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4 NEW ImageQuantLASTyphoon FLA 10.5 10.5 cm 16 bit tif jpg 100 240 V 50 60 Hz 200 W W H D30 57 28 cm 15 kgECLSelectLAS500 ImageQuant LAS 500 2,500,000ECL Select Western Blotting Detection Reagent for 1,000 cm 2 membrane RPN2235 50,000ImageQuant TL Software Package 1 DVD 28-9380-94 50,000ImageQuant TL 1-User License 1 28-9236-62 387,000Full-Range Rainbow Molecular Weight Markers 250 µl RPN800E 19,800 2,500,000Hybond-P20 20 cm 10 sheets RPN2020F 22,800ECL Gel Box 1 28-9906-08 13,200ECL Gel 10%, 10 wells 2 28-9898-08 3,00020123159282012929ECLSelectLAS5003045800GE1 ImageQuant LAS 500ECL SelectImageQuant TLFull-RangeRainbow MolecularWeight MarkersHybond-PECL Gel Box& ECL Gel190 122 www.gelifesciences.co.jp

14 1 3,000 15,000 ECLSelectLAS500 10 5 2.5 1.2 0.6 µg1:3,000 1 ECLSelectLAS500 1:5,0001:10,0001:15,000 Two-fold dilution of NIH/3T3cell lysate starting at 10 µgRabbit anti-pSTAT3 HRP-conjugated anti-rabbitIgG 1:100,000 : Amersham ECL PrimeBlocking 2% in PBS-T : Amersham Hybond-PImageQuantLASTyphoon FLA2ECLSelectLAS500 2 ECLSelectLAS500 ECL Advance 5 39 ng 20 19.5 ngECL Advance 5 2,500 ng1,250 ng625 ng313 ng156 ng78 ng39 ngECL Advance 20 ECL Advance2,500 ng1,250 ng625 ng313 ng156 ng78 ng39 ng19.5 ng39 ng19.5 ngNDGE1 39 ng 2 ECLSelectLAS500HeLa cell lysate ERK1/2 1:10,0001:100,000190 www.gelifesciences.co.jp123

43ECLSelectLAS500 Rainbow Molecular Weight Markers Rainbow Molecular Weight MarkersImageQuantLASTyphoon FLA 2 Rainbow Molecular Weight Markers 1 PAGE Deep PurpleSYBR GreenSYPRO RubyEtBr CBBGE1 Deep PurpleSYBR Green EtBr CBB190 124 www.gelifesciences.co.jp

ECLSelectLAS500 4 1 3 10 ECLSelectLAS500ImageQuantLAS ECLSelectLAS500 ImageQuant LAS4000mini ECLSelectLAS500 ImageQuant LAS 4000mini ECLSelectLAS500 1 3 4 JPG TIF ImageQuant LAS 4000mini ECLSelectLAS500 ECLSelectLAS500 ECLSelectLAS500 Rainbow Markers ECL SelectECL Select 2 3 30 1 ECL Select ImageQuant LAS 4000mini ECL Select Typhoon FLAECLSelectLAS500ImageQuant LAS 4000miniGE1 ECLSelectLAS500 ImageQuant LAS 4000mini ECLSelectLAS500 ImageQuant LAS 4000miniRainbow Molecular Weight Markers ECL Select Western Blotting Detection System190 www.gelifesciences.co.jp125

4ImageQuantLASTyphoon FLAGE1 CCD ImageQuant LAS 4010 ImageQuant LAS 4000 ImageQuant LAS 4000mini ECL ECL Prime ECL Select 312 nm UV EtBr Deep Purple 460 nm LED Alexa Fluor 488Cy 2SYBR Green I / IISYBR GoldSYPRO RubySYPRO OrangeFITCFAMEGFP ECFPAttoPhosPro-Q Emerald 488520 nm LED SYPRO RedCy3TAMRA5-ROXHEXAlexa Fluor 532/546/555Deep PurplePro-Q DiamondBODIPY 576/589R-phycoerythrinRFPHNPP630 nm LED Alexa Fluor 633/635Alexa Fluor 647/700Cy5BODIPY 650/665DiDTOTO 3DDAO Phosphate LED Silver,CBB,X-ray,film,NBT,BCIP 365 nm UV LED EtBrSYPRO RoseQdot 605/655/705/800710 nm LED Dy676Alexa Fluor 700/750630/520/460 nm / / LED EGFP 510DF10 filterLAS 28-9564-62190 126 www.gelifesciences.co.jp

