & Naash MI. (2008) Differential distribution of

proteins and lipids in detergent-resistant and detergent-soluble domains in rod outer segment plasma

membranes and disks. J Neurochem. 104:336-352 (http://www.blackwellsynergy.com/doi/pdf/10.1111/j.1471-4159.2007.04971.x).

4. Farjo R, Peterson WM & Naash MI. (2008) expression profiling following retinal detachment and

reattachment: a role for aquaporin-0. Invest Ophthalmol. Vis. Sci. 49:511-521

(http://www.iovs.org/cgi/reprint/49/2/511).

5. Nour M, Fliesler SJ & Naash MI (2008) Genetic supplementation of Rds alleviates a loss-of-function

phenotype in C214S model of retinitis pigmentosa. Adv Exp Med Biol. 613:129-138.

6. Chakraborty D, Whalen P, Lewin A, & Naash MI (2008) Ribozyme-Mediated Treatment of P23H rhodopsin

model of ADRP. Adv Exp Med Biol. 613:97-106.

7. Cai X, Conley S, and Naash MI (2008) Nanoparticle applications in ocular gene therapy. Vision

Res.,48:319-324.

8. Conley S, Nour N, Fliesler SJ, & Naash MI. (2007) Late onset cone photoreceptor degeneration induced by

the R172W mutation in Rds and partial rescue by gene supplementation. Invest Ophthalmol. Vis. Sci. 12-

5397-407 (http://www.iovs.org/cgi/reprint/48/12/5397).

9. Farjo R, Fliesler SJ and Naash, M.I. (2007) The Effect of Rds Abundance on Cone Outer Segment

Morphogenesis, Photoreceptor Gene Expression and Outer Limiting Membrane Integrity. J. Comp. Neurol.,

504:619-630 (http://www3.interscience.wiley.com/cgi-bin/fulltext/115806201/PDFSTART).

10. Canola K, Angénieux B, Tekaya M, Quiambao A, Naash MI, Munier FL, Schorderet DF, Arsenijevic Y.

(2007) Retinal stem cells transplanted into models of late stages of retinitis pigmentosa preferentially adopt

a glial and a retinal ganglion cells fate. Invest Ophthalmol. Vis. Sci. 48:446-454

(http://www.iovs.org/cgi/reprint/48/1/446).

11. Farjo, R. and Naash, MI (2006) The role of rds in outer segment morphogenesis and human retinal disease.

Ophthalmic Genetics 27:117-22.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 3


12. Farjo R, Skaggs J, Quiambao AB, Cooper MJ and Naash, M.I. (2006) Efficient non-viral ocular gene transfer

with compacted DNA nanoparticles. PloS ONE. 20;1:e38

(http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000038).

13. Farjo R, Skaggs J, Nash Z, Quiambao AB, Nagel BA, Fliesler SJ and Naash, M.I. (2006) Retention of

function without normal disc morphogenesis occurs in cone but not in rods photoreceptors. J. Cell Biol.

173:59-68 (http://www.jcb.org/cgi/reprint/173/1/59).

14. Samardzija M, Wenzel A, Naash, M.I., Remé CE, Grimm C. (2006) Rpe65 as a modifier gene for inherited

retinal degeneration. Submitted to Molecular Vision. European Journal of Neuroscience, 23:1028-1034

(http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1460-9568.2006.04639.x).

15. Ding X-D and Naash MI (2006) Transgenic animal studies of human retinal disease caused by mutations in

peripherin/rds. Adv Exp Med Biol.572:141-146.

16. Nash Z and Naash MI (2006) Light/dark translocation of alpha-transducin in mouse photoreceptor cells

expressing G90D mutant opsin. Adv Exp Med Biol. 572:125-139.

17. Stricker H, Ding X-Q, Quiambao AB, Fliesler SJ, and Naash, M.I. (2005) The Cys 214 →Ser mutation in

peripherin/rds causes a loss-of-function phenotype in transgenic mice. Biochemical J. 388:605-613

(http://www.biochemj.org/bj/388/0605/3880605.pdf).

18. Ding X-Q, Stricker H and Naash, M.I. (2005) Role of the second intradiscal loop of peripherin/rds in homo

and hetero associations. Biochemistry. 44:4897-904 (http://pubs.acs.org/cgibin/article.cgi/bichaw/2005/44/i12/pdf/bi048414i.pdf).

19. Nour, M, Al-Ubaidi, MR and Naash, M.I. (2004) Absence of functional and structural abnormalities

associated with expression of EGFP in the retina. Invest Ophthalmol. Vis. Sci. 45:15-22

(http://www.iovs.org/cgi/reprint/45/1/15).

20. Tan E., Ding, X-Q., Agarwal, N., Naash, M.I., Al-Ubaidi, M.R. (2004) Expression of cone photoreceptor

specific antigens in a cell line derived from retinal tumors in transgenic mice. Invest Ophthalmol. Vis. Sci.

45:764-768 (http://www.iovs.org/cgi/reprint/45/3/764).

21. Grimm C, Wenzel A, Stanescu D, Samardzija M, Hotop S, Groszer M, Naash, M.I., Gassman M, Remé C.

(2004) Constitutive overexpression of human erythropoietin in the mouse retina: Protection against induced

but not against inherited retinal degeneration. J. Neurosc. 24:5651-5658

(http://www.jneurosci.org/cgi/reprint/24/25/5651).

22. Nour, M, Ding, X-Q, Stricker, H, Fliesler, SJ and Naash, M. I. (2004) Modulating expression of peripherin/rds

in transgenic mice: critical levels and the effect of over-expression. Invest Ophthalmol. Vis. Sci. 45:2514-21

(http://www.iovs.org/cgi/reprint/45/8/2514).

23. Naash, M.I., Wu T-H, Chakraborty D, Fliesler SJ, Ding X-Q, Nour M, Peachey NS, Lem J., Qtaishat N, Al-

Ubaidi MR, and Ripps H (2004) Retinal Abnormalities Associated with the G90D Mutation in Opsin. J.

Comparative Neurology. J Comp Neurol. 478:149-63 (http://www3.interscience.wiley.com/cgibin/fulltext/109607150/PDFSTART).

24. Ding X-Q, Nour M, Ritter LM, Goldberg AF, Fliesler SJ and Naash, M.I. (2004) The R172W Mutation in

Peripherin/rds Causes Cone-Rod Dystrophy in Transgenic Mice. Hum Mol Genet. 13:2075-87

(http://hmg.oxfordjournals.org/cgi/reprint/13/18/2075).

25. Li C., Ding X-Q, O’Brien J, Al-Ubaidi M R and Naash, M.I. (2003) The skate peripherin/rds gene: structural

and evolutionary relationship to other homologues. Invest Ophthalmol. Vis. Sci. 44:2433-2441

(http://www.iovs.org/cgi/reprint/44/6/2433).

26. Nour, M, Peterson, WM, Al-Ubaidi, MR and Naash, M. I. (2003) P2Y2 receptor agonist INS37217 enhances

functional recovery following detachment caused by subretinal injection in normal and RDS mice. Invest

Ophthalmol. Vis. Sci. 44:4505-14 (http://www.iovs.org/cgi/reprint/44/10/4505).

27. Naash, M.I., Ding, X-Q, Li C, O’Brien, J., and Al-Ubaidi, M.R (2003) Peripherin/rds works alone in the skate

retina. In: Retinal Degenerations: Mechanisms and Experimental Therapy. Adv Exp Med Biol. 533:377-83.

28. Nour, M and Naash, M.I. (2003) Mouse models of human retinal diseases caused by expression of mutant

rhodopsin. In: Retinal Degenerations: Mechanisms and Experimental Therapy. Adv Exp Med Biol. 533:173-

9.

29. Naash, M.I. (2002) Therapeutic interventions to rescue vision in animal models of human retinal diseases.

Emirates Medical J. 20:113-116.

30. Quiambao, AB, Tan, E, Chang, S, Komori, N, Naash, M.I., Peachey, NS, Matsumoto, H, Ucker, DS, Al-

Ubaidi, MR (2001) Transgenic Bcl-2 expressed in photoreceptor cells confers both death-sparing and deathinducing

effects. Exp. Eye Res. 73:711-721

(http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WFD-4582KW2-2K-

1&_cdi=6792&_user=2967949&_orig=search&_coverDate=11%2F30%2F2001&_sk=999269994&

view=c&wchp=dGLbVlz-zSkWb&md5=fee99f53427b95fb3aa8a7f89795ee6e&ie=/sdarticle.pdf).

CURRICULUM VITA FOR MUNA I. NAASH, PHD 4


31. Tan, E., Wang, Q., Quiambao, A. B., Xu, X., Qtaishat, N. M., Peachey, N.S., Lem, J., Fliesler, S. J.,

Pepperberg, D. R., Naash, M.I., Al-Ubaidi, M. R. (2001) Photoreceptor Degeneration Due to Over-

Expression of Opsin. Invest Ophthalmol. Vis. Sci. 42:589-600 (http://www.iovs.org/cgi/reprint/42/3/589).

