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Biofilm Hypothesis of Chronic Infection Teleclass ... - Webber Training

Biofilm Hypothesis of Chronic Infection Teleclass ... - Webber Training

The

The Biofilm Hypothesis of Chronic InfectionProf. PhilipA Webber Training TeleclassTreatment with 50 mg/l nisinDaptomycin0 min 10 min 20 min 30 min 40 minDavison et al (2010) Antimicrob. Agents Chemother. 54:2920.BodipyFL-Daptomycin penetration in biofilmStewart et al (2009)Antimicrob. AgentsChemother.53:3505.H 2 O 2 fails to penetrate biofilmAntimicrobial penetrationHydrogen Peroxide Concentration (mM)Bulk fluidcatalaseH 2 O 2 H2 O + 1/2O 2Base of biofilmTime (seconds)Stewart et al (2000)Appl. Environ.Microbiol. 66:836.Antibiotics, peptides penetrate readilyReactive oxidants may fail to penetrateHosted by Martin Kiernan, martin@webbertraining.comwww.webbertraining.com4

The Biofilm Hypothesis of Chronic InfectionProf. PhilipA Webber Training TeleclassColony biofilm3-day old S. aureus colony biofilmMembraneColonyAgarS. epidermidis killing by rifampinS. epidermidis colony biofilm-10biofilmLog Reduction12345planktonicZheng and Stewart(2002) Antimicrob.Agents Chemother.46:900.60 10 20 30 40 50Time (h)ControlRifampinDNA synthetic activity in biofilmOxygen profile in colony biofilmairnutrients BrdUDNA synthesisBiomassRani et al(2007) J.Bacteriol.189:4223.Oxygen Concentration (mg/l)7654321air biofilmRani et al (2007)0-50 0 50 100 150 200 250Depth ( µ m)J. Bacteriol.189:4223.Hosted by Martin Kiernan, martin@webbertraining.comwww.webbertraining.com5

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