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download PDF version - Dr Harold Hillman

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the research workers who dissected out and published papers on neuroglial clumps: H. Hydén; A.Pigon; A. Bäckman; I. Augustsson ; A. Hamberger; H. <strong>Hillman</strong>, P. Sartory, K. Deutsch, T.S.Hussain, J. Allen, I. Chughtai, D. Jarman, L. Hertz and others.Figure 5. Partially teased human caudate nucleus from a 70-year old male to show neuroglialnuclei and fine granular material. Phase contrast, x 700.In the unfixed state, the neuroglial clumps appear in 0.25 M sucrose, in Krebs-Ringersolution, in „199‟ medium or in normal saline, as intensely white, because they are made of avery large number of granules, mostly appearing 0.5-2 µm in diameter (Figure 6). They are veryfriable and adhere together like pieces of rice pudding. Occasionally, neuron cell bodies can befound in the neuroglial material, and unattached nuclei (Figure 5) can be seen apparently movingaround the neuroglia. When teased apart, it can be seen that vast numbers of fine fibres coursethrough the neuroglia. Probably, most of these fibres are dendrites, and non myelinated fibres,with some myelinated fibres visible (<strong>Hillman</strong> and Jarman, 1991).We took pieces of brain and spinal grey matter, 2-3 mm in diameter, and sandwichedthem in similar-sized pieces of liver or kidney. We then stained the sandwiches with four stainsrecommended for neuroglia. In each case, they showed the membranes of the liver cells and23

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