32. Li, C, Cheng, M, Yang, H, Peachey, N.S and Naash, M.I. (2001) Age-related changes in the mouse outer

retina, Optometry and Vision Science. 78:425-430

(http://ovidsp.uk.ovid.com/spa/ovidweb.cgi?WebLinkFrameset=1&S=APHAPDLFOHHFDNFHFNHLJEJHNH

PPAA00&returnUrl=http%3a%2f%2fovidsp.uk.ovid.com%2fspa%2fovidweb.cgi%3f%26Full%2bText%3dL%

257cS.sh.15.16%257c0%257c00006324-200106000-

00015%26S%3dAPHAPDLFOHHFDNFHFNHLJEJHNHPPAA00&directlink=http%3a%2f%2fgraphics.uk.ovi

d.com%2fovftpdfs%2fPDHFFNJHJEFHOH00%2ffs047%2fovft%2flive%2fgv024%2f00006324%2f00006324

-200106000-00015.pdf&filename=Age-Related+Changes+in+the+Mouse+Outer+Retina).

33. Anderson, RE, Sieving, PA, Bush, RA, Maude, MB, Wu, T-H, Naash, M.I. (2001) DHA levels in rod outer

segments of transgenic mice expressing G90D rhodopsin mutations. New Insights into Retinal Degenerative

Diseases ed. M.M. ed. R.E. Anderson, M.M. LaVail, and J.G. Hollyfield. Alan R. Liss (New York). P235-245.

34. Li, C, O’Brien, J., Al-Ubaidi, M.R. and Naash, M.I. (2001) Organization of the chicken and xenopus

peripherin/rds gene. New Insights into Retinal Degenerative Diseases ed. M.M. ed. R.E. Anderson, M.M.

LaVail, and J.G. Hollyfield. Alan R. Liss (New York). P269-277.

35. Cheng, T. and Naash, M.I. and Al-Ubaidi, M.R. (2001) Application of polymerase chain reaction in deletion

and inversion of internal DNA fragments. New Insights into Retinal Degenerative Diseases ed. M.M. ed.

R.E. Anderson, M.M. LaVail, and J.G. Hollyfield. Alan R. Liss (New York). P247-253.

36. Penn, J.S., Li, S., and Naash, M.I. (2000) Ambient hypoxia reverses retinal vascular attenuation in a

transgenic mouse model of autosomal dominant retinitis pigmentosa. Invest Ophthalmol. Vis. Sci. 41:4007-

4013 (http://www.iovs.org/cgi/reprint/41/12/4007).

37. Ren, J-C, Stubbs, E,. Matthes, MT, Yasumura, D, Naash, M.I., LaVail, M, and. Peachey, NP (2000) Retinal

degeneration in the nervous mutant mouse. IV. Inner retinal changes. Ex. Eye Res.72:243-252

(http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WFD-458W960-5-

1&_cdi=6792&_user=2967949&_orig=search&_coverDate=03%2F31%2F2001&_sk=999279996&view=c&w

chp=dGLzVzz-zSkzk&md5=076203a38d3aa5f112372a5ecabdcb9f&ie=/sdarticle.pdf).

38. Qtaishat, N.M., Li, S., Naash, M.I. and Pepperberg, D.R. (1999) Retinoid kinetics in the VPP mouse, a

model of autosomal dominant retinitis pigmentosa. Invest Ophthalmol. Vis. Sci. 40:1040-1049

(http://www.iovs.org/cgi/reprint/40/6/1040).

39. Cheng, I. and Naash, M.I. (1999) The peripherin/rds gene: Structural and functional analyses. Degenerative

Retinal Diseases and Experimental Therapy, ed. M.M. LaVail, R.E. Anderson and J.G. Hollyfield. Alan R.

Liss (New York). Pp 235-249.

40. Wu, T-H., Ting, T.D., Okajims, T-I.L., Pepperberg, D.R., Ho, Y-K, Ripps, H. and Naash, M.I. (1998) Opsin

localization and Rhodopsin Photochemistry in a Transgenic Mouse model of retinitis pigmentosa.

Neuroscience. 87:709-717 (http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T0F-

3VGC6PT-17-

C&_cdi=4861&_user=2967949&_orig=search&_coverDate=08%2F04%2F1998&_sk=999129996&view=c&

wchp=dGLbVzW-zSkWb&md5=3815fd5f3b3da89da43b4ab3db62dbd4&ie=/sdarticle.pdf).

41. Wang, M., Lam, T., Tso, M. and Naash, M. I. (1997) Expression of mutant opsin gene increases the

susceptibility of the retina to light damage. Vis. Neurosc. 14:55-62.

42. Cheng, T, Al-Ubaidi, M.R. and Naash, M.I. (1997) Structural and Developmental analysis of the mouse

peripherin/rds gene. Somat. Cell Mol. Genet. 23:165-183.

43. Naash, M.I., Al-Ubaidi, M.R. and Anderson, R. E. (1997) Light exposure induces ubiquitin conjugation and

degradation activities in the rat retina. Invest. Ophthalmol. Vis. Sci. 38:2344-2354

(http://www.iovs.org/cgi/reprint/38/11/2344).

44. Liu, X Wu, T, Stowe, S., Matsushita, A., Arikawa, K., Naash, M.I. and Williams, D. (1997) Defective

phototransductive disk membrane morphogenesis in transgenic mice expressing rhodopsin with a mutated

N-terminal domain. J. Cell Science. 110:2589-2597 (http://jcs.biologists.org/cgi/reprint/110/20/2589).

45. Cheng, T, Peachey, N.S., Li, S, Goto, Y, Cao, Y and Naash, M.I. (1997) The effect of peripherin/rds

haploinsufficiency on rod and cone photoreceptors. J. Neurosc. 17:8118-8128

(http://www.jneurosci.org/cgi/reprint/17/21/8118).

46. Peachey, N.S, Wang M., and Naash, M.I. (1997) The VPP mouse: a transgenic model of autosomal

dominant retinitis pigmentosa. Degenerative Retinal Diseases, ed. M.M. LaVail, R.E. Anderson and J.G.

Hollyfield. Alan R. Liss (New York). pp 89-97.

47. Goto, Y., Peachey, N., Ziroli, N., Seiple, W., Gryczan, C. Pepperberg, D. and Naash, M.I. (1996) Rod

phototransduction in a transgenic mouse model of autosomal dominant retinitis pigmentosa. J. Optical Soc.

Amer A 13:577-585.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 5


48. Naash, M.I., Peachey, N.S., Li, Z-Yi, Gryczan, C.C., Goto, Y., Blanks, J., Milam, A.H. and Ripps, H. (1996)

Light-Induced Acceleration of Photoreceptor Degeneration in Transgenic Mice Expressing Mutant Opsin.

Invest. Ophthalmol. Vis. Sci. 37:775-782 (http://www.iovs.org/cgi/reprint/37/5/775).

49. Naash, M.I., Ripps, H., Shihong Li, Goto, Y. and Peachey, N.S. (1996) Polygenic disease and retinitis

pigmentosa: albinism exacerbates photoreceptor degeneration induced by the expression of a mutant opsin

in transgenic mice. J. Neurosc. 16:7853-7858 (http://www.jneurosci.org/cgi/reprint/16/24/7853).

50. Goto, Y., Peachey, N.S., Ripps, H. and Naash, M.I. (1995) Functional abnormalities in transgenic mice

expressing a mutant rhodopsin gene. Invest. Ophthalmol. Vis. Sci. 36:62-71

(http://www.iovs.org/cgi/reprint/36/1/62).

51. Peachey, N.S. Goto, Y. Al-Ubaidi, M.R. and Naash, M.I. (1994) Rod and cone dysfunction in transgenic

mice, in: "Vision Science and its Applications, Technical Digest Series, Vol. 2", Anonymous Optical Society

of America, Washington, D.C., pp. 362-365 (1994).

52. Naash, M.I., Hollyfield, J.G., Al-Ubaidi, M.R. and Baehr, W. (1993) Simulation of human autosomal

dominant retinitis pigmentosa in transgenic mice expressing a mutated murine opsin gene. Proc. Nat. Acad.

Sci., USA 90:5499-5503.

53. Peachey, N.S., Goto, Y., Al-Ubaidi, M.R. and Naash, M.I. (1993) Properties of the mouse cone-mediated

electroretinogram during light adaptation. Neurosc. Lett. 162:9-11

54. Naash, M.I., Al-Ubaidi, M.R., Hollyfield, J. G. and Baehr, W. (1993) Simulation of ADRP by introducing

mouse opsin mutations into transgenic mice. In Retinal Degeneration: Experimental and Clinical Studies,

ed. M.M. LaVail, R.E. Anderson and J.G. Hollyfield. Alan R. Liss (New York), pp201-210.

55. Penn, J.S., Thum, L. A. and Naash, M.I. (1992) Oxygen-induced retinopathy in the rat: Vitamin C and E as

potential Therapies. Invest. Ophthalmol. Vis. Sci. 33:1836-1845 (http://www.iovs.org/cgi/reprint/33/6/1836).

56. Naash, M.I., Izabicka, E. and Anderson, R.E. (1991) The retina has an active and stable ubiquitin-protein

conjugating system. J. Neurosci. Res. 30:433-441.

57. Naash, M.I. and Anderson, R.E. (1989) Glutathione-dependent enzymes in intact rod outer segments of

rats. Exp. Eye Res. 48:309-318.

