Plasmid

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Plasmid

2010/11/21Identifying bacteria that have taken upgenetically altered plasmids (3)13Gene forresistanceto antibioticPlate withantibioticcontainingmediumBacteria with plasmidlive and multiply4Bacteria withoutplasmid fail to grow3 3 Because therecombinant plasmidsretain a gene forresistance to anantibiotic (R), bacterialcells that contain theplasmids are resistantto that antibiotic.Fig. 15-5, p. 298Identifying bacteria that have taken upgenetically altered plasmids (4)14Gene forresistanceto antibioticPlate withantibioticcontainingmediumBacteria with plasmidlive and multiply44Bacteria withoutplasmid fail to grow4The bacteria are thengrown on an antibioticcontainingnutrientmedium, and only thosethat contain therecombinant plasmidsurvive.Fig. 15-5, p. 2987

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