1 CATHERINE H. FREUDENREICH Curriculum ... - Tufts University

Curriculum Vitae
CATHERINE H. FREUDENREICH
Education and Experience
1984-1988 B.A. in Biology, Rice University, Houston, TX.
1984-1988 Undergraduate Research Assistant with Dr. Robert K. Moyzis, Los Alamos National Laboratory
Life Sciences Division, Los Alamos, NM. Human genomics, RFLP mapping.
1989-1994 Graduate study, Duke University, Durham, NC., Thesis advisor: Dr. Kenneth N. Kreuzer
Ph.D. Thesis: "Inhibition of the T4 Type II Topoisomerase by Antitumor Agents:
Determinants of Cleavage Site Specificity and Location of the Inhibitor Binding Site."
1994-1995 Postdoctoral Fellowship with Dr. Kenneth N. Kreuzer, Duke University, Durham, NC.
Research on T4 topoisomerase II inhibition by antitumor agents
1995-1999 Postdoctoral Fellowship with Dr. Virginia A. Zakian, Dept. of Molecular Biology, Princeton
University, Princeton, NJ.
Research on stability and fragility of trinucleotide repeat sequences in yeast
1999-2006 Assistant Professor, Department of Biology, Tufts University, Medford, MA.
2001-2006 Assistant Professor, Genetics Program, Tufts Sackler School of Graduate Biomedical Sciences
2006-present Associate Professor, Department of Biology, Tufts University, Medford, MA
2006-present Associate Professor, Genetics Program, Tufts Sackler School of Graduate Biomedical Sciences
Awards
NIH R01 Grant Support, 2001-2012
NIH postdoctoral fellowship, 1995-1998
Richard O. Burns Award for outstanding graduate research, Microbiology Dept., Duke University, 1994
DAAD (German Academic Exchange) Scholarship, 1988-1989
Honors and Scientific Service
Recent invited conference talks (2010-2012):
June 2012, FASEB conference, Dynamic DNA Structures in Biology
May 2012, EMBO workshop, Recombination Mechanisms and Genome Instability
April 2012, ASBMB meeting, DNA Replication, Recombination and Repair Symposium
March 2012, Gordon Conference, DNA Damage, Mutation and Cancer
April 2011, Society for General Microbiology, Harrogate, UK; Mechanisms of DNA Repair Symposium
February 2011, Keystone Meeting, DNA Replication and Recombination
Dec. 2010, Asilomar Chromatin and Chromosomes Conference
Aug. 2010, Gordon Conference, Mutagenesis (Lionel Gellon, post-doctoral fellow)
July, 2010, FASEB Meeting, Biological Impact of Alternatively Structured DNA, Steamboat Springs, CO
Recent invited university talks (2010-2012):
March 2012, Department of Biochemistry, Vanderbilt University
April 2011, Department of Biology at University of Copenhagen
June 2010, Dartmouth University Biology Dept. (graduate student invited speaker)
March 2010, Emory University Dept. of Human Genetics
Reviewer for Granting agencies:
2010 Netherlands Organization for Scientific Research (NWO)
2008 NSF Eukaryotic Genetics, Panel Member
2007 NSF Eukaryotic Genetics, ad hoc
2005 European Science Foundation (ESF) Young Investigator Awards
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2004 NIH panel member of CDF-2 special study section on DNA repair/damage/replication
2004 Medical Research Council (MRC)
2002 The Wellcome Trust, Molecular and Cell Biology Panel
Professional Memberships
Member, Genetics Society of America
Member, American Society for Microbiology
Teaching (years taught, 1999-2012)
General Genetics (4), Experiments in Molecular Biology (10), Seminar in Molecular Biology and Genetics (5),
Topics in Molecular/Cellular Biology (8), Big Bang to Humankind (4)
Graduate and Post-Graduate Trainees (1999-2012)
Graduate (Ph.D) – 4 students
Postdoctoral – 3 fellows
UndergraduateTrainees
2-4 undergraduates per semester/summer. 38 total students 2000-2012
11 Senior Honors Theses sponsored
Publications
1. Overcoming natural replication barriers: Differential helicase equirements. Ranjith P. Anand, Kartik A.
Shah, Hengyao Niu, Patrick Sung, Sergei M. Mirkin and Catherine H. Freudenreich. (2011) Nucleic Acids
Research Oct 7, PMID: 21984413.
