download PDF version - Dr Harold Hillman

kidney tubules. However, in the nervous tissue we could see no continuous membrane betweenthose of the neurons and those of the naked nuclei. The next step was to examine theneurological clumps directly. Hydén and his school generally believed that the neurologicalclumps consisted of astrocytes or oligodendrocytes, although - as far as I could find - they didnot test them with the ‟specific‟ classical stains or with antibodies.We decided to look at the clumps directly. We took out individual clumps adjacent to cellbodies, and dissected out any neurons which might be present in them. Then we teased each ofthem and stained them directly with Janus-green neutral red, which shows up mitochondria, withwhich we also stained individual neuron cell bodies. Both the neuron cell bodies and the clumpswere seen to be full of aggregates of mitochondria (Figure 6).Figure 6. Fine granular material stained with Janus green-neutral red, viewed by phase contrastmicroscopy. m is mitochondria. The bar is 20 µm for a and b and 50 µm for c. a is an isolatedneuroglial clump; b has a neuroglial clump in the upper part of this picture, and a neuron cellbody in the lower, and c shows the clump separated to show that the fine granular material24