4BAS Typhoon FLA 9500 Typhoon FLA 70003H 11 C 14 C 125 I 18 F 32 P 33 P 35 S 99m TC Macroarrayradiolabeled CyDye DIGE FluorsCy2 Cy3 Cy5 ECL Plex FluorsCy2 Cy3 Cy5 Protein StainsDeep Purple SYPRO Ruby NanoOrange ND NDPro-Q Diamond Pro-Q Sapphire 532 ELISAAttoPhos Cy3Cy5 Alexa Fluor 532/633 Nucleic acid stainsEtBr Vistra GreenSYBR GoldSYBR Green I / II PicoGreenRiboGreen ND NDECFAIkPhos Direct ECF DDAO Phosphate Cy2 Cy3 Cy5 FluoresceinFAMFITCAlexa Fluor 488 TETHEXROXTAMRA GFP ECL ECL Prime ECL Select NDImageQuantLASTyphoon FLAGE1 190 www.gelifesciences.co.jp127

4ImageQuant LAS 4010/4000/4000mini CCD F0.85 ImageQuant TL LAS 1000 LAS 4000 2011 6 LAS-1000LAS-1000Plus 2012 3 LAS-1000miniImageQuantLASTyphoon FLAX X ImageQuant LAS 4010 ImageQuant LAS 4010 X 1/2 3.1 ng Transferrin 2 SDS-PAGE -transferrin 1/10,000 -rabbitIgG-HRP1/50,000ECL Plus ImageQuant LAS 4010Tray Position160 secSensitivityHighHyperfilm-ECLX 60 secImageScanner III 200 100 50 25 12.5 6.25 3.1 Transferrin ng 200 100 50 25 12.5 6.25 3.1 Transferrin ngLAS-1000 LAS-1000 ImageQuant LAS 4000 LAS-1000 Anti-Mouse IgG-HRP Hybond-P ECL PlusLAS-1000 ImageQuant LAS 4010 10 GE1 190 ImageQuantLAS 40108 LAS-1000ImageQuant LAS 4010 ImageQuant LAS 4000 ImageQuant LAS 4000miniF0.85 43 mm CCD320 630-30-254465,53616 bitWDH mm 510480900 440370670kg 60 31ImageQuant TL128 www.gelifesciences.co.jp

Image Quant LAS 4000 ImageQuantLAS 4010LAS 3 UV 630 nm LED520 nm LED460 nm LED LED312 nm UV R670 Cy5575DF20 Cy3Y515605 EtBrUV 4ImageQuantLAS 4000 Ethidium Bromide LED312 nm UV 605 EtBr UV ImageQuantLASTyphoon FLAImageQuantLAS 4000mini LED CCD ImageQuant LAS 4010 with UV LED 11,200,000 ImageQuant LAS 4010 1 9,200,000Epi-IR Set (LAS) 710 nm LED 1 28-9589-23 1,200,000Epi-UV Set (LAS) 365 nmUV LED 1 28-9589-25 800,000EcoRepro 41 32 SyBR GreenSYPRORuby CCD ImageQuant LAS 4000 with LED 7,900,000 ImageQuant LAS 4000 1 6,500,000Epi-B Set (LAS) 460 nm LED 1 28-9589-19 800,000White Trans Set (LAS) 1 28-9589-18 600,000 EtBr CCD ImageQuant LAS 4000 6,500,000 CCD ImageQuant LAS 4000mini 4,300,000146 GE1 CCD ImageQuant LAS 4010 9,200,000 ImageQuant LAS 4010 Extended Warranty 2 ImageQuant LAS 4000 Extended Warranty 2 ImageQuant LAS 4000mini Extended Warranty 2 34 608,00034 560,00034 528,000IQ/OQ ImageQuant LAS 4010/4000 670,000190 www.gelifesciences.co.jp129

