Marie Curie Workshop Laboratory Manual Clostridial Gene ...

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Contaminated Biological WasteWaste Category Examples Disposal routeSolid wasteContaminated smallplasticsContaminated largeplasticstips, tubes andspreadersfalcon tubes‘sweetie’ jars, when full close,label with autoclave tape, place inautoclave bags and take to mediaprep for autoclaving.autoclave bags, when full close,label with autoclave tape, place inautoclave bags and take to mediaprep for autoclaving.Low-level contamination gloves, tissues yellow bags. When full place in thelarge yellow skip outside the mediaprep roomSolid growth media Petri dishes autoclave tins. When 2/3 full taketo media prep for autoclaving. Donot overfillLiquid growth mediaSmall volumes Eppendorfs sweetie jars. when full close, labelwith autoclave tape, place inautoclave bags and take to mediaprep for autoclaving.Larger volumesSterilins, falcontubes500ml Duran bottles. When 2/3full.Do not put chemical disinfectants(Trigene, etc) into thesecontainers as they can producehazardous vapours whenautoclavedGlass Universals orErlenmeyer flasksplace in dedicated boxes in lab.Cover open flasks with aluminiumfoil and secure with autoclave tape.Take full boxes to media prep forautoclavingDo not put chemical disinfectants(Trigene, etc) into thesecontainers as they can producehazardous vapours whenautoclavedUniversity of Nottingham, Sept ’06 Page 32
Mixed biological and chemical wasteWaste CategoryContaminated plasticsDisposal routesterilise chemically using Trigene or appropriatedisinfectant. Put into yellow bags for collection andincineration. DO NOT put mixed waste in autoclavebags due to the potential danger to the autoclaveoperative from chemical vapours.Contaminated glassware chemical sterilisation by immersion overnight in 1: 100Trigene solutions prior to washing. Inform the technicalstaff in the wash-up area of the nature of the mixedbiological/chemical hazard as they receive the glassware.Low-level contamination(gloves, tissues)Liquid growth mediayellow bags for collection and incinerationspeak to local safety monitors or the CSO for specificguidance on these routes.small volumes in an Eppendorf tube can be put into burnbins for incineration. It is always a good idea to put someform of absorbent material in burn bins that are expectedto contain liquid wasteLarger volumes (aspirants to traps, waste contained in amulti-well dish) should be separated from noncontaminatedchemical waste and processed followinglocally arranged routes considered in risk assessments.These should take account of methods to immobilise thechemical, the survival of the biological substance and theneed to inform others involved in the waste-processingroute.University of Nottingham, Sept ’06 Page 33
Page 1 and 2: Marie Curie Workshop Laboratory Man
Page 3 and 4: FOREWORDIt is with great pleasure t
Page 5 and 6: ProgrammeMonday 11 th September - A
Page 7 and 8: Overview of practical sessionsDurin
Page 9 and 10: 3. Perform PCR using the following
Page 11 and 12: Ligation of the digested PCR produc
Page 13 and 14: Wednesday 13 th SeptemberTODAYPerfo
Page 15 and 16: The thiamphenicol will select for t
Page 17 and 18: Wash cells and allow recovery perio
Page 19 and 20: 1. Label PCR tubesLabel 8 PCR tubes
Page 21 and 22: Inspect plates and interpret result
Page 23 and 24: PARTICIPANTSSURNAME FIRST NAME INST
Page 25 and 26: IntroductionThe Centre for Biomolec
Page 27 and 28: If you hear the fire/evacuation ala
Page 29 and 30: Good Laboratory PracticeWithin the
Page 31: Waste DisposalAll hazardous biologi

Marie Curie Workshop Laboratory Manual Clostridial Gene ...

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