Cosmosil Protein-R columns - Hplc.eu
Cosmosil Protein-R columns - Hplc.eu
Cosmosil Protein-R columns - Hplc.eu
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Wide Pore HPLC Column for <strong>Protein</strong> ChemistryCOSMOSIL <strong>Protein</strong>-R・Excellent Separation・High recovery rate・Outstanding Stability at Low pHSilica GelAverage Particle SizeAverage Pore SizeStationary PhaseMain InteractionHigh Purity Porous Spherical Silica5 μmapprox. 300ÅOctadecyl GroupHydrophobic InteractionSiOHOctadecyl GroupCOSMOSIL <strong>Protein</strong>-R is a reversed phase HPLC column designed specificallyfor protein and peptide separation. COSMOSIL <strong>Protein</strong>-R provides significantlyimproved peak shapes, high recovery rate and outstanding stabilityat low pH, which are often problematic for the separation of proteins andpeptides with conventional C18-300Å and C4-300Å <strong>columns</strong>.Improved SeparationCOSMOSIL <strong>Protein</strong>-R shows sharper peaks than conventional C18 wide pore <strong>columns</strong>.COSMOSIL <strong>Protein</strong>-RC18-300(A company)C4-300(B company)Column SizeMobile PhaseFlow RateTemperatureDetection4.6 mm I.D. x 150 mmA: 0.05% TFA - 20% AcetonitrileB: 0.05% TFA - 60% AcetonitrileB conc. 0 100% 20 min Linear Gradient1.0 ml/min30UV 220nmSample1. Ribonuclease A2. Cytochrome C3. Lysozyme4. BSA5. Myoglobin6. Ovalbumin(1.0 μg each)(MW: 13700)(MW: 12400)(MW: 14300)(MW: 66000)(MW: 17000)(MW: 45000)Recovery RateCOSMOSIL <strong>Protein</strong>-R shows a higher recovery rate thanconventional C18-300Å and C4-300Å <strong>columns</strong>.DurabilityCOSMOSIL <strong>Protein</strong>-R provides more consistent retentiontime and longer column lifetimes than conventional C4-300Å .<strong>Protein</strong>-RC18-300(A company)C4-300(B company)Myoglobin (M.W. 17000)BSA(M.W. 66000)Cytochrome C (M.W. 12400)Ribonuclease A (M.W. 13700)120100 80604020 COSMOSIL <strong>Protein</strong>-R C4-300(B company)0% 20% 40% 60% 80% 100%0050 100time (hr)C4-300ÅC18-300ÅProblemsWeak Acid ResistanceLow Separation CapacityLow Recovery RateAdvantages of COSMOSIL <strong>Protein</strong>-RCOSMOSIL <strong>Protein</strong>-R solves these problems, and providesdramatic improvement of separation capasity and recoveryrate with high acid resistance.
Preparative Separation4.6 mm I.D. x 250 mm 4.6 mm I.D. x 250 mm10.0 mm I.D. x 250 mmTotal <strong>Protein</strong> 6 μg (1 μg each) Total <strong>Protein</strong> 600 μg (100 μg each) Total <strong>Protein</strong> 3000 μg (500 μg each)Sample1. Ribonuclease A (MW: 13700)2. Cytochrome C (MW: 12400)3. Lysozyme (MW: 14300)4. BSA5. Myoglobin6. Ovalbumin(MW: 66000)(MW: 17000)(MW: 45000)Application Data・Peptides・High Molecular Weight <strong>Protein</strong>sColumn SizeMobile PhaseFlow RateTemperatureDetectionSample4.6 mm I.D. x 150 mmA: 0.05% TFA / WaterB: 0.05% TFA / AcetonitrileB conc. 10 40% 20 min Linear Gradient1.0 ml/min30 o CUV 220 nm1. Oxytocin2. Angiotensin II (Human)3. Angiotensin I (Human)4. Substance P(0.8 μg each)Column SizeMobile PhaseFlow RateTemperatureDetectionSample4.6 mm I.D. x 150 mmA: 0.05% TFA / 20%AcetonitrileB: 0.05% TFA / 60%AcetonitrileB conc. 0 100% 10 min Linear Gradient1.0 ml/min30 o CUV 220 nm, 0.256AUFS1. Fibrinogen (4.0 μg)2. Catalase (2.0 μg)3. Thyroglobulin (6.0 μg)・Peptides5C18-MS-II (pore size 120) shows better separation of low molecular weight proteins, but COSMOSIL <strong>Protein</strong>-R shows better separation of high molecular weight proteins.Column SizeMobile PhaseFlow RateTemperatureDetectionSample4.6 mm I.D. x 150 mmA: 0.05% TFA / WaterB: 0.05% TFA / AcetonitrileB conc. 0 35% 60 min Linear Gradient1.0 ml/min30 o CUV 220 nmPoly-L-Glutamic Acid Sodium Salt(MW: 1500-5000) (200 μg)5C18-MS-II<strong>Protein</strong>-ROrdering InformationProduct Name Column Size Product NumberCOSMOSIL <strong>Protein</strong>-R 2.0 mm I.D. x 150 mm 06514-71Packed Column 4.6 mm I.D. x 50 mm 06525-314.6 mm I.D. x 150 mm 06526-214.6 mm I.D. x 250 mm 06527-1110.0 mm I.D. x 150 mm 06529-9110.0 mm I.D. x 250 mm 06530-5120.0 mm I.D. x 150 mm 06531-4120.0 mm I.D. x 250 mm 06532-31Other size may be available. Please enquire.Product Name Column Size Product NumberCOSMOSIL <strong>Protein</strong>-R 4.6 mm I.D. x 10 mm 06518-31Guard Column 10.0 mm I.D. x 20 mm 06528-0120.0 mm I.D. x 20 mm 08692-81
COSMOSIL C18-AR-300, C8-AR-300, C4-AR-300, Ph-AR-300COSMOSIL offers a variety of stationary phases with wide-pore silica gel material for separations of polypeptides and proteins.Stationary Phase5C18-AR-300 5C8-AR-300 5C4-AR-300 5Ph-AR-300SiOHSiOHSiOHSiOHOctadecyl Group Octyl Group Butyl Group Phenyl GroupCarbon Content approx. 12% approx. 7% approx. 6% approx. 7%USP Category L1 L7 L26 L11Main InteractionHydrophobic InteractionHydrophobic Interactionπ-π InteractionPacking MaterialHigh Purity Porous Spherical SilicaAverage Particle Size5 μmAverage Pore Sizeapprox. 300 ÅComparison of Separation5C18-AR-3005C8-AR-300 5C4-AR-300 5Ph-AR-300Column SizeMobile PhaseFlow RateTemperatureDetection4.6 mm I.D. x 150 mmA; 0.05%TFA - 20%AcetonitrileB; 0.05%TFA - 60%AcetonitrileB conc. 0 100% 20min Linear Gradient1.0 ml/min30UV 220 nmSampleHemoglobin, Bovine (10 μg)For more data on C18-AR-300, C8-AR-300, C4-AR-300, Ph-AR-300, please visit our website at www.nacalai.comCOSMOSIL ApplicationCOSMOSIL Application, which includes more than 7,000 applications using COSMOSIL <strong>columns</strong>, is available on our webiste.The application is searchable by sample category, sample name, CAS No., column name and particle size. For more information,please visit www.nacalai.com
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