Quick Protocols

PetakaG3 TMRecovering cellsfrom “dormancy”RECOVERING CELLSFROM “DORMANCY”1. Disinfect port with 90% Ethanol & aflame2. Slowly withdraw the medium3. Inject the same volume of NEWmedium with the same additives4. Whilst holding the Petaka in a verticalposition, squeeze it until the level of media reaches the air exit & thenrelease until the air input stops.Then the inside/outside pressure willbe balanced.5. Incubate at 37ºC, in a vertical position,at least for 24 hours before cellSUBCULTUREPetakaG3 TMpH adjustment ofgrowing culturespH ADJUSTMENT WITHSODIUM BICARBONATE1. Disinfect port with 90% Ethanol & aflame2. Withdraw 1ml of Medium3. Whilst holding the Petaka in a verticalposition, squeeze it until the levelof media reaches the air exit & thenrelease until the air input stopsThen the inside/outside pressure will bebalanced4. Inject 1ml of 7.5% Sodium Bicarbonatesterile solution5. Shake gently for 10-15 seconds.6. Return the Petakas to the incubatorPetakaG3 TMMedia changeMEDIA CHANGE1. Disinfect port with 90% Ethanol & aflame2. Slowly withdraw all the mediumcompletely3. Inject the same volume of NEWmedium with the same additives4. Whilst holding the Petaka in a verticalposition, squeeze it until the levelof media reaches the air exit & thenrelease until the air input stops.Then the inside/outside pressure willbe balanced.5. Incubate at 37ºC, in a vertical position,at least for 24 hours before cellSUBCULTURE6. Return Petaka to the incubator