58. Penn, J.S., Thum, L.A. and Naash, M.I. (1989) Photoreceptor physiology in the rat is governed by the light

environment. Exp. Eye Res. 49: 205-215.

59. Naash, M.I., LaVail, M.M. and Anderson, R.E. (1989) Factors affecting the susceptibility of the retina to light

damage. In Retinal Degeneration: Experimental and Clinical Studies, ed. M.M. LaVail, R.E. Anderson and

J.G. Hollyfield. Alan R. Liss (New York), pp 513-522.

60. Nielsen, J.C., Naash, M.I. and Anderson, R.E. (1988) The regional distribution of vitamins E and C in

premature human retina. Invest. Ophthalmol. Vis. Sci. 29:22-26 (http://www.iovs.org/cgi/reprint/29/1/22).

61. Naash, M.I. and Anderson, R.E. (1988) Regional distribution of glutathione peroxidase and glutathione-stransferase

in adult and premature human retinas. Invest. Ophthalmol. Vis. Sci. 29:149-152

(http://www.iovs.org/cgi/reprint/29/1/149).

62. Penn, J.S., Naash, M.I. and Anderson, R.E. (1987) Effect of light history on retinal antioxidants and light

damage. Exp. Eye Res. 44:779-788.

63. Anderson, R.E., Wiegand, R.D., Rapp, L.M., Maude, M.B., Naash, M.I. and Penn, J.S. (1987) Studies on

biochemical mechanisms of retinal degeneration. In Cell and Developmental Biology of the Eye. 6, The

Microenvironment and Vision, ed. J.B. Sheffield and S.R. Hilfer. Springer-Verlag, pp. 159-168.

64. Rapp, L.M., Naash, M.I., Wiegand, R.D., Joel, C.D., Nielsen, J.C. and Anderson, R.E. (1985) Morphological

and biochemical comparisons between retinal regions having differing susceptibility to photoreceptor

degeneration. In Retinal Degeneration: Experimental and Clinical Studies, ed. M.M. LaVail, J.G. Hollyfield,

and R.E. Anderson. Alan R. Liss, Inc. (New York), pp. 421-437.

65. Naash, M.I. and Anderson, R.E. (1984) Characterization of glutathione peroxidase in frog retina. Curr. Eye

Res. 3:1299-1304.

66. Al-Mudhaffar, S. A. and Naash, M.I. (1980) Kinetics of alkaline phosphatase in serum of liver diseased,

anemic and normal individuals. Biochem. Exp. Bio. 16:141-155.

Manuscripts Submitted or in Preparation for Submission

67. Cai X, Conley SM, Nash Z, Fliesler SJ, Cooper MJ, Naash MI. Self compacted DNA nanoparticles directed

to rods can efficiently rescue retinitis pigmentosa model. Gene Therapy

68. Conley SM, Sherry D, Fliesler SJ, Naash MI. Functional knockout of Aquaporin 0 selectively disrupts

synaptic connections between cones and ON-cone bipolar cells. PloS Cell Biology.

69. Cai X, Nash Z, Conley SM, Fliesler SJ, Cooper MJ, Naash MI. Self compacted DNA nanoparticles restore

vision in a mouse model of retinitis pigmentosa. Nature Medicine.

70. Conley S and Naash, MI. A transgenic mouse model for autosomal dominant retinitis pigmentosa caused by

a three base pair deletion in codon 255/256 of the opsin gene.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 6


71. Conley S, Nash Z, Fliesler SJ, & Naash MI. Evaluation of a macular dystrophy genetic mutation in a conedominated

murine retina.

72. Chakraborty, D, Ding, X-Q, Quiambao, A, Fliesler, SJ and Naash, M.I. Effects of C150S mutation in

peripherin/rds on cones dominant retina. In preparation for submission.

73. Cai X, Cheng, T and Naash, MI. The mouse Rds gene: Cloning and characterization of the 5’-flanking and

promoter region.

74. Chakraborty D, Ding X-Q, Fliesler SJ & Naash MI. Differential role of intermolecular disulfide linkage

necessary for Rds subunit assembly in rods versus cones.

75. Stricker H, Conley S, & Naash. Biochemical mechanisms behind rod- and cone-specific diseases initiated

by mutations at position 244 in Rds.

Abstracts Presented at Scientific Meetings Since 2004

1. Conley SM, Sherry D.M., Arbogast K.L., Nagel B.A., Shackelford J.R., Fliesler S.J., Naash MI. (2008)

Aquaporin 0 is Crucial for Proper Synapse Formation between Cones and On Cone Bipolar Cells. Invest.

Opthalmol. Vis. Sci. ARVO.

2. X. Cai, S.M. Conley, M.J. Cooper, S.J. Fliesler, M.I. Naash (2008) Targeting the RPE: A novel application

for compacted-DNA nanoparticles. Invest. Opthalmol. Vis. Sci. ARVO

3. Cai X, Cooper MJ and Naash MI (2008) Assessment of Compacted-DNA nanoparticle-mediated gene

therapy in a model of Leber congenital amaurosis. 11 th Annual Meeting of ASGT, May 27-June 1, Boston,

MA

4. Conley S, Cooper M, Naash MI (2007) Expression and Distribution of Compacted-DNA Nanoparticles in the

Developing Retina. 10 th Annual Meeting of ASGT, May 30-June 3, 2007. Seattle, WA.

5. Cai X, Fliesler SJ, Cooper MJ, Naash MI (2007) Functional rescue of the Rds haploinsufficency phenotype

with non-viral compacted DNA nanoparticles. 10 th Annual Meeting of ASGT, May 30-June 3, 2007. Seattle,

WA.

6. Agbaga MP, Brush SR, Vaughan DK, Henry KR, Conley S, Naash MI, Swaroop A, Anderson RA (2007)

Fatty acid composition of photoreceptor membranes of Nrl -/- mice and ground squirrel (S. tridecemlineatus)

retinas. ARVO Fort Lauderdale, Florida.

7. Conley SM, Fliesler SJ, Naash MI (2007) Isoleucine deletion at position 255/256 in the mouse opsin gene

causes an early onset blindness in transgenic mice. ARVO Fort Lauderdale, Florida.

8. Chakraborty D, Fliesler SJ, Naash MI (2007) The Role of Rds oligomerization in cone outer segment

assembly and maintenance. ARVO Fort Lauderdale, Florida.

9. X. Cai, B.A. Fliesler, M.J. Cooper and M.I. Naash (2007) Partial rescue of Rds haploinsufficency phenotype

with DNA nanoparticles. ARVO Fort Lauderdale, Florida

10. M.I. Naash, S.M. Conley, M.J. Cooper, X. Cai (2007) Functional rescue of the Rds haploinsufficency

phenotype with non-viral compacted DNA nanoparticles. !! Annual Vision Research Conference, May 4-5,

Fort Lauderdale, FL.

11. Naash MI (2006) DNA Compacted Nanoparticles as a Non-Viral Ocular Gene Transfer Therapy for

Hereditary Retinal Degeneration. XII International Symposium on Retinal Degeneration, October 23-28,

2006, San Carlos de Bariloche, Argentina.

12. Ding X-Q, Fitzgerald JB, Quiambao A, and Naash MI (2006) Cone Cyclic Nucleotide-Gated

Channel in Neural Retina Leucine Zipper Knockout, a Cone-Dominant Retina. XII International

Symposium on Retinal Degeneration, October 23-28, 2006, San Carlos de Bariloche, Argentina.

13. Chakraborty D, Ding X-Q, Skaggs J, Quiambao A, Fliesler SJ, and Naash MI (2006) Oligomerization of Rds

takes place in the photoreceptor outer segment: evidence from multiple mouse models. XII International

Symposium on Retinal Degeneration, October 23-28, 2006, San Carlos de Bariloche, Argentina.

14. Naash MI, Ballard M, Farjo R, and cai X (2006) Efficient Non-viral Ocular Gene Transfer with Compacted

DNA Nanoparticles. XVII International Congress of Eye Research, October 29-November 3, 2006, Buenos

Aires, Argentina.

15. J. Skaggs, R. Farjo, A.B. Quiambao, and M.I. Naash. (2006) The Major Intrinsic Protein of the lens

(Aquaporin-0) is expressed in the murine retina. ARVO Fort Lauderdale, Florida.

16. B. Quiambao, Z. Nash, B. A. Nagel, S. J. Fliesler and M. I. Naash. (2006) Evaluation of a macular dystrophy

genetic mutation in a cone-dominated murine retina. ARVO Fort Lauderdale, FL

17. R.Farjo, A.B. Quiambao, G.Moiseyev, J.-X.Ma, M.I. Naash (2006) Identification Of Genes Responsible For

The Maintenance And Support Of A Novel Cone Outer Segment Structure Lacking Lamellae. ARVO Fort

Lauderdale, Florida

CURRICULUM VITA FOR MUNA I. NAASH, PHD 7


18. Muna I. Naash, Ph.D., Mark J. Cooper, MD, Rafal Farjo (2006) Non-viral gene therapy for macular

dystrophies caused by mutations in RDS. The 29 th Annual Macula Society Meeting, Feb. 22-25, 2006,

North San Diego, California.

