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Biofilm Hypothesis of Chronic Infection Teleclass ... - Webber Training

Biofilm Hypothesis of Chronic Infection Teleclass ... - Webber Training

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The Biofilm Hypothesis of Chronic InfectionProf. PhilipA Webber Training TeleclassPhysiological heterogeneityK. pneumoniae biofilm - growthGrowing aerobicallyGrowing fermentativelyDeadDormant100 µmProtein synthetic activity in biofilmPseudomonas aeruginosa biofilmsConsensus genes - 10 studiesTranscriptomicWhiteley 2001Hentzer 2005Waite 2006Mikkelsen 2009Folsom 2010ProteomicSauer 2002Seyer 2005Southey-Pillig 2005Mikkelsen 2007Patrauchan 2007Top 26 genes inPseudomonas aeruginosa biofilmsPA0139PA0263PA0515PA0588PA0713PA1555PA1556PA1673PA1746PA1904PA1905PA2274PA2386PA2782PA3126PA3309PA3572PA4067PA4211PA4217PA4236PA4352PA4610PA5427PA5460PA5475Table 3. Activities or functions in P. aeruginosa biofilms as indicated by overlap with independently derived gene lists.Pa biofilm consensus genesActivityP valueOxygen downshift ~0Oxygen limitation 4x10 -11Stationary phase 8x10 -8Phenazine biosynthesis 2x10 -6Peroxide stress 0.02------------------------------ ---------Iron limitation 0.25HSL quorum sensing 0.29Mg limitation 0.76Osmotic stress 0.77Efflux pumps 0.83c-di-GMP 0.84Nitrosative stress 0.88Hosted by Martin Kiernan, martin@webbertraining.comwww.webbertraining.com6

Reporter Activity (Miller Units)The Biofilm Hypothesis of Chronic InfectionProf. PhilipA Webber Training TeleclassTable 3. Activities or functions in P. aeruginosa biofilms as indicated by overlap with independently derived gene lists.Adaptive stress responseTable 3. Activities or functions in P. aeruginosa biofilms as indicated by overlap with independently derived gene lists.Catalase induction in Pa biofilm65Antimicrobialexposure6055biofilm + H 2 O 2katB-lacZ50Unstressed,susceptibleStress responseinduced, protected454035planktonic + H 2 O 230250 10 20 30 40 50 60Time (min)Elkins et al(2000) Appl.Environ.Microbiol.65:4594SummaryCenter for Biofilm EngineeringBiofilms can cause chronic infectionsBacteria in biofilms evade killing by hostantimicrobials and antibioticsMultiple mechanisms of protection in biofilmsA NationalScienceFoundationEngineeringResearchCenterestablishedin 1990www.biofilm.montana.edu01 March Developing a Sustainable and Effective Approach to Hygieneand Infection Prevention in Home and Everyday Life SettingsSpeaker: Dr. Sally Bloomfield, International Scientific Forum on Home Hygiene07 March (FREE … WHO Teleclass - Europe) Achievements in ImprovingInjection Safety WorldwideSpeaker: Prof. Chuck Gerba, University of ArizonaSponsor: World Health Organization First Global Patient Safety Challenge22 March Hand Hygiene: New Frontiers in Messaging and MeasurementSpeaker: Dr. Katherine Ellingson, Centers for Disease ControlSponsor: Diversey Inc.29 March Water and Infection ControlSpeaker: Andrew Streifel, University of MinnesotaPainting by Annette Foster2695_99csHosted by Martin Kiernan, martin@webbertraining.comwww.webbertraining.com7

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