RBL cell lines and Serum IgE as Alternatives to In Vivo Methods
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Expression human FcERI diminishes during cultureRBL hEIa-2B12 Time in culture2 weeks4 weeksBlanc6 weeks18HESI workshop Nice, 25 October, 2007
Critical variables determining RBL responses2. Membrane-bound allergen-specific IgE• Fraction allergen-specific IgE in total serum IgE• Role avidity (polyclonal response)3. Intrinsic cellular sensitivity of RBL Amount IgE necessary for 50% degranulationVariable during culture19HESI workshop Nice, 25 October, 2007
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