4ImageQuantLAS 4000/4010 ImageQuantLASTyphoon FLA ImageQuant LAS 4000/4010 ImageQuant LAS 4000mini 3 1 UV 21 ImageQuant LAS 4000 32 31&PC GFPCy2Cy3Cy5 Cy7GE1 3 IQ/OQ GFPCy2GFP GMP ImageQuant Cy2 Cy3 LAS 4010 Cy5 Cy7 Cy3 Cy5 Cy7 ImageQuant LAS 4000 IQ/OQ IQ/OQ 2 190 CCD 5 www.gelifesciences.co.jp 130 www.gelifesciences.co.jp

Typhoon FLA 9500 46 40 cm 3 473532635 nm ImageQuant TL Typhoon FLA 9500PMTPMTµm1025501002001,000cm46401550 µm2025 cm 5473 nm LD532 nm SHG635 nm LD65,536 16 bitWDH mm 900800400kg97 ImageQuant TLFLA Typhoon FLA 9500 2011 3 31 FLA-2000FLA-5000 2013 3 31 FLA-30004ImageQuantLASTyphoon FLA 2D DIGEAlexa 488Mab CHO fluorescene473 nmCy2532 nmCy3635 nmCy5530DF20Cy2570DF20Cy3665LPCy52D DIGE 134 Alexa Fluor 568 phalloidinAlexa Fluor 488wheat germ agglutinin 532 nm LPG 473 nm BPB110 µmAlexa 568 Typhoon FLA 9500 BGR with 2 16,440,000 Typhoon FLA 9500 BGR 1 13,500,000785 nm laser upgradeFLA 9500 1 1,800,000Typhoon FLA 9500 Extended Warranty 2 1,140,000 Typhoon FLA 9500 BGR with MA-PMT 2 14,640,000 Typhoon FLA 9500 BGR 1 13,500,000Typhoon FLA 9500 Extended Warranty 2 1,140,000EcoRepro 41 32 146 GE1 Typhoon FLA 9500 Extended Warranty 2 34 1,140,000 Typhoon FLA 9500 Extended Warranty Plus 2 34 1,254,000 Typhoon FLA 9500 1 34 850,000Extended Warranty Plus 114,000 2 560,000 190 www.gelifesciences.co.jp131

4RI Typhoon FLA 7000 20 25 cm 5 50 µm ImageQuant TL BAS Typhoon FLA 7000 2011 9 30 BAS-1000 1500BAS-2000BAS-3000BAS-1800 1800 IIImageQuantLASTyphoon FLATyphoonFLA 7000BGR PMT PMT(µm) 2550100200 2550100200(cm) 2040 20405525 µm2040 cm 25 µm2040 cm 5 5 473 nm LD 532 nm SHG 635 nm LD 650 nm LD 650 nm LD 65,536 16 bit 65,536 16 bit(WDHmm)940556360940556360(kg) 62 62ImageQuant TL25 cm520 cmIPImageQuant TL20 25 cm 50 µm 55 20 RNA RNA 32 P IPBAS-MS2040IP 650 nmB390S1000050 µm rIuD pseudouridinem3 3methylpseudouridine rluD CMC GE1 RI Typhoon FLA 7000 BGR with 2 12,192,000 Typhoon FLA 7000 BGR 1 10,800,000FLA Image Eraser BAS 1 28-9564-73 432,000Typhoon FLA 7000 Extended Warranty 2 960,000 BAS RI Typhoon FLA 7000 IP with 2 10,292,000 Typhoon FLA 7000 IP 1 8,900,000FLA Image Eraser BAS 1 28-9564-73 432,000Typhoon FLA 7000 Extended Warranty 2 960,000EcoRepro 41 146 32 Typhoon FLA 7000 Extended Warranty 2 34 960,000190 Typhoon FLA 7000 Extended Warranty Plus 2 Typhoon FLA 7000 1 34 1,056,00034 800,000Extended Warranty Plus 96,000 2 640,000 132 www.gelifesciences.co.jp