2-Dimensional GCMS with Cryotrap: Theory and Applications ...

2D-GC without Cryotrap:Oven temperature ramps superimposed with the cut window and thechromatogramThe oven ramp begins for column-2 when the oven is already close to its maximum.Oven maxOven TemperatureRamp for column-1Ramp for column-2Cut windowColumn-1 ChromatogramTemperatureTime2D-GC with Cryotrap: The cryotrap freezes analytes at the beginning ofcolumn-2.Peak-of-interestInjectionPort27 psiFIDCut window. Trapped in theCryotrap until thermally released.MSDColumn-1 Column-2GCOven20 psiCryotrapDeans SwitchAn improvement2D-GC WITH CRYOTRAP:• In practice, the cut to column-2 occurs when the oven is already at a hightemperature.• The high starting temperature for column-2 reduces its chromatographicresolution potential.• The introduction of a cryotrap allows the oven ramp for column-2 to beginat a lower temperature.Oven and cryotrap temperature ramps superimposed with the cutwindow and the chromatogram1. During the oven ramp for column-1, the cryotrap cools in preparation for the cut-window.2. The analytes are diverted to from column-1 to column-2 by the deans-switch where theyare trapped by the cold cryotrap.3. The oven is cooled.4. The cryotrap is rapidly heated to release the analytes.5. The second oven ramp begins for column-2.Cryotrap TemperatureCut windowRamp for column-2Oven TemperatureRamp for column-1ChromatogramTemperatureTime