19. M.I. Naash, R.M. Ballard, J. Skaggs, Z. Nash, A. Quiambao, M.J. Cooper, R. Farjo (2006) Non-Viral Ocular

Gene Transfer for Hereditary Retinal Degeneration. 9 th Annual Meeting of the American Society of Gene

Therapy. May 31 - June 4, 2006, Baltimore, MD.

20. Farjo R., Cooper M. J., Skaggs J., Quiambao A.B., Naash, M.I. (2005) Non-viral gene delivery for ocular

diseases with compacted DNA nanoparticles. American Society for Gene Therapy, Louis, MO, June 1-5.

21. Chakraborty D, Ding X-Q, Quiambao A.B, Nagel B.A., Fliesler S.J., Naash M.I. (2005) Evaluating the Role

of Cysteine at Position 150 in Rds on Subunit Assembly in Rods versus Cones. ARVO Fort Lauderdale,

Florida.

22. Nash Z., Elliott M., Ding X., Takemori N., Matsumoto H., Anderson R.E. and Naash M. (2005) Protein

Characterization of Triton Insoluble Detergent Resistant Membrane Fractions in Bovine Rod Outer

Segments. ARVO Fort Lauderdale, Florida.

23. Naash M. I., Cooper M. J., Skaggs J., Quiambao A.B., Farjo R. (2005) Non-viral gene delivery for ocular

diseases with compacted DNA nanoparticles. ARVO Fort Lauderdale, Florida.

24. Farjo R., Skaggs J., Nash Z., Quiambao A.B, Moiseyev G., Nagel B.A., Ding X., Ma J, Fliesler S. J., Naash,

M.I. (2005) Cone photoreceptor viability in the absence of RDS protein. ARVO, Fort Lauderdale, Florida.

25. Grimm C., Wenzel A., Samardzija M., Frigg, R, Naash, M.I., Remé C.E. (2005) Retinal degeneration in a

mouse model of retinitis pigmentosa: rpe65 as modifier gene and role of phototransduction, c-fos, caspase-

1 and prion protein in the degenerative process. ARVO, Fort Lauderdale, Florida.

26. Canola, K.B. Angénieux, B. Tekaya, M., Quiambao, A., Naash, M.I., Schorderet, D.F. Munier, F.L.

Arsenijevic, Y. (2005) Neurogenic potential of retinal precursor cells (Rpcs) transplanted into a model of

terminal stages of retinitis pigmentosa. ARVO, Fort Lauderdale, Florida.

27. Grimm, C., Wenzel, A., Samardzija, M., Hotop, S., Groszer, M., Naash, M.I., Gassmann, M., Remé, CE

(2004) Constitutive overexpression of human erythropoietin in the mouse retina: Protection against induced

but not against inherited retinal degeneration. ARVO, Fort Lauderdale, Florida.

28. Nour, M, Nagel, BA, Fliesler, SJ and Naash M.I. (2004) Supplementation of peripherin/rds rescues mutationassociated

rod and cone photoreceptor defects in transgenic mice. ARVO, Fort Lauderdale, Florida.

29. Farjo, R, Nour, M, Peterson, W, Naash, M.I. (2004) Retinal transcriptome analysis during the resolution of

retinal detachment accomplished by P2Y2 receptor agonist INS37217. ARVO, Fort Lauderdale, Florida.

30. Canola, K, Angénieux, B, Quiambao, A, Naash, M.I., Schorderet, DF, Munier, F, Arsenijevic, Y (2004)

Behaviour of retinal precursor cells transplanted into the subretinal space of normal and degenerated adult

mouse retina. ARVO, Fort Lauderdale, Florida.

31. Nour, M, Fliesler, SJ and Naash, M.I. (2004) Supplementation of peripherin/rds rescues mutation-associated

rod and cone photoreceptor defects in transgenic mice. ASGT, June 2-6, Minneapolis, Minnesota.

32. Naash, M.I., Nour M, Stricker H, Fliesler SJ, and Ding X-Q (2004) Supplementation of peripherin/rds

rescues mutation-associated rod and cone photoreceptor defects in transgenic mice. RD2004, Perth,

Australia.

33. Ding X-Q, Nour M, Ritter LM, Goldberg AFX, Fliesler SJ, Naash, M.I. (2004). Preferential cone-specific

defects associated with the expression of r172w mutant peripherin/rds. RD2004, Perth, Australia.

34. Ding X-Q, Stricker H and Naash, M.I. (2004) Critical role of the second intradiscal loop of peripherin/rds in

the protein-protein interaction. XVI International Congress of Eye Research (ICER), August 28, 2004 in

Sydney, Australia.

VI. TEACHING ACTIVITIES

1. Course participation:

University of Oklahoma Health Sciences Center

Title Course Number Role

Molecular genetics Module (2004-current) CELL-6331 Director

Fundamental Principles in Molecular Genetics CELL-6331 Instructor

GPiBS Journal Club (Molecular Genetics & Therapeutics), 2005 BMSC-5221 Director

GPiBS advisory meeting (2003-2006) BMSC Instructor

Molecular Virology Module Viruses and gene therapy (2001-current) M I 6303 Instructor

CURRICULUM VITA FOR MUNA I. NAASH, PHD 8


Medical Histology (Lecture) CELL-8121 Instructor

Gene Therapy: on the frontiers of molecular medicine OCNS 6503 Instructor

Molecular Virology Module [Parvovirus viruses as gene therapy vectors] M I 6303 Instructor

Neurobiology of Diseases, [Molecular Etiology of Retinitis Pigmentosa and

Age Related Macular Degeneration].

BMSC 6503 Instructor

Gene therapy and eye diseases OCNS 6503 Instructor

Cellular System II (Fundamentals of Viral Gene Therapy), 2001-2003 BMSC-6052 Instructor

Neurobiology of Diseases (Molecular Etiology of Retinal Diseases) BMSC-6503 Instructor

Zoology Course [Advanced Topics in the Molecular Biology of Human

Disease].

Zoo 4970, OU/Norman Instructor

Molecular Approaches to Blinding Diseases OCNS 6503 Instructor

Teaching at UIC

1993–2000 Ophthalmology Morning Resident lectures, Department of Ophthalmology and Visual

Sciences, University of Illinois at Chicago.

1993–2000 Annual Resident Alumni Day, Department of Ophthalmology and Visual Sciences,

University of Illinois at Chicago.

1996-1997 Lecturing graduate students from the Department of Molecular Genetics at UIC,

Methods in Genetics, course 503, Introduction to Transgenics and Knockouts.

1997-1998 Lecturing graduate students from the Department of Molecular Genetics at UIC,

Genetics 513, Structure and Function of Ribozyme.

1998-1999 Lecturing graduate students from the Department of Molecular Genetics at UIC,

Genetics 513, G-protein Receptors.

1993–1995 Molecular Biology lectures to Clinicians, Department of Ophthalmology and Visual

Sciences, UIC.

1986-1990 Teaching Assistant, Tutored first year courses for graduate students in the Department

of Biochemistry, Baylor College of Medicine.

2. Team Teaching

2003-present Molecular Genetic Module, Department of Cell Biology, OUHSC, Fall

2000-present Graduate Student Research Project, Department of Cell Biology, OUHSC, Fall, Spring,

and Summer

2001 Participate in Teaching “Advanced Topics in the Molecular Biology of Human Disease”

to the undergraduate Student at the Oklahoma University, Norman, OK

2000-2004 Participate in Team Teaching of Histology Course for the OUHSC Medical Student.

2001-present Participate in Team Teaching of the first year Curriculum of the OUHSC Graduate

Program in Biomedical Science.

2002-2003 Independent Study for Undergraduate Student, Rusty Montgomery, for Special

Research Project for M-Bio 3990 at OU, Norman, August.

Summer, 2002 Independent Study for Undergraduate Student, Ranna Nash for Special Research

Project for Bio 492, University of Nevada, Los Vagus.

2001-2002 Independent Study for Undergraduate Student, Adam Alli for Special Research Project,

OU, Norman.

1/1-9/31, 2002 Independent Study for Undergraduate Student, Tammy Mai for Special Research

Project, OU, Norman.

2002 Fundamentals of Gene Therapy, GPiBS Spring Semester Curriculum, OUHSC.

2001 Advanced Topics in the Molecular Biology of Human Disease, OU Norman.

Fall, 2000 Neurogenetics, Department of Molecular Genetics and Department of Psychiatry,

University of Illinois at Chicago.

1999 Molecular Genetic Course, G501, University of Illinois at Chicago, December 17 th .

Winter, 1999 Teaching Molecular Biology of Vision in the Department of Molecular Genetics,

University of Illinois at Chicago.

Winter, 1998 Methods in Modern Neuroscience (Bios 582 and Neus 582), UIC.

Fall/Winter, 1997 Independent Research for undergraduate student, (BIOS399), UIC.

Winter, 1997 Molecular Basis of Cell Growth and Differentiation, Department of Molecular Genetics,

UIC, (GM513).

CURRICULUM VITA FOR MUNA I. NAASH, PHD 9


1993-2000 Doctoral Thesis Research, Department of Molecular Genetics (GM599), UIC.

1993-2000 Research Methods in Genetics, Department of Molecular Genetics (GM503), UIC.

