Etudes sur le mécanisme de remodelage des nucléosomes par ...

tel-00413908, version 1 - 7 Sep 2009
Index of Figures and Tables
Figure I.1 Nucleosomes: Beads-on-a-string model…………………………………….20
Figure I.2 Histone fold of the core histones…………………………………………….21
Figure I.3 Nucleosome assembly……………………………………………………….22
Figure I.4 Structure and potential position of linker histone on nucleosome…………..23
Figure I.5. Structure of the 147 bp nucleosome core particle at 1.9 Å resolution……...25
Figure I.6 Different orders of chromatin architecture……………………………………….26
Figure I.7 Models of 30 nm fiber organization…………....……………………………27
Figure I.8 General Properties of euchromatin and heterochromatin……………………29
Figure I.9 Canonical histones and their variants………………………………………..31
Figure I.10 Phylogenetic tree of H2A variants………………………………………….33
Figure I.11 Sequence and structure of H2A.Bbd………………………………………..38
Figure I.12 Posttranslational Modifications of the Core Histones………………………40
Figure I.13. Classification of SNF2 family ATPases……………………………….…...44
Figure I.14 Classes of ATP dependent Chromatin remodeling enzymes………………..45
Figure I.15 Subunit compositions of SWI2/SNF2 family complexes…….……………..45
Figure I.16 Domain organizations of SWI/SNF subunits………….…………………….49
Figure I.17 Subunit compositions of ISWI subfamily members…………………………50
Figure I.18 Domain organization of ISWI subunits……………………………………...53
Figure I.19 Subunit composition of INO80 subfamily members………………………...54
Figure I.20 Subunit compositions of CHD subfamily members…………………………55
Figure I.21 Models for SWI/SNF recruitment to target genes…………………………...57
Table I.1 Biological functions of chromatin remodelers…………………………………62
Figure I.22 Structures of SWI/SNF and RSC complexes reconstructed from Cryo-Electron
micrographs……………………………………………………………………………….72
Figure I.23 Summary of various biochemical activities of ATP dependent remodelers.
……………………………………………………………………………………….......78
Figure I.24 Proposed models of nucleosome sliding by ATP dependent remodelers......81
Figure II.1. AFM visualization of RSC mobilized nucleosomes…………..………….90
Figure II.2. The initial step of the RSC nucleosome mobilization reaction is the generation of
a stable, non-mobilized particle containing 180-190 bp of histone octamer associated DNA.
…………………………………………………………………………………………91
Figure II.3. Electron Cryo-Microscopy (EC-M) of the RSC treated mono- and trinucleosomes
shows that different species are present in the RSC remodeling reaction.
………….………………………………………………………………………………95
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