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SPECTROPHOTOMETRYIntroductionUV/visible spectrophotometers are a ubiquitoustool in the life sciences because of the wide rangeof molecules that can be detected and analysedusing this technique. Like many other everydayitems, it’s easy to forget how long you have hadyour spectrophotometer and you might not thinkabout its performance or capabilities until you needto replace it. Questions to consider include:• What applications are routinely run in yourlaboratory? Could this change in the near future?• What range of sample volumes do you need tomeasure? If this varies, look for options for differentcuvette sizes and adaptors• Do you want to measure multiple samples atthe same time? If so, consider options for multiplecell holders• What level of sensitivity do you need? For lowconcentration samples, a wider bandwidth(3 to 5 nm) will give more consistent resultsthan a narrow (1 nm) bandwidth instrument• How many users will there be and what levelof experience will they have? Pre-programmedmethods and password control of your ownmethods can be useful features• How will data be recorded, output or stored?This guide is an introduction to the range ofspectrophotometers available from GE HealthcareLife Sciences, including new instruments for lowsample volumes and new features such as theSD card option.Whether you are performing simple colorimetricassays or conducting the most stringent research,there is an instrument from our range to meetyour needs.Low sample volumes and ease of useNanoVue Plus Spectrophotometer offers convenienceand speed using a novel drop-and measure sampleplate for the accurate and reliable quantitationof nucleic acid and protein samples. The ability topipette samples of 0.5 to 5 μl directly onto the sampleplate eliminates the need for cuvettes or othersample devices.Routine applicationsGE Healthcare offers a number of spectrophotometersfor widely used applications such as DNA and RNAquantitation, protein concentration determination,and cell density measurements. This includesUltrospec 10, a battery-operated, portable,cell density meter; Novaspec III and Plus forprotein determination and/or enzyme kinetics;and GeneQuant 100 and 1300 instruments.Additional featuresFor more advanced features, Ultrospec 2100 offerstemperature control and cell changer options, whilstthe Ultrospec 8000 and 9000 are high performance,dual beam instruments, for precision measurementsthat are compatible with Pharmacopoeia guidelines.2 | GLOBAL SCIENCE A VWR COMPANY | 0800 734 100 | SALES@GLOBALSCIENCE.CO.NZ | WWW.GLOBALSCIENCE.CO.NZ |
Spectrophotometer selection guideModelMain featuresDNA/RNA quantitationPCR/sequencing/hybridizationPrimer quantitation and designProtein determination(Bradford, Biuret, BCA etc)Cell culture OD600Enzyme activity kinetics at 340 nmFluorescent probe quantitation, cDNAprobes for microarrays, PCR probesPharma method developmentUltrospec 9000High-performance dual-beam, variable bandwidthUV-Visible spectrophotometer. Wavelength range190-1100 nm. European Pharmacopeia compatible.Optional Life Science Datrys software upgrade available.Ultrospec 8000Ultrospec 7000High-performance dual-beam, 1 nm bandwidth UV-Visiblespectrophotometer. Wavelength range 190-1100 nm.European Pharmacopeia compatible. Optional Life ScienceDatrys software upgrade available.High-performance dual-beam, 2 nm bandwidthUV-Visible spectrophotometer. Wavelength range190-1100 nm. Optional Life Science Datrys softwareupgrade available.Ultrospec 2100 pro 3 nm bandwidth instrument, wavelength range 190to 900 nm with 8-position cell changer and options fortemperature controlNanoVue PlusGeneQuant 1300GeneQuant 100Novaspec IIINovel sample mechanism, eliminates need for cuvettesand facilitates low-volume measurements (0.5 to 5 μl)As GeneQuant 100, with built-in enzyme kinetics, andCyDye applications plus Bluetooth data output options5 nm bandwidth instrument with built in applicationsfor nucleic acids, proteins and cell density7 nm bandwidth visible spectrophotometer, 330 to 800 nmNovaspec PlusUltrospec 10As Novaspec III, with stored protein methods, easy toread graphical display, and temperature control optionBattery-powered, portable cell density meterKey: Recommended Suitable Not suitable| ALL PRICES EXCLUDE GST & FREIGHT | PRICES VALID UNTIL 30 JUNE 2012 | GLOBAL SCIENCE A VWR COMPANY |3
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Page 3: ContentsIntroduction 2Spectrophotom
Page 7 and 8: Ultrospec 8000/8000PC UV-Visible sp
Page 9 and 10: Ultrospec 7000, 8000, and 9000 acce
Page 11 and 12: Ultrospec 2100 proOptions forenzyme
Page 13 and 14: GeneQuant 1300Compact, convenientan
Page 15 and 16: Ultrospec 10 Cell Density MeterHand
Page 17 and 18: Datrys System control softwareSoftw
Page 19 and 20: Related productsAccessories for Ult

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