1995-2000 Doctoral Thesis Research, Department of Biological Sciences (BIOS 599), UIC.

1995-2000 Graduate Student Project Research, Department of Biological Sciences (BIOS 597),

UIC, Fall, Spring, and Summer.

1992-2000 Teaching Molecular Genetics of Retinal Diseases in the Residence Program at the

Department of Ophthalmology and Visual Sciences, UIC.

3. Other Teaching Activities

Mentoring Junior Faculty Members

2004-present Mentor for Dr. Maria Queimado, an Assistant Professor of the Department of

Otorhinolaryngology, on her project as part of the OUHSC-INBRE Center

Grant.

2005-2006 Mentor for Dr. Xi-Qin Ding, an Assistant Professor of Department of Cell

Biology, on the Vision COBRA application.

2003-2004 Mentor for Dr. Michel Ihnat, an Assistant Professor of Department of Cell

Biology, on the Vision COBRA application.

Visiting Scholars

1998-1999 Dr. Huijun Yang, Visiting Scientist from West China University of Medical

Science, Dept. of Anatomy.

Research Instructors and Research Assistant Professors

2001-2006 Alexander Quiambao, Research Instructor, University of Oklahoma Health

Science Center, Department of Cell Biology.

2001-2005 Dr. Xi-Qin Ding, Research Assistant Professor, University of Oklahoma Health

Science Center, Department of Cell Biology,

2002 Dr. Qi Zhang, Research Assistant Professor, University of Oklahoma Health

Science Center, Department of Cell Biology.

Postdoctoral Fellows

Shannon Conley, PhD University of Arizona, Tucson, AZ. 2006-present

Xue Cai, Ph.D. University of Tennessee, Department of Biochemistry and Cellular and

Molecular Biology, Knoxville, TN. 2006-present.

Dibyendu Chakraborty, Ph.D. University of Calcutta, India, Dept. of Biochemistry. 2002-present.

Yanqing Hu Ph.D. Hematology Research Institute, Xiangya Hospital, Central South

University, P.R. China (2002). 2007-2008

Olga Nikolaeva Russian Academy of Science, Sant-Petersburg, Russia, 2006-2007.

May Nour, Ph.D. University of Oklahoma, Oklahoma Center For Neuroscience, 2003-2004.

Lakshmi Thirumangalakudi Madras University, India, Dept. of Biochemistry. 2003-2004.

Milena Kuzmanovic, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1998-2000.

Ming Cheng, Ph.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1996-2000.

Tong Cheng, Ph.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1996-1997.

Nasser Qtaishat, Ph.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1996-2000.

Xiaoping Xu, M.D., Ph.D. Loyola University of Chicago, Dept. of Neurology, 1996-2000.

Yun Cao, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Science,

1996-1998.

Shihong Li, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Science,

1995-1998.

Ting-Huai Wu, Ph.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Science,

1994-1997.

Samir Alhasan, Ph.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Science,

1994-1995.

Min Wang, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Science,

1993-1995.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 10


Residents

Laura Piippo, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1999-2000

Bienvenido Castillo, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1998-2000

Elizabeth Donohue, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1993-1994

J. Patrick Rhode, M.D. University of Illinois at Chicago, Dept. of Ophthalmology and Visual Sciences,

1993-1994

Graduate Students

2007-present Adarsha Koirala, University of Oklahoma, Dept. of Cell Biology.

2007-2008 Junie Paula Warrington, University of Oklahoma, OCNS Graduate Program.

2003-2006 Rafal Farjo, University of Oklahoma, Dept. of Cell Biology.

2005-2006 Balaji Hariharasundaram, University of Oklahoma, Dept. of Cell Biology.

2001-2005 Heidi Stricker, University of Oklahoma, Dept. of Cell Biology.

2000-2004 May Nour, University of Oklahoma, Oklahoma Center For Neuroscience.

1999-2001 Khaldun Al-Khatib, University of Oklahoma, Dept. of Cell Biology.

1998-2000 Monica Baig, University of Illinois at Chicago, Dept. of Bioengineering.

1998-1999 Shuen Mei, University of Illinois at Chicago, Molecular Genetics.

1997-1999 Gerard Carandang, University of Illinois at Chicago, Dept. of Bioengineering.

1996- 1999 Chibo Li, M.D., University of Illinois at Chicago, Dept. of Biological Sciences.

1993-1997 Tong Cheng, University of Illinois at Chicago, Dept. of Molecular Genetics.

1995-1996 Luisa Roveri, M.Sc, Loyola University of Chicago, Dept. of Neurology.

1993-1995 Yoshinobu Goto, M.S., Loyola University of Chicago, Dept. of Neurology.

Rotating Graduate Students

Adarsha Koirala University of Oklahoma, Cell Biology, Oklahoma Center For Neuroscience,

2008.

Junie Paula Warrington University of Oklahoma, Oklahoma Center for Neuroscience, 2007.

May Nour University of Oklahoma, GPIBS, Oklahoma Center For Neuroscience, 2000.

Heidi Stricker University of Oklahoma, GPIBS, 2001.

Raniyah Ramadan University of Oklahoma, GPIBS, 2001.

Laura Hern University of Oklahoma, GPIBS, 2001-2002.

Raniyah Ramadan University of Oklahoma, GPIBS, 2002.

Anne Murray University of Oklahoma, GPIBS, 2004.

Daniel Gay University of Oklahoma, GPIBS, 2004.

William M Rice University of Oklahoma, GPIBS, 2005.

Stephen Farris University of Oklahoma, GPIBS, 2005.

Member of Graduate Students Thesis Committee

2007-present Adarsha Koirala OUHSC Chair

2007-2008 Junie Paula Warrington OUHSC Chair

2003-2006 Rafal Farjo OUHSC Chair

2005-2006 Erin Wheather OU Member

2005-2006 Balaji Hariharasundaram OUHSC Chair

2005-2006 Anne Murray OUHSC Outside Member

2001-2005 Heidi Stricker OUHSC Chair

1999-2001 Khaldun Al-Khatib UIC/OUHSC Chair

1998-2000 Monica Baig UIC Chair

1998-1999 Shuen Mei UIC Chair

1998-1999 Patrick Rhode UF Outside Member

1996- 1999 Chibo Li UIC Chair

1993-1997 Tong Cheng UIC Chair

1995-1996 Luisa Roveri Loyola Member

1993-1995 Yoshinobu Goto Loyola Member

CURRICULUM VITA FOR MUNA I. NAASH, PHD 11


Medical Students

1997 Kevin Parker, University of Illinois at Chicago, Medical School.

1994, 1995 Lalitha Garimella, University of Illinois at Chicago, Medical School.

1995 Mitchell J. Siegan, University of Illinois at Chicago, Medical School.

1994 Kristin Mock, University of Illinois at Chicago, Medical School.

1992-1993 Jonathan R. Pirnazar, University of Illinois at Chicago, Medical School,.

Undergraduate Students

Jessika Shackelford Bachelor in Zoology Biomedical Sciences Research Institute, OU, OK, 2006present

Ron Ballard Bachelor in Zoology, OU, OK, 2005-present

Ehsan Noor Bachelor of Biochemistry at University of Oklahoma, OK, 2007-2008

Scott Mitchell Biology, University of Central Oklahoma, OK, 2006-2007

Contessa Majors Langston University, Langston as part of INBRE Summer Research Program-

2006 for Undergraduate Students.

Cindy Farris Health Science, SWOSU, OK, 2005

Didier Nuno Southern Nazarene University, Biology 2005-2006

Zack Nash University of Oklahoma, Junior Pre-Med., 2004-2005

Jeff Skaggs University of Oklahoma, Biology and Chemistry, 2003-2006

Rusty Montgomery University of Oklahoma, Senior in, Biochemistry, 2003-2004

Nada Hessani Microbiology, Columbia State University, Louisiana, 2003-2004

Shree Booker Undergraduate Student from University of Oklahoma, Langston as part of BRIN

2004 Summer Program for Undergraduate Students.

Grace Hassan South Western Oklahoma State University, Part of the BRIN program from

June 4 through August 2, 2003.

Heidi A. Welter Loras College, Iowa, Part of the SURE program from June 4 through August 2,

2004.

Rusty Montgomery University of Oklahoma, Senior in, Biochemistry, August 2002-2003.

Tammy Mai University of Oklahoma, Senior in, Biochemistry, November 2001-2002.

Matthew Traxler University of Oklahoma, Senior in Microbiology/Chemistry, November 2001-

2002.

Sarah Albahadily University of Oklahoma, Freshmen, Biochemistry, May 2001-2003.

Harper Ryan University of Oklahoma, Junior, Microbiology/Pre-medicine, May 2001-2003.

Ae R Kim Bachelor in Chemistry and Biochemistry, University of Oklahoma, OK, 2000-

2002

Samer Zantout University of Oklahoma, Junior, Zoology Major, January 2001-2002.

Adam Alli University of Oklahoma, Junior, Microbiology Major, January 2001-2002.

Josie Phan University of Oklahoma, Bachelors in Zoology, graduated May 2001, January

2001-August, 2001.

