Conformation Sensitive Capillary Electrophoresis Conformation ...

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Universally tagged PCRM13F = tgt aaa acg acg gcc agtM13R = cag gaa aca gct atg accM13F tagGS1GS2M13R tagPCRTemplate DNAProductM13FM13RSequencingreaction
Assay Design: Standardised primeroptimisation and design specificationM13F tagFM13FFGS1M13F = tgt aaa acg acg gcc agtM13R = cag gaa aca gct atg accGS2M13RM13R tagPCRTemplate DNAProductF50bp buffer50bp buffer
Page 1: Conformation Sensitive Capillary El
Page 5: Batch exampleFrag 1 Frag 2Plate 1FF
Page 9 and 10: Assay Design: Standardised primerop
Page 11 and 12: Assay Design: Standardised primerop
Page 13 and 14: US1FBRCA2 Ex03GS1GS2[GS2] fmol/µl
Page 15 and 16: US1FBRCA2 Ex11EGS1GS2[GS2] fmol/µl
Page 17 and 18: Controls• Per fragment• Mutatio
Page 19 and 20: Mixed productsBC112BC111LBC21
Page 21 and 22: Special cases Homozygotes• May
Page 23 and 24: Dilution of productsDilution in wat
Page 25 and 26: Validation• CSCE setup using Sang
Page 27 and 28: Validation•Testing the Sanger pro
Page 29: AcknowledgementsCSCE• Chris Matto

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