QuickGene mini 80 - Lab Services BV

Personal nucleic acid isolation device, one forQuickGene-Mini80 is a compact system requiring no centrifugation in the extraction process, giving less strain to samples and enablingrapid nucleic acid extraction. DNA/RNA can be easily extracted from various samples including human/mouse/plants/cells/fungi andothers. The QuickGene-Mini80 shares a common extraction mechanism and kits with our semi-automated nucleic acid isolationsystem, QuickGene-810. QuickGene-Mini80 offers high performance comparable to QuickGene-810 at a reasonable price.Compact DesignThe small, lightweight QuickGene-Mini80 takes up minimalspace on the lab bench and is easy to carry.QuickGene-Mini80 can be operated on the lab bench so, withoutmoving around, the user can perform nucleic acid extraction.Revolutionary Porous MembraneThe QuickGene-Mini80 uses FUJIFILM'spatented porous membrane only 80µm thick.Porous membraneGlass fiber membraneBecause of the outstanding absorptive anddesorptive performances of the membrane,high-purity nucleic acid can be easily obtainedin high yield at low pressure.The ultra thin membrane enables nucleic acidextraction in shorter time than when comparedto glass fiber membranes.Cartridge50µm50µmHigh Purity, High YieldHighly reproducible QuickGene-Mini80 can stably extract nucleic acid in high yield.The extracted DNA/RNA contains few impurities and can be used directly in PCR and RT-PCR.Length of extracted DNAExtraction of plasmid DNAExtraction of DNA fromtissueExtraction of RNA fromtissueQuickGene DNA tissue kit S QuickGene Plasmid kit S QuickGene DNA tissue kit SQuickGene RNA tissue kit SM1M2 M3TailLiverMpBS /GAPDH/DH5 (1×10 9 )MTailMLiverLiver Spleen HeartDNase DNase DNaseM + - + - + -190-260145kb121kb97kb73kb48kb24kb23.1kb9.4kb6.6kb4.4kbM :1kb PLUS DNA Ladder M :1kbp PLUS DNA Ladder M :1kbp PLUS DNA LadderYield and purity of plasmid DNAYield and purity of genomic DNAYield and purity of total RNA2.0kbM1: Hind digestM2:MidRange PFG MarkerM3:Mouse Genomic DNA(BD)∗Balb/c mouse (female, 7week old)Average*DNA yield(µg) 23.0Purity(260/280) 1.97Purity(260/230) 2.28*Average of 4 samplesDNA yield(µg)Purity(260/280)Purity(260/230)Tail4.01.9162.117Liver3.61.9251.942∗Balb/c mouse (female, 7week old)∗Without RNase treatmentDNase Livertreatment 32mg+ 155.4total RNA yield(µg)- 159.3+ 2.1Purity(280/260)- 2.1+ 2.3Purity(280/230)- 2.2∗Balb/c mouse (female, 7week old)Spleen32mg71.8Heart32mg21.274.3 21.62.12.12.22.22.1 2.02.1 1.8

each personEasy & Rapid ProcessingThe operation is simple. Just set the sample and rotate the blue pressurizing Rotary Switch on both sides of the system.Centrifugation is not required for the extraction. The operation is simple, and saves valuable time.Easy and simple extraction operation1 2 3Apply lysateSet (tube/cartridge) holder∗Commercially available microtubes are also usable.Turn the Rotary Switch to pressurizeWorkflow of extraction using DNA whole blood kitPrepare lysateComplete lysateApply whole lysateto cartridgePressurizeDNA can be extracted from human whole blood in only 6 min.**Represents the actual time required to perform the operation accordingto our protocols.Extraction processPressurizePressurizePressurizegenomic DNAWash buffer 750µlWash buffer 750µlTransfer holderto Elution PositionElution buffer200µlpressurizingprocessSet holder into systemRotate pressurizing switch toward the front side to start pressurizingMake sure that there is no residual liquid in the cartridge and return the pressurizing switch to original positionPull out holder from systemExtraction of RNA fromcultured cellsExtraction of RNA from cellscultured in 6 cm dishExtraction of RNA fromblood cellsQuickGene RNA cultured cell kit S QuickGene RNA cultured cell HC kit S QuickGene RNA blood cell kit SLeukocyte(1×10 7 )M HL60(1×10 6 ) M HEK293 M DNase(+) M DNase(-)M :1kbp PLUS DNA LadderYield and purity of total RNAM :1kb PLUS DNA LadderYield and purity of total RNAM :1kb PLUS DNA LadderYield and purity of total RNAtotal RNA yield(µg)Purity(260/280)Purity(260/230)*Average of 4 samplesDNasetreatment+-+-+-Average*1211.12.142.122.172.25Cell typeCell formHL60PelletHEK293*total RNA yield(µg)Purity(260/280)45.61.99110.51.86HeLa COS-7 NIH/3T36cm dish46.9 43.02.19 2.0734.41.93Purity(260/230) 2.01 1.98 1.98 1.96 1.91*HEK293 is the average of 4 samples.Leukocyte(1×10 7 )total RNA yield(µg)Purity(260/280)Purity(260/230)*Average of 2 samplesDNasetreatment+-+-+-Average*4.75.92.302.101.290.68

