Download Final Presentation

csep.cnsi.ucsb.edu

Download Final Presentation

Characterizing Interactions ofChemical Penetration Enhancerswith SkinMoon Jean HoChemistry, Pasadena City CollegeMentor: Pankaj KarandeFaculty Advisor: Samir Mitragotri


Understanding the ChemistryQuantifying the effects of CPEs on stratum corneumStratum CorneumEpidermisDermis15 µm mLipid BilayersCorneocytesHair FollicleIn vitro:Stratum corneum (SC) of porcine skin


Fourier Transform Infra RedSpectroscopy (FTIR)1 2 3 4Isolate stratumcorneumFTIR attime zeroSoak& rinseFTIR at24 hr


FTIR Spectrum of Porcine SC4.24.03.8ProteinsAbsorbanceAbsorbance3.63.43.23.02.82.62.42.22.01.81.61.41.21.00.80.60.4Lipids4000350030002500200015001000500Wavenumbers (cm-1)Wavenumber (cm -1 )


FTIR Spectrum of Porcine SCAbsorbanceAbsorbance4.24.03.83.63.43.23.02.82.62.42.22.01.81.61.41.21.00.80.60.44000N-Hstretching3500Amide:O=C NH 23000Lipid chains (CH 2)25002000Wavenumbers (cm-1)1500Wavenumber (cm -1 )C=O stretching1000C-N stretchingScissoring modesWagging modes500


Change in Peak Area0.20Absorbance0.100.002830 28502870Wavenumber (cm -1 )


SLS:DPC Phase Map2850 cm-1 1 peakFTIRConductivityConcentration (%)2.01.51.00.501Hotspot0.50.5Concentration (%)1 SLS0 DPC2.01.51.00.501Hotspot0.50.510Increasing area changeWeight FractionWeight FractionKarande & Mitragotri, Pharm. Res. 2002


AcknowledgementsKaitlin Ergun (Undergraduate, UCSB)Amit Jain (Post Doctoral Fellow, UCSB)

More magazines by this user
Similar magazines