2. Rangapriya Sundararajan and Catherine.H. Freudenreich. Expanded CAG/CTG Repeat DNA induces a
checkpoint response that impacts cell proliferation in S. cerevisiae. (2011) PloS Genetics March 17; 7(3)
e1001339.
3. Nicole Cherng, Alexander A. Shishkin, Lucas I. Schlager, Ryan H. Tuck, Laura Sloan, Partha S. Sarkar,
Tetsuo Ashizawa, Catherine H. Freudenreich and Sergei M. Mirkin Expansions of the SCA10
pentanucleotide repeat in yeast. (2011) Proceedings of the National Academy of Sciences Feb
15;108(7):2843-8.
4. Lionel Gellon, David F. Razidlo, Olive Gleeson, Lauren Verra, Danae Schulz, Robert S. Lahue, and
Catherine H. Freudenreich New functions of Ctf18-RFC in preserving genome stability outside its role in
sister chromatid cohesion. (2011) PloS Genetics Feb 10 7 (2) e1001298.
5. Jiahui Yang and Catherine H. Freudenreich. (2010) The RTT109 histone acetyltransferase facilitates errorfree
replication to prevent CAG/CTG repeat contractions. DNA Repair, Apr 4; 9(4):414-20.
6. Sundararajan R., Gellon, L., Zunder, R. M., and Freudenreich, C. H. (2010). Double-Strand Break Repair
Pathways Protect against CAG/CTG Repeat Expansions, Contractions and Repeat-mediated
Chromosomal Fragility in Saccharomyces cerevisiae. Genetics Jan 2010,184(1):65-77.
7. Irina Voineagu, Catherine H. Freudenreich, Sergei M. Mirkin (2009) Checkpoint responses to unusual
structures formed by DNA repeats. Molecular Carcinogenesis Volume 48 Issue 4 (p 309-318). (Invited
review for special issue: Intrinsic Genomic Instability from Naturally Occurring DNA Structures, Edited by
Karen Vasquez, Philip Hanawalt).
8. Alix Kerrest, Ranjith P. Anand, Rangapriya Sundararajan, Rodrigo Bermejo, Giordano Liberi, Bernard
Dujon, Catherine H. Freudenreich and Guy-Franck Richard (2009) SRS2 and SGS1 Prevent Chromosomal
Breaks and Stabilize Triplet Repeats by Restraining Recombination. Nature Structural and Molecular
Biology 16 (Feb) 159-167.
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9. Zhang H. and Freudenreich, C.H. (2007) An AT-rich sequence in human common fragile site FRA16D
causes fork stalling and chromosome breakage in S. cerevisiae. Molecular Cell 27, 367-379. (profiled in
Science News Focus “DNAs Molecular Gymnastics”, June 9, 2006 312: 1467-68.)
10. Freudenreich, C. H. (2007) Chromosome Fragility: Molecular mechanisms and cellular consequences.
Frontiers in Bioscience Sep. 1;12:4911-24. (Invited review for special edition "DNA Structures, Genome
Instability and Human Disease")
11. Yang, J. and Freudenreich, C.H. (2007) Haploinsufficiency of yeast FEN1 causes instability of expanded
CAG/CTG tracts in a length-dependent manner. Gene, Volume 393, Issues 1-2, 15 May 2007, Pages 110-
115.
12. Freudenreich, C. H. (2005) Molecular Mechanisms of Chromosome Fragility. ChemTracks-Biochemistry
and Molecular Biology, Vol 18, No.3, 141-152 (Invited review for special theme issue “Unstable
Microsatellites in the Human Genome, Part 2”).
