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RBL cell lines and Serum IgE as Alternatives to In Vivo Methods

RBL cell lines and Serum IgE as Alternatives to In Vivo Methods

Variables determining

Variables determining RBL responses6. Allergen structureo number of Ig binding epitopes better crosslinking?o aggregates allergeno purified allergen identical to ‘in vivo’ allergen etc.o Antigen RAST/CAP never identical to usage in RBL assay7. Choice serum• Clinically well documented sera (DBPCFC)• Which antigens / epitopes recognized?• Avidity IgE to antigen• Influence concentrated serum to viability RBL (>20%)• Optimization each assay per serum26HESI workshop Nice, 25 October, 2007

Conclusions• RBL assay relatively unsensitive, not to be used for diagnostics• Opportunities for screening allergenicity novel foods:‣Use well characterized antigens (number epitopes,aggregates, monomers etc)‣Use well characterized sera (which antigens / epitopes,preferrably DBPCFC); Unpooled / no loss viability‣Optimized culturing strategy27HESI workshop Nice, 25 October, 2007

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