IARA Vol. XXVI No. 1 Year. 2009 (pdf, 1420 kb) - Pustaka Deptan

Vol. 26, No. 1, 2009Indonesian Agricultural Research AbstractsIndonesia, Jember (Indonesia)). Pelita Perkebunan ISSN 0215-0212 (2005) v. 21(2) p. 73-89, 4 ill., 5tables; 26 ref.COFFEA CANEPHORA; CHITINASE; GENETIC ENGINEERING; TRANSGENIC PLANTS;GENETIC TRANSFORMATION; SOMATIC EMBRYOS; GENETIC MARKERS.Genetic engineering of robusta coffee resistant to fungal diseases might be done by introducing achitinase-encoding gene into genome of this plant. This research was aimed to confirm transgenic plant ofBP 308 clone robusta coffee transformed by chi gene and to evaluate its ability for the somaticembryogenesis. Confirmation of transgenic was carried out by analyzing the presence of NPTII gene as aselectable marker for Canamysin resistant using PCR technique. The somatic embryo initiation andreproduction were evaluated in 11 plant accessions. Three kinds of sucrose concentration (20%, 30% and40%) were applied in initiation stage of somatic embryo germination. The suitability of 4 medium, namelyM1 (without addition by liquid medium), M2 (addition by liquid medium contained 0.25 mg/l kinetin),M3 (addition by liquid medium contained 0.25 mg/l IAA), and M4 (addition by liquid medium contained0.25 mg/l GA3) was evaluated for somatic embryo maturation. The result showed that 8 out of 10 plantaccessions tested were transgenic and they could be propagated through somatic embryogenesis. Theability of transgenic plant for somatic embryo initiation, reproduction and regeneration were similar withthat of non-transgenic one. Germination of somatic embryo could be improved by using 40% sucrose.Maturation of somatic embryo could be improved by addition of fresh liquid medium on the ancient gelledmedium that used for somatic embryos reproduction. The best result was obtained on addition of freshmedium contained 0.25 mg/l GA3 in which 65% of the somatic embryos developed to pregerminatesomatic embryo.042 REFLINUR.Reaction of rice monogenic lines carrying blast disease resistance genes to Pyricularia grisea isolatein Indonesia. Reaksi galur padi monogenik pembawa gen ketahanan penyakit blas dari beberapa isolatPyricularia grisea di Indonesia/Reflinur; Bustamam, M. (Balai Besar Penelitian dan PengembanganBioteknologi dan Sumberdaya Genetik Pertanian, Bogor (Indonesia)); Widyastuti, U.; Aswidinnoor, H.Penelitian Pertanian Tanaman Pangan ISSN 0216-9959 (2006) v. 25(1) p. 9-14, 1 ill., 4 tables; 14 ref.ORYZA SATIVA; UPLAND RICE; VARIETIES; MAGNAPORTHE GRISEA; GENETICRESISTANCE; BLIGHT; GENES; INDONESIA.Rice blast disease caused by Pyricularia grisea is one of the most important diseases of upland rice inIndonesia. The use of resistant varieties is an effective way to control rice blast disease. A set of resistancemonogenic lines is now available, but its effectively was still unknown in Indonesia. To investigate theeffectiveness of rice blast resistance gene carried by monogenic lines, phenotypic tests by usingIndonesian blast isolates were required. The effectiveness of the 31 monogenic lines were tested acrossnine rice blast isolates. The young leaves of 21 day old plants were sprayed with 50 ml of inoculumsuspension (2 x 10 6 conidia/ml). The symptom of disease as observed at seven days after inoculation wasdetermined. Score 0-3 was categorized as resistant, 4-6 as moderately resistant, and 7-9 was susceptiblereaction. The phenotypic analysis indicated that seven major resistant genes (Pik, Pik-h, Piz, Piz5, Pi1,Pi7(t) and Pik-m) were incompatible to nine tested isolates. The other three resistant genes which werealso effective namely Pik-p, Pish, and Pi9 were just compatible to one isolate but incompatible to the otherisolates. Three resistant genes, Piz-t, Pit, and Pi19 showed specific interaction to one of the tested isolates.The specific interaction was an incompatible reaction between Piz-t gene and R-173Skb isolate butcompatible to the others, while both Pit and Pi19 genes were incompatible to 04-012 isolate andcompatible to the other isolates.20