Sara Max Chemistry and Biology, Magna Cum Laude, Wartburg College, Waverly, Iowa,

2000-2001

Chris Kapetanopoulos Molecular Biology, Loyola University of Chicago, Dept. of Microbiology and

Immunology, 1998-2000

Maria Lee University of Illinois at Chicago, Junior, Education Major, 1999-2000.

Chirag Dholakia University of Illinois at Chicago, Senior Pre-Med., Kinesiology Major, 1998-

2000.

Sara Khan University of Illinois at Chicago, Junior Pre-Med., Biology Major, 1998.

Carolin Colon University of Illinois at Chicago, Junior Pre-Med., Biology Major, 1998.

Katarina Nedeljkovic University of Illinois at Chicago, College of Pharmacy, Junior. 1997-2000.

Xuemei Wang University of Illinois at Chicago, College of Pharmacy, Junior, 1997.

Wells Wheeler Colby College, Senior Pre-Med., Biology Major, 1997.

Shuqing Li University of Illinois at Chicago, College of Engineering, 1997.

Nancy Ziroli Emory University, Atlanta, Georgia, Junior and Senior Pre-Med. Biology Major,

1995 and 1996.

Paul Kogut Biology, University of Illinois Urbane-Champaign, IL., 1995-1996

Chester Gryczan Biology, Elmhurst College, Elmhurst, IL., 1994-1995

Maricza Collazo University of Illinois at Chicago, Chicago, IL, 1994-1997

Joni M. Sakurata Biology, University of Nebraska at Kearney, Kearney, NE, 1993-1994

Heather Grover Biology from Lehigh University, Bethlehem, PA, 1992-1993.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 12


Yasmin Kazzaz University of Houston, Junior Pre-Med., Biology Major, 1992.

Voe Cotton University of Columbia, Junior Pre-Med., 1991 and 1992 as part of the Summer

Medical and Research Training (SMART) Program at Baylor College of

Medicine in Houston.

High School Students

Reem Ibrahim Exchange student from Dubai, summer 2006

VII. PROFESSIONAL SERVICE

1. NIH Study Section and Other National and International Grant Review Panels

2006 Institute Clinique de la Souris (ICS) Mouse Clinical Institute (MCI), France

2002-2007 Member of NIH/NEI BDPE-C-NEI study Section.

2002 Ad Hoc reviewer for VIS C-February.

2001-2002 College of Medicine Alumni Association Grant at OUHSC.

2001-2002 Presbyterian Health Foundation, Bridge Grant & for Seed Grant at OUHSC.

2001-2002 COMAA Foundation at OUHSC.

1994-2000, 2004 The Foundation Fighting Blindness, Hunt Valley, MD.

1993, 1995, 1998-2000 Campus Research Board at UIC, Chicago IL.

1999 The Wellcome Trust, London, England

2000 COMITATO TELETHON FONDAZIONE ONLUS, Italy.

2. Editorial Services

Ad Hoc editorial member and reviewer for Investigative Ophthalmology and Visual Sciences

Reviewer for Journal of Biological Chemistry

Reviewer for Molecular Vision

Reviewer for Human Gene Therapy

Reviewer for Biochemical Journal

Reviewer for Human Molecular Genetics

Reviewer for Experimental Eye Research.

Reviewer for Visual Neuroscience.

Reviewer for Current Eye Research.

Reviewer for Vision Research.

Reviewer for Journal of the American Optometric Association.

Reviewer for Archives of Ophthalmology.

Reviewer for Gene

Reviewer for Journal of Comparative Neurology

Reviewer for Journal of Neuroscience

Reviewer for Genomics

Reviewer for Molecular Cell Biology

Reviewer for Biochemistry

3. Service to Professional Societies

Biochemistry Section Program Planning Committee for the Annual Meetings of the Association for

Research in Vision and Ophthalmology (ARVO), 2007-2010.

Abstract Coordinating Reviewer for the DNA Vectorology Section for the American Society of Gene

Therapy, 2008.

Program Organizer for Non-Viral Therapy Sessions at the American Society for gene Therapy

Symposium, 2007.

Chairing ADRP and RNA interference/Nanoparticles session in the Eleventh Annual Vision Research

Conference on Retinal degeneration and gene Therapy Pre ARVO Symposium, May 4-5, 2007, Ft.

Lauderdale, FL

Moderator for Hereditary Diseases Platform Session at the 29 th Annual Macular Society Meeting, Feb

22-25, 2006, North San Diego.

Moderator for Gene Therapy Platform Session at the Association for Research in Vision and

Ophthalmology (ARVO), 2005, Fort Lauderdale, Florida.

Moderator for Session at the Retinal Degeneration Symposium, 2004, Perth Australia.

Moderator for Sessions at the Association for Research in Vision and Ophthalmology (ARVO), 1998-

2002, Fort Lauderdale, Florida.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 13


Mouse Retinal Degenerations Symposium and workshop at the Jackson Laboratory, Bar Harbor, Maine,

September 26-29, 1999

Moderator for Sessions at the International Symposium of Eye Research (ICER), 1994

4. Invited Talks and Meetings

1. Invited to participate at the International Society for genetic Eye Diseases and Retinoblastoma (ISGEDR),

August 28-30, Strasbourg, France.

2. Invited to participate at the Symposium on the Nanotechnology for Drug Delivery and Tissue Bioengineering

in association with ARVO, May, 2008.

3. Invited to present at Department of Psychological & Brain Science, University of Louisville, Louisville, KY

October 15-17, 2007. Title: Non-viral ocular gene therapy.

4. Invited to present at the University of Illinois at Chicago, department of Ophthalmology and Visual Sciences,

October 12, 2007. Title: Nano-based non-viral gene therapy for ocular diseases.

5. Invited to present at the University of Missouri-Kansas City School of Medicine, department of

Ophthalmology and Visual Sciences, February 8-9, 2007. Title: Differential role for Rds in rods versus

cones outer segment morphogenesis.

6. Invited to Chair a session (adRP and RNA interference/Nanoparticles) and present at the pre ARVO

symposium on Retinal Degeneration and Gene Therapy, Eleventh Annual Vision Research Conference,

May 4-5, 2007, Convention Center, Ft. Lauderdale, FL.

7. Invited to present at the Biology and Chemistry of Vision, Federation of American Societies for Experimental

Biology (FASEB). Snowmass Village, Colorado, June 16-21, 2007.

8. Invited to participate at the Nanotechnology Session at ICER, 2006: Title: Non-viral Gene Delivery for

Ocular Diseases using DNA-Compacted Nanoparticles.

9. Invited to speak on RDS gene at the University of Pennsylvania, School of Dental Medicine, June 28 th ,

2006.

10. Invited to participate at the Inherited Maculopathies course in associated with ARVO, 2006. Title: Animal

Model of Pattern Dystrophy: Cone vs Rod influences.

11. Invited to participate at the Symposium on the Nanotechnology For Drug Delivery and Tissue

Bioengineering in association with ARVO, 2006. Title: Applications of Nanotechnology to Gene Delivery in

Ophthalmology.

12. Invited to participate at the 29 Annual Macula Society Meeting, Feb 22-25, 2006, North San Diego. Title:

Non-viral gene therapy for macular dystrophy caused by mutations in RDS.

13. Invited to participate in an interdisciplinary ARVO, entitled, “Nanotechnology For Drug Delivery and Tissue

Bioengineering,” during May 2006 at the Fort Lauderdale Convention Center.

14. Invited speaker at Johnson & Johnson Internal Ventures, Radnor, PA to present our Stem Cell therapy

results in rescuing visual loss in models of ocular diseases, January 12, 2006.

15. University of Arizona, Department of Ophthalmology and Vision Science, Tucson, AZ, Nov 8-10, 2005, Title:

The diverse role of Rds in rod versus cone outer segment morphogenesis.

16. Invited speaker at the Wednesday Cell Biology/Biochemistry joint Seminar Series at OUHSC, Sept., 21,

2005, Title: The Role of Rds in Rod and Cone Photoreceptors.

17. INSPIRE Pharmaceutical, Inc, Durham, NC, August 25-26, 2005, Title: Role of Rds gene in rods and cones.

18. American Society for Gene Therapy, Louis, MO, June 1-5, 2005, Title: Non-viral gene delivery for ocular

diseases with compacted DNA nanoparticles.

19. Department of Pharmacological & Physiological Science, Saint Louis University School of Medicine, St.

Louis, MO, USA, March 29 th , 2005, Title: Studies Toward the Role of Peripherin/Rds in Rods and Cones.

20. Department of Pharmacology and Neuroscience, University of North Texas Health Science Center at Fort

Worth, TX, April 5 th , 2005, Title: Peripherin/Rds Role in Outer Segment Morphogenesis of Rods and Cones.

21. Association for Research in Vision and Ophthalmology, May 2005, Fort Lauderdale, FL, Title: Non-viral

gene delivery for ocular diseases with compacted DNA nanoparticles.

22. American Society for Gene Therapy, June 1-5, 2005, Louis, MO, Title: Non-viral gene delivery for ocular

diseases with compacted DNA nanoparticles.

23. XIth International Symposium on Retinal Degeneration, Perth Australia, 2004, Title: Supplementation of

Peripherin/rds Rescues Mutation-Associated Rod and Cone Photoreceptor Defects in Transgenic Mice.

