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RBL cell lines and Serum IgE as Alternatives to In Vivo Methods

RBL cell lines and Serum IgE as Alternatives to In Vivo Methods

Critical variables

Critical variables determining RBL responses4. Cellular reactivity• Maximum response after optimal IgE-mediated stimulationVariable during culture• Positive controle: which one?20HESI workshop Nice, 25 October, 2007

Positive control. What is 100% release???Assay: Serum IgE + allergenI. Triton-X max. release available β-hex in assayII.Purified human IgE + anti-IgE max. release under optimal IgE-mediated stimulationIII.Serum IgE + anti-IgE max. release induced by serum more ‘physiological’ circumstances21HESI workshop Nice, 25 October, 2007

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