New Feed Enzyme Development - The Poultry Federation

September 2009, ChemGen Corp.In a 42 day broiler trialbirds growthwas significantly improved for both Hemicell® andhomogeneousmannanase treatment over no enzyme control, and there was no significant differencee between thetwoenzyme treatments.Figure 3 – Purified Mannanase used in broiler growth studyBroiler Trial -42 DaysA1.91.87aBWAFC1.841.81bb1.781.75Mannanase from Hemicell® was purified by chromatograpy on DEAE‐Sepharose and Phenyl‐Sepharose usingtraditional methods. Panel A: SDS PAGE gel comparing crude Hemicell® and purified mannanase. Panel B: Weightadjusted feed conversionn vs. treatment. Different lower case letters on bars signify statisticallysignificantdifferences(P < 0.05). Conducted at SPR.These results indicate that mannanase is the component important for improved growth in normalhealthy birds, but the pure enzyme was also tested in the necrotic enteritis disease model at SouthernPoultry Research to besure that mannanase is the component necessary for the even larger percentageimprovements observed vs. infected control . The same lot ofpurified mannanase wasused. The resultsin Figure 4 demonstrate virtually identical performance for purified mannanase and Hemicell®. Thesetwo feeding trials indicate that mannanase is theactive ingredient. More recent evidence comes fromwork witha heat resistant β‐mannanase. Fermentation broth with heat resistant mannanase washeated at83 °C to inactivate all enzymes except mannanase, and then added in pen trials at 100 MU/tonmannanase and bird performance was compared to a control without mannanase and Hemicell®.Performance between normal and heated heat‐resistant mannanaseis the activeingredient incorn‐ soybean Hemicell® was again equivalent in several tests(data not shown). It is clear that the diets.D.M. Anderson and H.Hsiao, New Feed EnzymesPage 9