24. University of Barcelona, Department of Genetics, Barcelona, Spain, 2002, Title: Disease Causing Mutations

in the Peripherin/rds Gene: transgenic mouse studies.

25. Xth International Symposium on Retinal Degeneration, Burgenstock, Switzerland, 2002, Title: Correlation of

rate of degeneration with the location of the mutated amino acid in opsin: a transgenic mouse study.

26. October Hot Topics Seminar Series for Oklahoma Center for Neuroscience, University of Oklahoma Health

Science Center, Oklahoma City, OK, On Molecular Approaches to Blinding Diseases, October 18, 2001,

CURRICULUM VITA FOR MUNA I. NAASH, PHD 14


Title: Transgenic models to study the mechanism of visual loss associated with mutations in photoreceptor

specific genes.

27. IX International Symposium on Retinal Degeneration, Durango, CO, 2001, Title: Animal Models of Human

Retinal Diseases and the Application of Gene Therapy.

28. Laboratory of Retinal Cell Biology, University Hospital Zurich, Zurich, Switzerland, Sept. 25, 2001, Title:

Gene therapy in animal models of Retinal Diseases: problems and prospects.

29. Vision Club Meeting at The Dean A. McGee Eye Institute, Dept. of Ophthalmology, University of Oklahoma

Health Science Center, Oklahoma City, OK, May 8, 2001, Title: Ribozyme therapy in animal models of

Retinal Diseases.

30. University of Colorado Human Medical Genetic Program Seminar Series, April 12, 2001, Title: Ribozyme

therapy in animal models of Retinal Diseases.

31. The Macular Society Meeting (24 th Annual), Scottsdale, AZ, February 23-26, 2000, Title: Ribozyme therapy

in transgenic model of congenital stationary night blindness

32. Foundation Fighting Blindness, Regional Meeting, Dallas, TX, October 29, 2000, Title: Application of gene

therapy in animal models of retinal degenerative disorders.

33. Oklahoma Center for Neuroscience, University of Oklahoma Health Science Center, Oklahoma City, OK,

Decade of the Brain, September 29, 2000, Title: Animal models of human retinal diseases and the

application of ribozyme therapy.

34. The Macular Society Meeting (23 rd Annual), Rio Grande, Puerto Rico, February 23-26, 2000, Title:

Transgenic model of cone degeneration and the application of gene therapy

35. Annual Research Symposium on Genetics in Clinical Research, Seattle, WA, December 9-13, 1999, Title:

Animal models of human retinal diseases and the application of gene therapy

36. Mouse Retinal Degenerations Symposium and workshop at the Jackson Laboratory, Bar Harbor, ME,

September 26-29, 1999, Title: Transgenic models of retinal degeneration due to mutations in peripherin/rds

gene

37. Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, 1999,

Title: The role of peripherin/rds gene in human retinal diseases.

38. Department of Ophthalmology, University of Michigan, An Arbor, MI, 1999, Title: Animal models of human

retinal diseases.

39. Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, OK, 1999, Title:

Animal models of human retinal diseases and the application of ribozyme therapy.

40. Department of Medicine, University of Illinois at Chicago, Chicago IL, 1999, Title: Functional abnormalities

associated with various mutations in the rhodopsin gene: Studies in transgenic mice.

41. Department of Ophthalmology, Saint Louis University Eye Institute, Saint Louis University Health Sciences

Center, Saint Louis, MO, 1998, Title: The effect of peripherin/rds haploinsufficiency on rod and cone

photoreceptors.

42. Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL, 1998, Title:

Animal models of human retinal diseases: studies on transgenic mice.

43. Department of Pharmacology and Toxicology, University of Milwaukee, WI, 1998, Title: Transgenic mice

and retinal diseases.

44. Department of Neurology, University of Illinois at Chicago, Chicago, IL, 1997, Title: Gene therapy for retinal

degeneration.

45. VII International Symposium on Retinal Degeneration, Sendai, Japan, 1996, Title: Functional abnormalities

associated with various mutations in the rhodopsin gene: studies on transgenic mice.

46. XII International Congress of Eye Research, Yokohama, Japan, 1996, Title: Light-Induced acceleration of

photoreceptor degeneration in transgenic mice expressing mutant rhodopsin.

47. Department of Ophthalmology, University of California San Francisco, San Francisco, CA, 1996, Title:

Photoreceptor degeneration associated with the expression of mutant opsin gene.

48. Alcon Laboratories, Fort Worth, TX, 1996, Title: Functional abnormalities in transgenic mice expressing a

mutant rhodopsin gene.

49. University of Waterloo, School of Optometry, Ontario, Canada, 1996, Title: Polygenic disease and retinitis

pigmentosa: albinism exacerbates photoreceptor degeneration induced by the expression of a mutant opsin

in transgenic mice.

50. Department of Ophthalmology, Louisiana State University, New Orleans, LA, 1996, Title: Functional

abnormalities associated with various mutations in the rhodopsin gene: studies on transgenic mice.

51. Cleveland Clinic Foundation, Cleveland, OH, 1996, Title: Animal models of human retinitis pigmentosa and

macular degeneration.

52. Merck Research Laboratories. West Point, PA, 1996, Title: Functional abnormalities associated with various

mutations in the rhodopsin gene: studies on transgenic mice.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 15


53. Department of Anatomy and Cell Biology, University of North Texas. Fort Worth, TX, 1996, Title: Transgenic

mice expressing different mutations in the opsin gene simulate different forms of human autosomal

dominant retinitis pigmentosa.

54. XI International Congress of Eye Research, New Delhi, India, 1994, Title: Functional and molecular studies

of a transgenic mouse model for human ADRP.

55. XI International Symposium on Retinal Degeneration, Jerusalem, Israel, November 6-10, 1994, Title:

Characterization of transgenic mouse model expressing mutant rhodopsin gene.

56. Department of Genetics, University of Illinois at Chicago, IL, 1993, Title: Functional abnormalities associated

with the expression of mutant opsin gene: transgenic mouse studies.

57. Dean A. McGee Eye Institute, University of Oklahoma, Oklahoma, OK, 1992, Title: Transgenic mouse

models of human retinal diseases.

58. X International Symposium on Retinal Degeneration, Costa Smeralda, Sardinia, 1992, Title: Simulation of

ADRP by introducing mouse opsin mutations into transgenic mice.

59. Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, IL, 1991, Title: Lightexposure

induces ubiquitin conjugation and degradation activities in the rat retina.

60. IX International Symposium on Retinal Degeneration, Stockholm, Sweden, 1990, Title: Factors involved in

genetic regulation of light damage in photoreceptors.

61. IX International Congress of Eye Research, Helsinki, Finland, 1990, Title: Ubiquitin in light-exposed rat

retina.

62. FASEB Summer Research Conference on Ubiquitin, Saxton River, VT, 1989, Title: The retina has an active

ubiquitin-protein conjugation system.

63. VIII International Symposium on Retinal Degeneration, San Francisco, CA, 1988, Title: Factors affecting the

susceptibility of the retina to light damage.

VIII. ADMINISTRATION

At OUHSC

2001-present Member of the Mouse User Committee at OUHSC

2004-present Director of the Cell Biology Graduate Program at OUHSC

2004-present Chair of the Graduate Education Committee, Department of Cell Biology, OUHSC

2005-2006 Member of the Academic Misconduct Board Pool-College of Medicine

2004-2005 Member of the SWAT Committee

2004-2005 Member of the Diabetes, Obesity, and Metabolic research group for the SWOT Analysis

2003-2006 Member of the GPiBS Advisory Committee

2000-2005 Tenure and Promotions Committee, Department of Cell Biology, OUHSC

2003-2004 Member of the VP Committee on Obstacles to Technology Development on the

OUHSC Campus

2001-2004 Summer Undergraduate Research Committee (SURE)-Selection Committee at OUHSC

2001-2004 Member of the Adjunct Faculty Committee in Cell Biology, OUHSC

2000-2003 Member of the GPiBS Recruitment Committee at OUHSC

2001-2002 Member of the Academic Misconduct Board Pool-College of Medicine

At UIC

1996-2000 Member of the Public Awareness Sub-Committee at the Dept. of Ophthalmology and

Visual Sciences at UIC

1996-2000 Co-director of the Structural and Imaging Module of the NEI Center Grant at the Dept.

of Ophthalmology and Visual Sciences at UIC

1996-2000 Member of the External Relation Committee at the Dept. of Ophthalmology and Visual

Sciences at UIC

1996-1999 Chair of Grants and Awards Committee at the Dept. of Ophthalmology and Visual

Sciences at UIC

1992-2000 Member of the Faculty Advisory Committee at the Dept. of Ophthalmology and Visual

Sciences at UIC

1992-2000 Member of the Research Committee at the Dept. of Ophthalmology and Visual

Sciences at UIC

IX. MEMBERSHIPS, HONORS, AWARDS and SPECIAL

RECOGNITION

1. Scholarly, Professional and Membership of Societies

2004-present American Society for Gene Therapy (ASGT)

CURRICULUM VITA FOR MUNA I. NAASH, PHD 16


2002-present American Society for Cell Biology

1998-present The Macular Society

1984-present Association for Research in Vision and Ophthalmology (ARVO)

1988-present International Congress for Eye Research (ICER)

1988-present Retinal Degeneration Symposium (RD)

1988-present Federation of American Societies for Experimental Biology (FASEB)

1993-2000 Molecular Biology Retreat at UIC

1992-present American Association for Advancement of Science (AAAS).