Easy extraction from various samplesAppropriate kit selectable depending on sample.Extraction examplePurposeCorresponding kitsWhole blood,buffy coatHuman/ Cow, etc.: Whole blood(EDTA blood, heparin blood)genomic DNA extraction for genetic test,genotypingDNA wholeblood kitTissueMaterials,manufactured goodsMouse/ Rat: Tail, Sperm, LiverWheat/ Red bean/ Pleurotaceae/ Shimeji mushroom, etc.DNA extraction for genome analysis ofsamples ranging from mouse/ rat to foodDNA tissue kitPlasmidE. coliMiniprep for plasmidPlasmid kitTissueMouse/ Rat: Liver, Kidney, Brain, Spleen, Lung,Heart, Thymustotal RNA extraction for expression analysissuch as real-time PCR and RT-PCRRNA tissue kit IICultured cellTissueAdherent/ Non-adherent (HeLa, HL60, etc.)Dicotyledonous plant (leaf)/ Tobacco (leaf), Plantstotal RNA extraction for expression analysissuch as real-time PCR and RT-PCRRNA culturedcell kitCultured cellCultured cell on 6cm/ 10cm dishHigh-yield total RNA extraction fornorthern blotting and microarrayRNA culturedcell HC kitBlood cellLeukocytes separated by ammonium chlorideErythrocytes of whole blood or Ficoll fractionHigh-purity total RNA extraction forexpression profiling or various applicationsRNA bloodcell kit*This chart shows extraction examples. Protocol may vary depending on samples and sample conditions.The specific set of isolation kits supports various samplesQuickGene Isolation kits are optimized for the QuickGene system to extract DNA and RNA in the shortest time and with the highest quality.Appropriate kit selectable depending on sample.Isolation kitsExtraction exampleQuickGene DNA whole blood kit Sfor 96 samplesReference code DB-Sca.5µg / Whole blood 200µlQuickGene DNA tissue kit Sfor 96 samplesReference code DT-Sca.4µg / 5mg Balb/c Mouse tailQuickGene Plasmid kit Sfor 96 samplesReference code PL-Sca.2.5µg / 1ml culture DH5QuickGene RNA tissue kit Sfor 96 samplesReference code RT-S2ca.100µg / 30mg Mouse liverQuickGene RNA cultured cell kit Sfor 96 samplesReference code RC-Sca.10µg / 1´10 6 cell HL60 cellQuickGene RNA cultured cell HC kit Sfor 96 samplesReference code RC-S290~150µg / 10cm dish cultured HEK293 cellQuickGene RNA blood cell kit Sfor 96 samplesReference code RB-Sca.4.5µg / 1´10 7 cell Leukocytes*The isolation kits used in QuickGene-Mini80 are also used for QuickGene-810.SpecificationsOverviewThroughput: 1 to 8 samples per runOperating conditionsPhysical specificationsDimensions : 280(W)´220(D)´180(H)mmWeight : Approx. 3 kg280220unit:mmSupply voltagePower supply frequencyOperating conditions: AC~100~240V: 50 / 60Hz: Temperature: 15~30°CHumidity: 30~80%(non-condensing)180Application Guides Available on our website.http://lifescience.fujifilm.comFUJIFILM Corporation 26-30, Nishiazabu 2-Chome, Minato-ku, Tokyo 106-8620, Japan, Tel: +81-3-3406-2201, Fax : +81-3-3406-2158, E-mail: sginfo@fujifilm.co.jpFUJIFILM Europe GmbH Heesenstr. 31, 40549 Dusseldorf, Germany, Tel: +49-211-5089-174, Fax : +49-211-5089-9144, E-mail: lifescience@fujifilmeurope.deFUJIFILM UK Ltd. Unit 12 St Martins Way, St Martins Business Centre, Bedford, MK42 OLF, U.K, Tel: +44-1234-245291, Fax : +44-1234-245293, E-mail: lifesciences@fuji.co.uk31st floor, Hong Kong New World Tower, No.300 Huai Hai Zhong Road, Shanghai, P.R China, Tel: +86-21-3302-4655 ext.363, Fax : +86-21-6384-3322, E-mail: wgxiang@fujifilm.com.cnhttp:// lifescience.fujifilm.comSpecifications and system configuration subject to change for improvement without notice. All other product names mentioned herein are the trademarks of their respective owners.