13. C. H. Freudenreich and Mayurika Lahiri (2004) Structure-Forming CAG/CTG repeat sequences are
sensitive to breakage in the absence of Mrc1 checkpoint function and S-phase checkpoint signaling:
Implications for trinucleotide repeat expansion diseases. Cell Cycle Vol 3 (11) 1370-1374.
14. Lahiri M., Gustafson T.L., Majors E.R., Freudenreich C.H. (2004) Expanded CAG repeats activate the
DNA damage checkpoint pathway. Mol Cell 15: 287-293.
15. Liu Y., Zhang H., Veeraraghavan J., Bambara R.A., Freudenreich C.H. (2004). Saccharomyces cerevisiae
Flap Endonuclease 1 Uses Flap Equilibration to Maintain Triplet Repeat Stability. Mol Cell Biol. 24: 4049-
4064.
16. Callahan JL, Andrews KJ, Zakian VA, Freudenreich CH. (2003) Mutations in yeast replication proteins that
increase CAG/CTG expansions also increase repeat fragility. Mol Cell Biol. 23:7849-60.
17. Lenzmeier BA, Freudenreich CH. (2003) Trinucleotide repeat instability: a hairpin curve at the crossroads
of replication, recombination, and repair. (Invited review) Cytogenet Genome Res. 100(1-4):7-24.
18. Balakumaran, B. S., Freudenreich, C. H., and Zakian, V. A. (2000) CGG/CCG repeats exhibit orientationdependent
instability and orientation-independent fragility in Saccharomyces cerevisiae. Human Molecular
Genetics 9: 93-100.
19. Freudenreich, C. H., Kantrow, S. M., and Zakian, V. A. (1998) Expansion and Length-Dependent Fragility
of CTG Repeats in Yeast. Science 279: 853-856.
20. Freudenreich, C. H., Stavenhagen, J. B. and Zakian, V. A. (1997) Stability of a CTG/CAG trinucleotide
repeat in yeast is dependent on its orientation in the genome. Molecular and Cellular Biology, 17: 2090-
2098.
21. Kreuzer, K. N., Freudenreich, C. H., and Pommier, Y. (2001) Analysis of cleavage complexes using
reactive inhibitor derivitives. Methods in Molecular Biology: Protocols in DNA Topology and DNA
Topoisomerases, 95:89-99. (Humana Press).
22. Freudenreich, C. H., Chang, C., and Kreuzer, K. N. (1998) Mutations of the bacteriophage T4 type II DNA
topoisomerase that alter sensitivity to antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide and
an antibacterial quinolone. Cancer Research 58: 1260-1267.
23. Freudenreich, C. H. and Kreuzer, K. N. (1994) Localization of an aminoacridine antitumor agent in a type II
topoisomerase-DNA complex. Proc. Natl. Acad. Sci. USA, 91: 11007-11011.
24. Freudenreich, C. H. and Kreuzer, K. N. (1994) Differentially labeled mutant oligonucleotides for analysis of
protein-DNA interactions. BioTechniques, 16:104-108.
25. Freudenreich, C. H. and Kreuzer, K. N. (1993) Mutational analysis of a type II topoisomerase cleavage site:
distinct requirements for enzyme and inhibitors. EMBO J., 12: 2085-2097.
26. Longmire, J.L., Gee, G.F., Hardekopf, C.L., and Graham, M.A. (1992) Establishing Paternity in Whooping
Cranes (Grus Americana) by DNA analysis. The Auk 109: 522-529. (Hardekopf = maiden name)
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27. Longmire J.L., Kraemer, P.M., Brown, N.C., Hardekopf, C.L. and Deaven, L.L. (1990) A new multi-locus
DNA fingerprinting probe: pV47-2. Nuc. Acids. Res. 18:1658. (Hardekopf = maiden name)
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