Molecular Biology - eScience Labs

esciencelabs.com

Molecular Biology - eScience Labs

MolecularBiologyAPBIOLOGYLab6:MolecularBiologyConceptscovered restrictionendonucleasesFigure6.1RestrictionendonucleasescansnipDNAintwoways,leavingeitherbluntendsorstickyends.


MolecularBiologyElectrophoresisFigure6.2Fillingthewellsofanelectrophoresisgel recombinantDNAFigure6.3AfluorescentdyethatbindsDNA,canbeusedtovisualizebandsafterthegelhasbeenrun.


MolecularBiologyConjugationTransductiontransformationFigure6.4Scientistsareabletoengineerplasmidssotheycontainageneofinterestfromanotherorganism.Figure6.5Incorporationofaforeigngenecanleadtocellswithnewcharacteristics.


MolecularBiologyoriantibioticresistanceengineerplasmidsPre-Laboratory Questions Useadditionalpaperasnecessary


MolecularBiologyExperiment6.1:RestrictionEndonucleasesandElectrophoresisMaterialsFigure6.6Procedure Table6.1


MolecularBiology(Note:Enzyme2and3meansthatfirst,enzyme2 fragmentstheDNA,thenenzyme3cutsthefragmentsmadebyenzyme2). Questions


MolecularBiologyLab6.2:Electrophoresis MaterialsNote:Gelsmustbeanalyzedimmediatelyafterelectrophoresis.Colorbandswilldiffuseandbleedovertime.1. Note:Ifthe10Xsolutionhasprecipitated,placethebottleinahotwaterbathandswirluntilprecipitantdissolves.


MolecularBiology2. Note:Theagarosewillbecomeclearwhenmelted,about4045seconds. 5. Hint:Thegroovesarenoticeablefromaboveandfromtheside,about1/4inchfromthetopandbottomofthechamber.Ifyouprefer,itmaybehelpfultobeeyelevelwiththechamberwhenpouringtheagarose.Whenlookingstraighton,fromalateralview,youcanseetheheightofthegroovesandwheretostopwhenpouringtheagarose. Hint:Useapipettetipto“poke”thebubblesormovethemtotheside. Hint:Youmayhavetosoftlyjigglethecomboutofthegel. Note:Thedyeshavebeenmixedwithasucrosesolutiontoincreasetheirdensityandinsureyoursamplewillfallgentlyintothewell.However,itisstillimportanttorunthegelrelativelyquicklysothedyesdonotdiffuseoutofthewells.


MolecularBiology Figure6.7Connectingtheleadstotheminigelchamberandpowersupply16. Remembertousea cleanpipetteforeachsample!


MolecularBiology Remembertousea cleanpipetteforeachsample! Note:DONOTletthedyesmoveoffthegel! Table6.2ElectrophoresisofDyeSamplesWell Sample #Bands Migration(cm) Questions


MolecularBiology


1500WestHampdenAvenueSuite5HSheridan,CO801103037410674888ESLKITSwww.eScienceLabs.com

More magazines by this user
Similar magazines