IARA Vol. XXVI No. 1 Year. 2009 (pdf, 1420 kb) - Pustaka Deptan

Vol. 26, No. 1, 2009Indonesian Agricultural Research Abstracts045 SETIYO, I.E.Genetic diversity analysis of interpopulation of oil palm tenera based on RAPD markers. Analisiskeragaman genetik Elaeis guineensis interpopulasi tenera berdasarkan marka RAPD/Setiyo, I.E. (PusatPenelitian Kelapa Sawit, Medan (Indonesia)). Jurnal Penelitian Pertanian ISSN 0152-1197 (2006) v.25(1) p. 60-70, 6 ill., 3 tables; 14 ref. Appendix.ELAEIS GUINEENSIS; GENETIC VARIATION; GERMPLASM; RAPD; POPULATION GENETICS;RECOMBINATION; HETEROZYGOTES.Availability of germplasm and its genetic diversity information is very important for creating superiorplant cultivar. Germplasm introduction and recombination are usually undertaken by breeders in order toenrich genetic diversity. Indonesian Oil Palm Research Institute (IOPRI) has integrated technology ofDNA based molecular marker into breeding program. Beside, in optimalization of germplasm utilization,the use of DNA technology is important for early identification of plant traits. In this experiment, thediversity of 16 elite families of oil palm tenera were analyzed its diversity using RAPD markers with fourselected oligonucleotide primers (OPD-16, OPN-09, OPM-16 and OPR-11). Calculation of geneticsimilarity coefficient and construction of their dendogram were carried out by using computer programNTSYSpc version 2.02. All primers used to amplify genome DNA generated total 22 fragment bands withpolymorphism level as many as 15 bands (68.5%). Estimated heterozygosity of SP 540 population waslower than that of Binga population. In general, recombination was able to increase value ofheterozygosity. Dendogram showed that in 47% coefficient of genetic similarity tenera populations wereseparated into four groups. In this similarity level, SP 540 was selfed population (family 1391 and 1540)and SP 540*Bangun population (family 1394) were clustered in one big group in concurrence with Bingapopulation (particularly family 1664, 1667 and 1670) into group I. Two individual palms of Bingapopulation of other family (1669 and 1677) segregated to group II and IV, respectively. All individual ofSP 540*Marihat (family 1543) clustered into group II. Group III composed of only one individual palm assegregation of family 1547 (SP 540*Polonia).046 SOBIR.Identification of cDNA fragment tightly linked to NV and TM-2 loci in tomato. Identifikasi fragmentcDNA yang berpautan dengan lokus NV dan TM-2 pada tomat/Sobir (Institut Pertanian Bogor(Indonesia). Fakultas Pertanian); Motoyoshi, F. Zuriat ISSN 0853-0808 (2005) v. 16(2) p. 111-120, 3 ill.,2 tables; 24 ref.LYCOPERSICON ESCULENTUM; LOCI; DNA; GENETIC RESISTANCE; RNA; IDENTIFICATION;COMBINING ABILITY; HYBRIDIZATION.Tm-2 is a resistance gene in tomato against tomato mosaic virus (ToMV), located in heterochromaticregion of chromosome nine. Since map based cloning was difficult to perform for identify the gene on thatregion, differential display approach was applied by using two near-isogenic tomato lines (NILs), onewithout Tm-2 and the other with Tm-2 to identify cDNAs of the transcripts from the region surroundingthe Tm-2 locus. Among the 150 combinations of three anchor primers and fifty arbitrary primers, 10combinations generated cDNA polymorphic bands. Out of them, only one combination of CA6, exhibited,polymorphic band under southern blot analysis, subsequently a genetic experiment showed that the CA6locus tightly linked to the Tm-2 locus. The CA6 fragment also hybridized to genomic DNA fragmentsfrom a tomato line carrying Tm-2a, a line of L. peruvianum from which Tm-2a was originated, and atomato line carrying another Tm-2-like gene. A northern hybridization blotting result suggested that thegene corresponding to CA6 fragment was constitutively transcribed.22