1992-2000 American Society for Biochemistry and Molecular Biology

1992-2000 American Society for Neurochemistry

2. Awards, Honors, Special Recognition

2007 Nominated for the OUHSC George Lynn Cross Research Professorship Award.

2006 Nominated for the OUHSC George Lynn Cross Research Professorship Award.

2005 Voted one of “50 Woman Making a Difference in Oklahoma in 2005” and Nominated for

2005 Woman of the Year (Oklahoma).

2005 Awarded Senior Faculty Award at OUHSC-2005.

1996-8 Illinois Eye Fund Travel Award, Dept. of Ophthalmology and Visual Sciences to attend

the Association for Research in Vision and Ophthalmology, Fort Lauderdale, Florida.

1996 Faculty Foreign Travel Award at UIC to attend the XII International Symposium of Eye

Research, Yokohama, Japan.

1989 Western Eye Research Travel Award, Timberline Lodge, Oregon.

1988 Eighth International Congress of Eye Research Travel Award, San Francisco,

California.

1988 The V.C. Joshi Memorial Award for the best presentation from Baylor College of

Medicine, Dept. of Biochemistry, Houston TX.

3. Fellowships and Supplemental Training

1990-1992 Postdoctoral Fellowship from NIH/NEI (Individual NRSA, #EY06330) on the “Mouse

models for retinal degeneration.” Baylor College of Medicine, Houston, Texas.

1990 Postdoctoral Fellowship from the Foundation Fighting Blindness, Dept. of

Ophthalmology Core Grant, Baylor College of Medicine, Houston, Texas.

1986-1990 Predoctoral Fellowship from the NIH/NEI training grant #EY-07001 on the

“Characterization of ubiquitin system in the rat retina.” to Dept. of Ophthalmology,

Cullen Eye Institute, Baylor College of Medicine, Houston, Texas.

X. OTHER

Approved Contracts and Commercial Research

Approved contract with ACUCELA INC for the use of their drug on mice.

Approved contract with JOHNSON & JOHNSON for the use of their stem cells on our P23H model of

retinitis pigmentosa.

Approved contract with NOVARTIS PHARMACEUTICALS, INC for the use of anti-apoptotic drug on our

P23H model of retinitis pigmentosa.

Approved contract with INSPIRE PHARMACEUTICALS, INC for the use of certain drug trials for gene

therapy experiments on our transgenic models for retinal diseases.

Approved MTA with COPERNICUS THERAPUETICS, INC for the use of DNA compacted nanoparticle

technology to enhance drug delivery to photoreceptors and retinal pigment epithelium of animal

models of ocular diseases.

Approved MTA with Dr. Mark Kay at Stanford University on the use of Sleeping Beauty motif for

transposone transposase activity to enhance genomic insertion of non-viral therapy to

photoreceptors and retinal pigment epithelium of animal models of ocular diseases.

Approved contract for joint study with the University of Florida on performing gene therapy trials on

R172W and C214S mutant peripherin/rds transgenic models for cone specific dystrophy and

Autosomal Dominant Retinitis Pigmentosa, respectively.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 17


XI. Research Interests

1. Disease Mechanisms Associated with Mutations in Photoreceptor-Specific Tetraspanin proteins

(Rds & Rom-1). A main focus of my research is to characterize the functional role of photoreceptor specific

tetraspanin proteins, retinal degeneration slow (Rds) and rod membrane protein-1 (Rom-1), in outer

segment (OS) morphogenesis and maintenances. We are interested in identifying Rds partners and the

molecular abnormalities associated with disease causing-mutations. Without this protein, the OS are not

formed in mice and over 80 different mutations so far have been identified in patients to associate with

several forms of inherited retinal diseases. Proteomics, molecular biology, protein biochemistry, tissue

culture, transgenic and knockout technologies are employed to test the hypothesis that Rds complex is

different in composition between inner and OS compartments, as well as between rod and cone OS regions.

We are investigating the functional involvement of several interacting proteins such as the cone cGMP

gated channel (CNG6) with the Rds complex. We are also investigating Rds/Rom-1-primary or -auxiliary

complex formation associated with diseases-causing mutations in Rds & Rom-1. We are also interested in

identifying the check points in Rds trafficking in rods and cones.

2. Mechanisms of Photoreceptor Cell Death Associated with Retinal Detachment, Pharmaceutical

interventions and Ocular Drug Therapy. Our goal is to identify novel molecular targets for treating retinal

detachment and macular edema by gene expression profiling in the absence and presence of a

pharmaceutical compound (INS37217) which has previously been shown to accelerate the reattachment

process. We have developed retinal detachment model in our lab and used Affymetrix MOE430 GeneChips

with qRT-PCR, along with Western blotting to identify genes that are modulated at different times post

detachment. We have identified several genes involved in the process of retinal detachment and others

involved in accelerating reattachment. We are currently evaluating the roles of these genes in normal retinal

function. For further pharmaceutical applications, we have been using retinal detachment model for drug or

cell delivery to the subretinal space to test their efficacy in protecting the retina from the ongoing

degeneration. We have optimized this technique for mice at ages ranges from young (postnatal day 1-5) to

adult (postnatal day 30 or older). We are currently sponsored by several pharmaceutical drug companies to

test the anti-apoptotic effect of their drugs, rescue of degeneration by delivering genetically modified stem

cells, or using drugs to enhance retinal attachment by removing the extra fluid from the subretinal space.

We are testing these agents in our models of retinal diseases.

3. Development of Non-viral Ocular Therapy. A second main focus of our research is related to the

development of therapeutic interventions to combat loss of vision in several animal models of ocular

diseases. We are using self-compacted DNA nanotechnology, mini-circle vectors, self-replicating vectors,

and helper-independent Sleeping Beauty Trasposon-Transposase vectors to develop nonviral and effective

therapeutic strategies for ocular diseases; particularly those associated with loss-of-function mutations in the

retina and retinal pigment epithelium (RPE), diabetes, glaucoma, wound healing, cancer and others. We

are currently evaluating the safety and efficiency of gene transfer delivery and expression using our

nanotechnology with circular versus linear vectors expressing GFP and directed by photoreceptor and RPE

specific promoters. While a number of pre-clinical studies have achieved the rescue of animal models of the

retina and RPE diseases using virally-mediated gene transfer, viral delivery poses a number of complicating

factors. Aside from the limitations of viral tropism which restricts gene delivery to cell types, clinical studies

have questioned the safety of viral vector used in gene therapy. As a result, a need exists for an effective

and well-tolerated method of therapeutic delivery to ocular tissues. Our studies will develop non-viral

vectors that can be compacted up to 20kd in size to less than 8nm in diameter for efficient delivery to the

nucleus (less than 15 min). Because these nanoparticles are so small and condensed, they can pass safely

through cell membrane and into the nucleus. Our experimental plan requires three components: engineered

nanparticles for efficient and direct delivery of therapeutic agent to the nucleus, genetically engineered

therapy, and animal models of ocular diseases, all of which are currently available in our laboratory.

4. Proteomic Analysis of Photoreceptor Outer Segment Raft Proteins. Another area of interest is to

identify photoreceptor proteins associated with detergent resistant membrane domains isolated from purified

rod outer segments. Our initial goal is to identify proteins that associate with raft domains in light dependent

manner and to evaluate the role of raft in photoreceptors. To accomplish this goal we are utilizing mass

spectrometric methods together with sub-fractionation techniques, western blotting, immunocytochemical

techniques and affinity chromatography methods.

5. The role of aquaporin family in diabetes and obesity. Recently, we found that Aquaporin 0 (AQP0), a

plasma membrane protein involved in water homeostasis that was previously thought to be lens-specific, is

CURRICULUM VITA FOR MUNA I. NAASH, PHD 18


also expressed in bipolar cells and that AQP0-deficient mice lack cone-derived b-wave amplitude in the

electroretinogram (ERG). We then evaluated the role of AQP0 in bipolar cells by studying the structural and

functional consequences of its absence. We used Cat Fr mice for these studies. These mice are considered

functional AQP0 knockout mice. We recently documented that AQP0 is expressed by rod and cone ON

bipolar cells and that in the absence of functional AQP0, the synaptic transmission from cone

photoreceptors to cone ON bipolar cells is selectively disrupted. However, we found that the synaptic

contacts between cones and cone OFF bipolar cells and between rods and rod ON bipolar cells are normal.

At the ultrastructural level, the outer plexiform layer in Cat Fr mice is disorganized with aberrant accumulation

of membranous material in the dendritic processes. This observation is novel and exciting and suggests a

role for AQP0 in synapse formation. Our future goals are to determine precisely what role AQP0 plays in

regulating synapse formation and/or dendritic growth by cone ON bipolar cells and to identify why the lack of

functional AQP0 does not appear to disrupt the synapses of rod bipolar cells, which also express AQP0.

Another observation is that these mice suffer from age-related obesity and might suffer from diabetes.

CURRICULUM VITA FOR MUNA I. NAASH, PHD 19

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