Relative Quantification run files need to be imported into a Relative ...

Analysis with SDS2.21. AnalysisAnalysis Settings2. In the Ct Analysis Section of the Analysis settings Window:- from the "Detector:" drop down menu select "All Detectors"- Choose "Automatic Ct" (this will also default you to"Automatic Baseline"). We recommend that you use theautomated Ct and baseline features for optimum data quality,but you can also choose to set each of these manually.3. In the Gene Expression Section:- choose the proper sample1 from the "Calibrator Sample:" dropdown menu.- Choose the proper detector2 from the "Endogenous ControlDetector:" drop down menu (Only detectors defined asEndogenous controls in the task section of the original runfile will be available).- If you wish your exported data to contain the Ct values aswell as the analysis, check the "Export Individual ΔCts" box.- Left click Apply then left click OK.4. AnalysisAnalyze5. FileExport:- From the "Export:" drop down menu, select "Results Table".- In the "From:" section check "All wells" to export data fromall the wells containing data of "Selected Wells" to exportdata from highlighted wells only.- In the "Format:" section select SDS2.2.- Give the file a proper name and make sure the "Files of type:"drop down menu is set to "Tab-delimited Text(*.txt)".- Left click the Export button.This text file should be imported into MS Excel before further use.The data can be copied and pasted or the text file itself can be openedwith (and saved as) an Excel file. From this exported file you shouldhave the individual Ct values (if you choose to export them, see step 3above) as well as the analysis. The RQ column displays the calculatedlevel of gene expression for the replicate group associated with thetest sample. If it is greater than 1, this is a positive fold changeof that value. If the RQ is less than 1, you must divide negative 1 bythat value to obtain the true negative fold change value. This can bedone by entering the following formula into a cell in the Excel file(replace A1 with whichever cell contains the RQ value.=IF(A1