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Chapter 6

Chapter 6

When synthesis of the

When synthesis of the first protein terminates, the ribosomes leavethe mRNA and dissociate into subunits.A ribosome physically spans ~30 bases of mRNA, so when it couldsimultaneously contact a termination codon and the next initiationsite if they are separated by only a few bases, the ribosomes do notassociate and start synthesizing the next peptide.

Virtually all eukaryotic mRNAs are monocistronic, but each mRNA usuallyis substantially longer than necessary just to code for its protein. Theaverage mRNA in eukaryotic cytoplasm is 1000-2000 bases long, has amethylated cap at the 5′ terminus, and carries 100-200 bases of poly(A) atthe 3′ terminus.The nontranslated 5′ leader is relatively short, usually

Cells: The Living Units: Part D -
Chapter 6, Ribosome Display, In Vitro Expression Guide ... - Promega
handouts for Chapter 6 - KVI
Chapter 6 Cutaneous Glands