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TM10-S2-2 Marianne Stef.pdf

TM10-S2-2 Marianne Stef.pdf

DETECTION OF COPY NUMBER

DETECTION OF COPY NUMBER CHANGES• Specific controls included in the chip and in each PCR group:• Normalization : Chromosome 21• Copy number change detection : Chromosome X• In each batch of hybridization, male and female controls areprocessed• Based on ratio of intensities of hybridizationChr 21Chr XNormalexonDeletedexonIsample (male)1000 500 1000 500Icontrol (female) 1000 1000 1000 1000Ratio 1 0.5 1 0.5

DETECTION OF COPY NUMBER CHANGES

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