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RBL cell lines and Serum IgE as Alternatives to In Vivo Methods

RBL cell lines and Serum IgE as Alternatives to In Vivo Methods

Outline•

Outline• Introduction Rat Basophil Leukemia cells (RBL)• RBL assay• Evaluation of three RBL cell lines• Critical variables RBL responses• Conclusions2HESI workshop Nice, 25 October, 2007

IntroductionType I hypersensitivity reactions• Critical factor: allergen-specific IgEHuman:• In vitro:- human basophils- RBL assayAdapted fromImmunobiology,Janeway3HESI workshop Nice, 25 October, 2007

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